Amino acid dipepetide frequency for Ruminococcus sp. CAG:382

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.223AlaAla: 9.223 ± 0.166	1.523AlaCys: 1.523 ± 0.05	5.901AlaAsp: 5.901 ± 0.103	6.425AlaGlu: 6.425 ± 0.14	4.204AlaPhe: 4.204 ± 0.095	5.969AlaGly: 5.969 ± 0.116	1.407AlaHis: 1.407 ± 0.056	5.055AlaIle: 5.055 ± 0.099	5.153AlaLys: 5.153 ± 0.113	8.47AlaLeu: 8.47 ± 0.136	2.201AlaMet: 2.201 ± 0.075	2.704AlaAsn: 2.704 ± 0.077	2.438AlaPro: 2.438 ± 0.075	1.794AlaGln: 1.794 ± 0.057	3.404AlaArg: 3.404 ± 0.09	5.057AlaSer: 5.057 ± 0.111	3.693AlaThr: 3.693 ± 0.085	7.941AlaVal: 7.941 ± 0.153	0.489AlaTrp: 0.489 ± 0.032	3.097AlaTyr: 3.097 ± 0.082	0.0AlaXaa: 0.0 ± 0.0
Cys	1.658CysAla: 1.658 ± 0.061	0.379CysCys: 0.379 ± 0.03	1.385CysAsp: 1.385 ± 0.052	1.146CysGlu: 1.146 ± 0.049	0.814CysPhe: 0.814 ± 0.041	2.162CysGly: 2.162 ± 0.077	0.365CysHis: 0.365 ± 0.028	1.153CysIle: 1.153 ± 0.048	0.84CysLys: 0.84 ± 0.046	1.388CysLeu: 1.388 ± 0.052	0.407CysMet: 0.407 ± 0.027	0.696CysAsn: 0.696 ± 0.035	0.724CysPro: 0.724 ± 0.036	0.233CysGln: 0.233 ± 0.019	1.137CysArg: 1.137 ± 0.053	1.077CysSer: 1.077 ± 0.042	1.027CysThr: 1.027 ± 0.046	1.333CysVal: 1.333 ± 0.057	0.1CysTrp: 0.1 ± 0.013	0.585CysTyr: 0.585 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.162AspAla: 5.162 ± 0.101	1.12AspCys: 1.12 ± 0.05	3.926AspAsp: 3.926 ± 0.107	4.916AspGlu: 4.916 ± 0.119	3.004AspPhe: 3.004 ± 0.075	6.188AspGly: 6.188 ± 0.138	0.694AspHis: 0.694 ± 0.04	5.075AspIle: 5.075 ± 0.093	3.598AspLys: 3.598 ± 0.092	3.928AspLeu: 3.928 ± 0.089	1.766AspMet: 1.766 ± 0.06	2.553AspAsn: 2.553 ± 0.072	2.019AspPro: 2.019 ± 0.061	0.648AspGln: 0.648 ± 0.032	2.889AspArg: 2.889 ± 0.074	4.004AspSer: 4.004 ± 0.094	3.313AspThr: 3.313 ± 0.071	4.355AspVal: 4.355 ± 0.095	0.494AspTrp: 0.494 ± 0.035	2.66AspTyr: 2.66 ± 0.083	0.002AspXaa: 0.002 ± 0.002
Glu	5.244GluAla: 5.244 ± 0.107	0.937GluCys: 0.937 ± 0.042	2.925GluAsp: 2.925 ± 0.086	4.354GluGlu: 4.354 ± 0.102	2.379GluPhe: 2.379 ± 0.064	3.663GluGly: 3.663 ± 0.097	1.146GluHis: 1.146 ± 0.054	4.907GluIle: 4.907 ± 0.103	5.897GluLys: 5.897 ± 0.124	6.073GluLeu: 6.073 ± 0.116	1.862GluMet: 1.862 ± 0.069	4.076GluAsn: 4.076 ± 0.084	1.932GluPro: 1.932 ± 0.08	1.962GluGln: 1.962 ± 0.068	3.526GluArg: 3.526 ± 0.11	3.837GluSer: 3.837 ± 0.097	3.621GluThr: 3.621 ± 0.091	3.504GluVal: 3.504 ± 0.087	0.574GluTrp: 0.574 ± 0.035	3.226GluTyr: 3.226 ± 0.089	0.0GluXaa: 0.0 ± 0.0
Phe	4.152PheAla: 4.152 ± 0.095	1.031PheCys: 1.031 ± 0.049	3.35PheAsp: 3.35 ± 0.082	2.973PheGlu: 2.973 ± 0.077	2.203PhePhe: 2.203 ± 0.087	3.752PheGly: 3.752 ± 0.076	0.696PheHis: 0.696 ± 0.042	2.91PheIle: 2.91 ± 0.077	1.658PheLys: 1.658 ± 0.062	3.284PheLeu: 3.284 ± 0.101	1.137PheMet: 1.137 ± 0.047	1.571PheAsn: 1.571 ± 0.062	1.51PhePro: 1.51 ± 0.054	0.668PheGln: 0.668 ± 0.034	2.253PheArg: 2.253 ± 0.064	3.495PheSer: 3.495 ± 0.095	2.673PheThr: 2.673 ± 0.076	3.289PheVal: 3.289 ± 0.101	0.313PheTrp: 0.313 ± 0.023	1.664PheTyr: 1.664 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	5.349GlyAla: 5.349 ± 0.122	1.555GlyCys: 1.555 ± 0.064	4.657GlyAsp: 4.657 ± 0.096	4.916GlyGlu: 4.916 ± 0.102	3.456GlyPhe: 3.456 ± 0.087	5.577GlyGly: 5.577 ± 0.133	1.209GlyHis: 1.209 ± 0.051	5.857GlyIle: 5.857 ± 0.111	5.475GlyLys: 5.475 ± 0.112	5.333GlyLeu: 5.333 ± 0.119	2.23GlyMet: 2.23 ± 0.07	3.335GlyAsn: 3.335 ± 0.081	0.95GlyPro: 0.95 ± 0.046	1.377GlyGln: 1.377 ± 0.047	3.426GlyArg: 3.426 ± 0.086	4.572GlySer: 4.572 ± 0.1	4.365GlyThr: 4.365 ± 0.09	5.59GlyVal: 5.59 ± 0.116	0.676GlyTrp: 0.676 ± 0.039	3.319GlyTyr: 3.319 ± 0.077	0.006GlyXaa: 0.006 ± 0.003
His	1.122HisAla: 1.122 ± 0.048	0.359HisCys: 0.359 ± 0.028	1.061HisAsp: 1.061 ± 0.045	0.994HisGlu: 0.994 ± 0.044	0.838HisPhe: 0.838 ± 0.039	1.385HisGly: 1.385 ± 0.06	0.328HisHis: 0.328 ± 0.028	1.385HisIle: 1.385 ± 0.048	0.77HisLys: 0.77 ± 0.039	1.199HisLeu: 1.199 ± 0.047	0.42HisMet: 0.42 ± 0.029	0.711HisAsn: 0.711 ± 0.041	0.833HisPro: 0.833 ± 0.042	0.27HisGln: 0.27 ± 0.02	0.911HisArg: 0.911 ± 0.043	1.005HisSer: 1.005 ± 0.048	1.085HisThr: 1.085 ± 0.04	0.929HisVal: 0.929 ± 0.041	0.131HisTrp: 0.131 ± 0.017	0.646HisTyr: 0.646 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.436IleAla: 6.436 ± 0.12	1.54IleCys: 1.54 ± 0.063	4.341IleAsp: 4.341 ± 0.096	4.078IleGlu: 4.078 ± 0.092	2.764IlePhe: 2.764 ± 0.071	4.616IleGly: 4.616 ± 0.087	1.038IleHis: 1.038 ± 0.051	4.981IleIle: 4.981 ± 0.098	3.882IleLys: 3.882 ± 0.097	5.346IleLeu: 5.346 ± 0.11	1.62IleMet: 1.62 ± 0.059	2.978IleAsn: 2.978 ± 0.084	2.965IlePro: 2.965 ± 0.073	1.198IleGln: 1.198 ± 0.052	3.558IleArg: 3.558 ± 0.092	5.192IleSer: 5.192 ± 0.103	4.359IleThr: 4.359 ± 0.095	4.913IleVal: 4.913 ± 0.101	0.411IleTrp: 0.411 ± 0.028	2.704IleTyr: 2.704 ± 0.069	0.0IleXaa: 0.0 ± 0.0
Lys	5.244LysAla: 5.244 ± 0.102	0.922LysCys: 0.922 ± 0.048	3.139LysAsp: 3.139 ± 0.084	3.88LysGlu: 3.88 ± 0.105	1.786LysPhe: 1.786 ± 0.063	3.728LysGly: 3.728 ± 0.094	0.972LysHis: 0.972 ± 0.039	3.996LysIle: 3.996 ± 0.088	4.99LysLys: 4.99 ± 0.103	5.47LysLeu: 5.47 ± 0.112	1.777LysMet: 1.777 ± 0.059	3.628LysAsn: 3.628 ± 0.084	2.249LysPro: 2.249 ± 0.065	1.786LysGln: 1.786 ± 0.061	3.572LysArg: 3.572 ± 0.089	3.559LysSer: 3.559 ± 0.089	4.122LysThr: 4.122 ± 0.107	3.243LysVal: 3.243 ± 0.08	0.461LysTrp: 0.461 ± 0.031	2.654LysTyr: 2.654 ± 0.08	0.002LysXaa: 0.002 ± 0.002
Leu	7.509LeuAla: 7.509 ± 0.136	2.075LeuCys: 2.075 ± 0.069	5.011LeuAsp: 5.011 ± 0.11	5.061LeuGlu: 5.061 ± 0.104	4.25LeuPhe: 4.25 ± 0.11	5.668LeuGly: 5.668 ± 0.115	1.386LeuHis: 1.386 ± 0.054	5.608LeuIle: 5.608 ± 0.132	5.085LeuLys: 5.085 ± 0.115	7.972LeuLeu: 7.972 ± 0.161	2.273LeuMet: 2.273 ± 0.069	3.058LeuAsn: 3.058 ± 0.07	3.806LeuPro: 3.806 ± 0.099	1.683LeuGln: 1.683 ± 0.058	4.487LeuArg: 4.487 ± 0.089	6.621LeuSer: 6.621 ± 0.129	5.325LeuThr: 5.325 ± 0.115	5.125LeuVal: 5.125 ± 0.116	0.711LeuTrp: 0.711 ± 0.041	3.271LeuTyr: 3.271 ± 0.083	0.0LeuXaa: 0.0 ± 0.0
Met	1.853MetAla: 1.853 ± 0.052	0.413MetCys: 0.413 ± 0.028	1.099MetAsp: 1.099 ± 0.045	1.405MetGlu: 1.405 ± 0.044	1.057MetPhe: 1.057 ± 0.046	1.473MetGly: 1.473 ± 0.059	0.442MetHis: 0.442 ± 0.031	1.777MetIle: 1.777 ± 0.056	2.354MetLys: 2.354 ± 0.067	2.915MetLeu: 2.915 ± 0.078	0.665MetMet: 0.665 ± 0.037	1.427MetAsn: 1.427 ± 0.049	1.231MetPro: 1.231 ± 0.052	0.777MetGln: 0.777 ± 0.036	1.372MetArg: 1.372 ± 0.055	1.825MetSer: 1.825 ± 0.061	1.875MetThr: 1.875 ± 0.057	1.244MetVal: 1.244 ± 0.046	0.189MetTrp: 0.189 ± 0.018	0.888MetTyr: 0.888 ± 0.045	0.0MetXaa: 0.0 ± 0.0
Asn	3.989AsnAla: 3.989 ± 0.094	0.629AsnCys: 0.629 ± 0.037	2.652AsnAsp: 2.652 ± 0.076	2.751AsnGlu: 2.751 ± 0.064	1.42AsnPhe: 1.42 ± 0.051	4.365AsnGly: 4.365 ± 0.098	0.644AsnHis: 0.644 ± 0.041	3.284AsnIle: 3.284 ± 0.075	2.073AsnLys: 2.073 ± 0.061	2.956AsnLeu: 2.956 ± 0.083	1.09AsnMet: 1.09 ± 0.037	1.788AsnAsn: 1.788 ± 0.07	1.775AsnPro: 1.775 ± 0.058	0.74AsnGln: 0.74 ± 0.037	2.064AsnArg: 2.064 ± 0.061	2.471AsnSer: 2.471 ± 0.079	2.573AsnThr: 2.573 ± 0.074	2.888AsnVal: 2.888 ± 0.068	0.344AsnTrp: 0.344 ± 0.026	1.62AsnTyr: 1.62 ± 0.067	0.0AsnXaa: 0.0 ± 0.0
Pro	2.928ProAla: 2.928 ± 0.083	0.592ProCys: 0.592 ± 0.031	3.036ProAsp: 3.036 ± 0.078	3.391ProGlu: 3.391 ± 0.089	1.729ProPhe: 1.729 ± 0.064	1.899ProGly: 1.899 ± 0.057	0.637ProHis: 0.637 ± 0.035	1.81ProIle: 1.81 ± 0.056	1.77ProLys: 1.77 ± 0.057	2.897ProLeu: 2.897 ± 0.087	0.801ProMet: 0.801 ± 0.037	1.107ProAsn: 1.107 ± 0.046	0.981ProPro: 0.981 ± 0.044	0.805ProGln: 0.805 ± 0.037	1.198ProArg: 1.198 ± 0.047	2.371ProSer: 2.371 ± 0.082	1.932ProThr: 1.932 ± 0.056	2.984ProVal: 2.984 ± 0.067	0.259ProTrp: 0.259 ± 0.023	1.472ProTyr: 1.472 ± 0.054	0.0ProXaa: 0.0 ± 0.0
Gln	1.559GlnAla: 1.559 ± 0.06	0.313GlnCys: 0.313 ± 0.024	0.792GlnAsp: 0.792 ± 0.036	0.938GlnGlu: 0.938 ± 0.048	0.74GlnPhe: 0.74 ± 0.039	1.153GlnGly: 1.153 ± 0.048	0.405GlnHis: 0.405 ± 0.031	1.483GlnIle: 1.483 ± 0.05	1.592GlnLys: 1.592 ± 0.058	2.145GlnLeu: 2.145 ± 0.07	0.642GlnMet: 0.642 ± 0.036	1.212GlnAsn: 1.212 ± 0.051	0.748GlnPro: 0.748 ± 0.038	0.613GlnGln: 0.613 ± 0.04	1.146GlnArg: 1.146 ± 0.051	1.551GlnSer: 1.551 ± 0.048	1.323GlnThr: 1.323 ± 0.05	1.098GlnVal: 1.098 ± 0.045	0.185GlnTrp: 0.185 ± 0.018	0.955GlnTyr: 0.955 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	3.421ArgAla: 3.421 ± 0.092	0.792ArgCys: 0.792 ± 0.04	2.834ArgAsp: 2.834 ± 0.071	3.498ArgGlu: 3.498 ± 0.089	2.628ArgPhe: 2.628 ± 0.068	2.719ArgGly: 2.719 ± 0.071	1.029ArgHis: 1.029 ± 0.041	3.817ArgIle: 3.817 ± 0.094	3.228ArgLys: 3.228 ± 0.086	4.827ArgLeu: 4.827 ± 0.102	1.47ArgMet: 1.47 ± 0.053	2.212ArgAsn: 2.212 ± 0.069	1.357ArgPro: 1.357 ± 0.052	1.314ArgGln: 1.314 ± 0.05	3.265ArgArg: 3.265 ± 0.088	2.876ArgSer: 2.876 ± 0.069	2.756ArgThr: 2.756 ± 0.068	3.193ArgVal: 3.193 ± 0.079	0.337ArgTrp: 0.337 ± 0.025	2.167ArgTyr: 2.167 ± 0.063	0.004ArgXaa: 0.004 ± 0.002
Ser	6.082SerAla: 6.082 ± 0.125	1.166SerCys: 1.166 ± 0.047	4.652SerAsp: 4.652 ± 0.114	4.629SerGlu: 4.629 ± 0.111	3.099SerPhe: 3.099 ± 0.078	5.964SerGly: 5.964 ± 0.131	1.057SerHis: 1.057 ± 0.046	3.676SerIle: 3.676 ± 0.088	3.441SerLys: 3.441 ± 0.092	5.807SerLeu: 5.807 ± 0.101	1.592SerMet: 1.592 ± 0.056	2.075SerAsn: 2.075 ± 0.066	2.201SerPro: 2.201 ± 0.068	1.425SerGln: 1.425 ± 0.056	3.093SerArg: 3.093 ± 0.075	4.354SerSer: 4.354 ± 0.11	3.147SerThr: 3.147 ± 0.081	5.536SerVal: 5.536 ± 0.104	0.435SerTrp: 0.435 ± 0.031	2.63SerTyr: 2.63 ± 0.071	0.002SerXaa: 0.002 ± 0.002
