Bark beetle-associated genomovirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus denbre3

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344A3Q1|A0A344A3Q1_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 3 OX=2230898 PE=3 SV=1
MM1 pKa = 7.82PFACNGGYY9 pKa = 10.45FFVTYY14 pKa = 10.19SQVGSLDD21 pKa = 3.25PFALVDD27 pKa = 3.58HH28 pKa = 6.89FGKK31 pKa = 10.67LGAEE35 pKa = 4.21IIVSLEE41 pKa = 3.83QYY43 pKa = 9.55HH44 pKa = 6.84ASLGVHH50 pKa = 5.28FHH52 pKa = 5.83VFADD56 pKa = 4.64FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84TDD67 pKa = 2.68IFDD70 pKa = 3.27VDD72 pKa = 3.91GFHH75 pKa = 7.48PNISPSRR82 pKa = 11.84GTPEE86 pKa = 3.98AGYY89 pKa = 10.62DD90 pKa = 3.63YY91 pKa = 10.68AIKK94 pKa = 10.92DD95 pKa = 3.59GDD97 pKa = 4.02VVAGGLARR105 pKa = 11.84PSGMVASNRR114 pKa = 11.84AAKK117 pKa = 8.46WHH119 pKa = 6.77SILDD123 pKa = 3.54AEE125 pKa = 4.54TRR127 pKa = 11.84DD128 pKa = 3.66EE129 pKa = 5.25FFALCEE135 pKa = 3.97EE136 pKa = 5.06LDD138 pKa = 3.88PEE140 pKa = 4.71RR141 pKa = 11.84LVCSFGQIQKK151 pKa = 9.89FADD154 pKa = 2.77WRR156 pKa = 11.84FARR159 pKa = 11.84KK160 pKa = 9.04PEE162 pKa = 4.62PYY164 pKa = 10.12ASPNGVFDD172 pKa = 4.3LANYY176 pKa = 9.8GDD178 pKa = 4.07LGDD181 pKa = 3.72WRR183 pKa = 11.84DD184 pKa = 3.42NFLFTDD190 pKa = 3.74TPGRR194 pKa = 11.84SKK196 pKa = 11.09SLILWGPSRR205 pKa = 11.84MGKK208 pKa = 6.86TVWARR213 pKa = 11.84SLGSHH218 pKa = 7.15LYY220 pKa = 10.35FGGIFSARR228 pKa = 11.84NLGDD232 pKa = 4.4DD233 pKa = 4.32GIEE236 pKa = 3.88YY237 pKa = 10.77AVFDD241 pKa = 5.51DD242 pKa = 3.48IAGGIKK248 pKa = 10.03FFPRR252 pKa = 11.84FKK254 pKa = 10.79DD255 pKa = 3.12WLGCQMEE262 pKa = 4.36FMVKK266 pKa = 9.32EE267 pKa = 4.5MYY269 pKa = 10.15RR270 pKa = 11.84DD271 pKa = 3.31PHH273 pKa = 6.37LFRR276 pKa = 11.84WGRR279 pKa = 11.84PAIWIANSDD288 pKa = 3.56PRR290 pKa = 11.84HH291 pKa = 6.58DD292 pKa = 3.89MTHH295 pKa = 7.25DD296 pKa = 4.74DD297 pKa = 4.35IVWLEE302 pKa = 3.92ANCIFVEE309 pKa = 4.12ISSAIFHH316 pKa = 7.19ANTEE320 pKa = 4.19WTGGGSSVDD329 pKa = 3.38QLSQNPAA336 pKa = 3.02

Molecular weight: 37.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344A3Q1|A0A344A3Q1_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 3 OX=2230898 PE=3 SV=1
MM1 pKa = 7.66VYY3 pKa = 8.89RR4 pKa = 11.84TRR6 pKa = 11.84YY7 pKa = 8.73KK8 pKa = 9.94RR9 pKa = 11.84RR10 pKa = 11.84STRR13 pKa = 11.84RR14 pKa = 11.84YY15 pKa = 4.84PTKK18 pKa = 9.93RR19 pKa = 11.84KK20 pKa = 9.26SPARR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84SFVKK30 pKa = 9.9RR31 pKa = 11.84RR32 pKa = 11.84SITRR36 pKa = 11.84TKK38 pKa = 10.59RR39 pKa = 11.84MSARR43 pKa = 11.84KK44 pKa = 8.67ILNVSSVKK52 pKa = 10.37KK53 pKa = 10.12RR54 pKa = 11.84DD55 pKa = 3.72TMLCWTNSTAAAQQGGSTYY74 pKa = 10.84SNAPAVVTGGLADD87 pKa = 3.82TNAQCFLWNATARR100 pKa = 11.84DD101 pKa = 3.77NTSSYY106 pKa = 11.12NGPAGNKK113 pKa = 9.46FNTATRR119 pKa = 11.84TSTNCYY125 pKa = 8.78MVGLSEE131 pKa = 4.89SVEE134 pKa = 4.22IQCNTGMPWQWRR146 pKa = 11.84RR147 pKa = 11.84ICFTMKK153 pKa = 10.4GPSLVPTNAASGVVFTAAAEE173 pKa = 4.28TSNGWQRR180 pKa = 11.84IMNQVVGNPGTNPQYY195 pKa = 11.15TFMQTLFKK203 pKa = 10.71GQCQGDD209 pKa = 4.12WIDD212 pKa = 5.26PITAPVDD219 pKa = 3.26NSRR222 pKa = 11.84ITIKK226 pKa = 10.32YY227 pKa = 9.93DD228 pKa = 3.15KK229 pKa = 10.64VRR231 pKa = 11.84SLASGNEE238 pKa = 3.87DD239 pKa = 3.51GFIRR243 pKa = 11.84TYY245 pKa = 11.05KK246 pKa = 9.83LWHH249 pKa = 6.77PMRR252 pKa = 11.84AMLYY256 pKa = 10.46YY257 pKa = 10.69DD258 pKa = 4.64DD259 pKa = 5.86DD260 pKa = 4.84EE261 pKa = 6.3NGGTEE266 pKa = 4.0VASIQSSTGKK276 pKa = 10.52AGMGDD281 pKa = 4.16YY282 pKa = 10.98YY283 pKa = 10.47IVDD286 pKa = 4.37LFRR289 pKa = 11.84ARR291 pKa = 11.84QGSATADD298 pKa = 3.3QLTIRR303 pKa = 11.84PQSTLYY309 pKa = 8.45WHH311 pKa = 6.63EE312 pKa = 3.99RR313 pKa = 3.41

Molecular weight: 35.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

649

313

336

324.5

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.32 ± 0.009

1.695 ± 0.139

6.626 ± 1.35

3.39 ± 0.723

5.855 ± 1.869

8.629 ± 0.803

2.003 ± 0.855

4.777 ± 0.391

4.006 ± 0.493

5.393 ± 0.777

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.465

4.622 ± 0.908

4.622 ± 0.294

3.39 ± 0.88

7.704 ± 0.979

7.704 ± 0.378

6.626 ± 2.457

5.393 ± 0.024

2.773 ± 0.136

3.698 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski