Mycoplasma equirhinis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A501SBM4|A0A501SBM4_9MOLU Site-specific integrase (Fragment) OS=Mycoplasma equirhinis OX=92402 GN=FJM08_03400 PE=4 SV=1
VV1 pKa = 6.91NNPEE5 pKa = 4.13QFWDD9 pKa = 3.89LLCAGRR15 pKa = 11.84SGIVRR20 pKa = 11.84VPAQRR25 pKa = 11.84WDD27 pKa = 3.14ADD29 pKa = 3.21AYY31 pKa = 10.83YY32 pKa = 10.47CDD34 pKa = 4.12DD35 pKa = 3.59HH36 pKa = 6.69TVPGTICSTEE46 pKa = 3.92GGFLTSWQPDD56 pKa = 3.21EE57 pKa = 4.8FDD59 pKa = 4.73AEE61 pKa = 4.45FFSIPPRR68 pKa = 11.84EE69 pKa = 4.16AAAMDD74 pKa = 3.63PQQRR78 pKa = 11.84LFIEE82 pKa = 4.66VAWDD86 pKa = 3.33ALDD89 pKa = 3.63YY90 pKa = 11.49AGVPQHH96 pKa = 6.54TIRR99 pKa = 11.84GTQTSVFVGVTAYY112 pKa = 10.77DD113 pKa = 3.52YY114 pKa = 10.72MLTLAGRR121 pKa = 11.84LRR123 pKa = 11.84PVDD126 pKa = 4.09LDD128 pKa = 3.86AYY130 pKa = 9.93IPTGNSANFAAGRR143 pKa = 11.84LAYY146 pKa = 9.58ILGARR151 pKa = 11.84GPAVVIDD158 pKa = 4.19TACSSSLVAVHH169 pKa = 6.79LL170 pKa = 4.33

Molecular weight:
18.5 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A501SP70|A0A501SP70_9MOLU Leucyl aminopeptidase family protein OS=Mycoplasma equirhinis OX=92402 GN=FJM08_01160 PE=3 SV=1
MM1 pKa = 7.54AKK3 pKa = 9.93SSKK6 pKa = 9.85RR7 pKa = 11.84RR8 pKa = 11.84PAPEE12 pKa = 4.13KK13 pKa = 9.63PVKK16 pKa = 9.16TRR18 pKa = 11.84KK19 pKa = 9.67CVFCAKK25 pKa = 9.82KK26 pKa = 10.56DD27 pKa = 3.53QAIDD31 pKa = 3.74YY32 pKa = 10.18KK33 pKa = 10.75DD34 pKa = 3.25TALLRR39 pKa = 11.84TYY41 pKa = 10.13ISEE44 pKa = 4.05RR45 pKa = 11.84GKK47 pKa = 9.89IRR49 pKa = 11.84ARR51 pKa = 11.84RR52 pKa = 11.84VTGNCVQHH60 pKa = 6.07QRR62 pKa = 11.84DD63 pKa = 3.18IALAVTNARR72 pKa = 3.98

Molecular weight:
8.22 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

613

0

613

212910

24

3395

347.3

39.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.909 ± 0.12

0.645 ± 0.032

5.339 ± 0.087

7.113 ± 0.129

5.293 ± 0.111

4.678 ± 0.097

1.448 ± 0.042

10.044 ± 0.123

10.529 ± 0.162

9.468 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.75 ± 0.049

7.785 ± 0.113

2.668 ± 0.074

3.059 ± 0.049

3.042 ± 0.079

6.449 ± 0.063

4.938 ± 0.064

4.92 ± 0.09

0.903 ± 0.033

4.019 ± 0.067

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski