Mycoplasma equirhinis
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 613 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A501SBM4|A0A501SBM4_9MOLU Site-specific integrase (Fragment) OS=Mycoplasma equirhinis OX=92402 GN=FJM08_03400 PE=4 SV=1
VV1 pKa = 6.91 NNPEE5 pKa = 4.13 QFWDD9 pKa = 3.89 LLCAGRR15 pKa = 11.84 SGIVRR20 pKa = 11.84 VPAQRR25 pKa = 11.84 WDD27 pKa = 3.14 ADD29 pKa = 3.21 AYY31 pKa = 10.83 YY32 pKa = 10.47 CDD34 pKa = 4.12 DD35 pKa = 3.59 HH36 pKa = 6.69 TVPGTICSTEE46 pKa = 3.92 GGFLTSWQPDD56 pKa = 3.21 EE57 pKa = 4.8 FDD59 pKa = 4.73 AEE61 pKa = 4.45 FFSIPPRR68 pKa = 11.84 EE69 pKa = 4.16 AAAMDD74 pKa = 3.63 PQQRR78 pKa = 11.84 LFIEE82 pKa = 4.66 VAWDD86 pKa = 3.33 ALDD89 pKa = 3.63 YY90 pKa = 11.49 AGVPQHH96 pKa = 6.54 TIRR99 pKa = 11.84 GTQTSVFVGVTAYY112 pKa = 10.77 DD113 pKa = 3.52 YY114 pKa = 10.72 MLTLAGRR121 pKa = 11.84 LRR123 pKa = 11.84 PVDD126 pKa = 4.09 LDD128 pKa = 3.86 AYY130 pKa = 9.93 IPTGNSANFAAGRR143 pKa = 11.84 LAYY146 pKa = 9.58 ILGARR151 pKa = 11.84 GPAVVIDD158 pKa = 4.19 TACSSSLVAVHH169 pKa = 6.79 LL170 pKa = 4.33
Molecular weight: 18.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.062
IPC2_protein 4.368
IPC_protein 4.304
Toseland 4.113
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.24
Rodwell 4.139
Grimsley 4.024
Solomon 4.291
Lehninger 4.253
Nozaki 4.418
DTASelect 4.673
Thurlkill 4.151
EMBOSS 4.253
Sillero 4.431
Patrickios 2.015
IPC_peptide 4.291
IPC2_peptide 4.418
IPC2.peptide.svr19 4.326
Protein with the highest isoelectric point:
>tr|A0A501SP70|A0A501SP70_9MOLU Leucyl aminopeptidase family protein OS=Mycoplasma equirhinis OX=92402 GN=FJM08_01160 PE=3 SV=1
MM1 pKa = 7.54 AKK3 pKa = 9.93 SSKK6 pKa = 9.85 RR7 pKa = 11.84 RR8 pKa = 11.84 PAPEE12 pKa = 4.13 KK13 pKa = 9.63 PVKK16 pKa = 9.16 TRR18 pKa = 11.84 KK19 pKa = 9.67 CVFCAKK25 pKa = 9.82 KK26 pKa = 10.56 DD27 pKa = 3.53 QAIDD31 pKa = 3.74 YY32 pKa = 10.18 KK33 pKa = 10.75 DD34 pKa = 3.25 TALLRR39 pKa = 11.84 TYY41 pKa = 10.13 ISEE44 pKa = 4.05 RR45 pKa = 11.84 GKK47 pKa = 9.89 IRR49 pKa = 11.84 ARR51 pKa = 11.84 RR52 pKa = 11.84 VTGNCVQHH60 pKa = 6.07 QRR62 pKa = 11.84 DD63 pKa = 3.18 IALAVTNARR72 pKa = 3.98
Molecular weight: 8.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.706
IPC_protein 10.233
Toseland 10.847
ProMoST 10.687
Dawson 10.921
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.272
Grimsley 10.95
Solomon 11.023
Lehninger 11.008
Nozaki 10.833
DTASelect 10.57
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.847
Patrickios 11.023
IPC_peptide 11.038
IPC2_peptide 9.604
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
613
0
613
212910
24
3395
347.3
39.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.909 ± 0.12
0.645 ± 0.032
5.339 ± 0.087
7.113 ± 0.129
5.293 ± 0.111
4.678 ± 0.097
1.448 ± 0.042
10.044 ± 0.123
10.529 ± 0.162
9.468 ± 0.086
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.75 ± 0.049
7.785 ± 0.113
2.668 ± 0.074
3.059 ± 0.049
3.042 ± 0.079
6.449 ± 0.063
4.938 ± 0.064
4.92 ± 0.09
0.903 ± 0.033
4.019 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here