Thr	5.734ThrAla: 5.734 ± 0.091	0.742ThrCys: 0.742 ± 0.038	4.17ThrAsp: 4.17 ± 0.086	3.77ThrGlu: 3.77 ± 0.084	2.384ThrPhe: 2.384 ± 0.068	4.585ThrGly: 4.585 ± 0.092	1.025ThrHis: 1.025 ± 0.043	3.219ThrIle: 3.219 ± 0.091	2.671ThrLys: 2.671 ± 0.07	5.47ThrLeu: 5.47 ± 0.099	1.199ThrMet: 1.199 ± 0.047	1.938ThrAsn: 1.938 ± 0.07	2.565ThrPro: 2.565 ± 0.072	1.075ThrGln: 1.075 ± 0.049	2.195ThrArg: 2.195 ± 0.062	3.261ThrSer: 3.261 ± 0.084	2.882ThrThr: 2.882 ± 0.079	6.271ThrVal: 6.271 ± 0.117	0.396ThrTrp: 0.396 ± 0.031	2.238ThrTyr: 2.238 ± 0.072	0.004ThrXaa: 0.004 ± 0.003
Val	5.718ValAla: 5.718 ± 0.111	1.623ValCys: 1.623 ± 0.056	3.919ValAsp: 3.919 ± 0.093	3.619ValGlu: 3.619 ± 0.077	3.556ValPhe: 3.556 ± 0.094	4.657ValGly: 4.657 ± 0.1	1.003ValHis: 1.003 ± 0.044	5.36ValIle: 5.36 ± 0.12	4.159ValLys: 4.159 ± 0.094	6.38ValLeu: 6.38 ± 0.12	2.014ValMet: 2.014 ± 0.061	2.823ValAsn: 2.823 ± 0.078	2.73ValPro: 2.73 ± 0.069	1.192ValGln: 1.192 ± 0.046	3.739ValArg: 3.739 ± 0.088	5.734ValSer: 5.734 ± 0.114	4.824ValThr: 4.824 ± 0.096	4.892ValVal: 4.892 ± 0.108	0.578ValTrp: 0.578 ± 0.036	2.854ValTyr: 2.854 ± 0.069	0.002ValXaa: 0.002 ± 0.002
Trp	0.452TrpAla: 0.452 ± 0.033	0.181TrpCys: 0.181 ± 0.022	0.452TrpAsp: 0.452 ± 0.026	0.492TrpGlu: 0.492 ± 0.027	0.385TrpPhe: 0.385 ± 0.029	0.546TrpGly: 0.546 ± 0.031	0.178TrpHis: 0.178 ± 0.021	0.465TrpIle: 0.465 ± 0.031	0.468TrpLys: 0.468 ± 0.03	0.79TrpLeu: 0.79 ± 0.043	0.198TrpMet: 0.198 ± 0.02	0.363TrpAsn: 0.363 ± 0.027	0.118TrpPro: 0.118 ± 0.016	0.292TrpGln: 0.292 ± 0.024	0.328TrpArg: 0.328 ± 0.026	0.444TrpSer: 0.444 ± 0.026	0.409TrpThr: 0.409 ± 0.028	0.411TrpVal: 0.411 ± 0.031	0.1TrpTrp: 0.1 ± 0.013	0.392TrpTyr: 0.392 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.304TyrAla: 3.304 ± 0.088	0.714TyrCys: 0.714 ± 0.038	3.015TyrAsp: 3.015 ± 0.073	2.408TyrGlu: 2.408 ± 0.072	1.886TyrPhe: 1.886 ± 0.058	3.16TyrGly: 3.16 ± 0.084	0.718TyrHis: 0.718 ± 0.036	3.1TyrIle: 3.1 ± 0.073	2.073TyrLys: 2.073 ± 0.065	3.332TyrLeu: 3.332 ± 0.087	0.916TyrMet: 0.916 ± 0.039	1.86TyrAsn: 1.86 ± 0.071	1.462TyrPro: 1.462 ± 0.053	0.74TyrGln: 0.74 ± 0.034	2.182TyrArg: 2.182 ± 0.069	2.752TyrSer: 2.752 ± 0.066	2.495TyrThr: 2.495 ± 0.076	2.636TyrVal: 2.636 ± 0.08	0.283TyrTrp: 0.283 ± 0.025	1.888TyrTyr: 1.888 ± 0.066	0.004TyrXaa: 0.004 ± 0.002
Xaa	0.007XaaAla: 0.007 ± 0.004	0.002XaaCys: 0.002 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.002	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.003	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.024XaaXaa: 0.024 ± 0.008

Statistics based on 1853 proteins (540255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---