Piloderma croceum (strain F 1598)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21360 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C3GEX2|A0A0C3GEX2_PILCF Uncharacterized protein OS=Piloderma croceum (strain F 1598) OX=765440 GN=PILCRDRAFT_175946 PE=4 SV=1
MM1 pKa = 8.0 DD2 pKa = 5.61 LNGSLHH8 pKa = 7.36 DD9 pKa = 4.17 FFLDD13 pKa = 3.71 YY14 pKa = 11.16 EE15 pKa = 4.23 IDD17 pKa = 3.24 QWLNTQGQGDD27 pKa = 4.13 GAALEE32 pKa = 4.09 QAMAALSNHH41 pKa = 6.77 LDD43 pKa = 3.38 FADD46 pKa = 3.47 WFDD49 pKa = 4.17 LLGFNVDD56 pKa = 3.44 PNVANRR62 pKa = 11.84 AIPGTSSGNEE72 pKa = 3.81 APTSPSAPGSTSSQPVSGSTSLQPSATFLNSYY104 pKa = 9.17 YY105 pKa = 10.96 GSSTTLNLNISAALSMGIPSTSVTNFPISSLPSTVTDD142 pKa = 3.54 LTFDD146 pKa = 3.47 TVNPVWAHH154 pKa = 6.43 LLHH157 pKa = 6.65 PTFDD161 pKa = 3.54 MAITIALQDD170 pKa = 4.21 HH171 pKa = 6.14 SLGWLPDD178 pKa = 3.27
Molecular weight: 18.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.706
IPC_protein 3.694
Toseland 3.465
ProMoST 3.834
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.139
Thurlkill 3.554
EMBOSS 3.719
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A0C3EF86|A0A0C3EF86_PILCF Uncharacterized protein OS=Piloderma croceum (strain F 1598) OX=765440 GN=PILCRDRAFT_830415 PE=4 SV=1
FF1 pKa = 6.99 TRR3 pKa = 11.84 RR4 pKa = 11.84 APNRR8 pKa = 11.84 ATTARR13 pKa = 11.84 FRR15 pKa = 11.84 PFSPNRR21 pKa = 11.84 LSLASRR27 pKa = 11.84 LRR29 pKa = 11.84 TQRR32 pKa = 11.84 PTAATASSAPPHH44 pKa = 6.38 HH45 pKa = 7.14 PPSPFSIPISNGAPEE60 pKa = 4.84 IKK62 pKa = 9.75 PQRR65 pKa = 11.84 LDD67 pKa = 3.47 FGFLALGPLSGLAFANAMPHH87 pKa = 5.56 HH88 pKa = 6.27 HH89 pKa = 7.26 HH90 pKa = 6.47 NLISTTAPPPFTALHH105 pKa = 5.68 PHH107 pKa = 5.39 FQRR110 pKa = 11.84 RR111 pKa = 11.84 ARR113 pKa = 11.84 TQAPVAQFRR122 pKa = 11.84 VLTLNN127 pKa = 3.52
Molecular weight: 13.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.369
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.071
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.106
Grimsley 12.603
Solomon 13.071
Lehninger 12.969
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.071
Sillero 12.559
Patrickios 11.842
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21351
9
21360
7081032
49
3925
331.5
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.902 ± 0.015
1.482 ± 0.007
5.716 ± 0.014
5.585 ± 0.018
3.818 ± 0.012
6.309 ± 0.016
2.802 ± 0.011
5.303 ± 0.014
4.671 ± 0.016
9.341 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.007
3.721 ± 0.01
6.145 ± 0.021
3.761 ± 0.011
5.999 ± 0.016
8.765 ± 0.024
6.076 ± 0.011
6.228 ± 0.013
1.481 ± 0.007
2.68 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here