Mesobacillus campisalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Mesobacillus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4713 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2SW21|A0A0M2SW21_9BACI Uncharacterized protein OS=Mesobacillus campisalis OX=1408103 GN=WQ57_12055 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.12KK3 pKa = 10.46VYY5 pKa = 10.86GLMVQAGDD13 pKa = 4.0ANEE16 pKa = 4.1MLWDD20 pKa = 3.8RR21 pKa = 11.84GVWEE25 pKa = 4.2TEE27 pKa = 3.94EE28 pKa = 3.85AAKK31 pKa = 10.38EE32 pKa = 3.91YY33 pKa = 10.5LQSEE37 pKa = 4.37MRR39 pKa = 11.84NINGIWVTEE48 pKa = 3.99LKK50 pKa = 11.18VNDD53 pKa = 5.1SIPEE57 pKa = 3.88AAEE60 pKa = 3.98PEE62 pKa = 4.2AEE64 pKa = 4.6EE65 pKa = 5.3MILCDD70 pKa = 4.51LCGIKK75 pKa = 10.53YY76 pKa = 10.47NPADD80 pKa = 3.59VNVTDD85 pKa = 6.06FEE87 pKa = 4.71DD88 pKa = 4.22AVCINCEE95 pKa = 4.06PEE97 pKa = 4.48YY98 pKa = 10.11LTQEE102 pKa = 3.95NMLL105 pKa = 4.29

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2SVA8|A0A0M2SVA8_9BACI RNA methyltransferase OS=Mesobacillus campisalis OX=1408103 GN=WQ57_12980 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSSTGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4713

0

4713

1386986

26

1934

294.3

32.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.757 ± 0.039

0.713 ± 0.011

4.897 ± 0.026

7.606 ± 0.047

4.558 ± 0.032

7.52 ± 0.028

2.102 ± 0.017

7.335 ± 0.036

6.518 ± 0.034

9.962 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.837 ± 0.017

4.058 ± 0.027

3.842 ± 0.024

3.582 ± 0.023

4.28 ± 0.024

5.964 ± 0.023

5.034 ± 0.022

7.025 ± 0.028

1.04 ± 0.013

3.369 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski