Torque teno midi virus 12
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLZ6|A7VLZ6_9VIRU Capsid protein OS=Torque teno midi virus 12 OX=2065053 PE=3 SV=1
MM1 pKa = 7.68 SIFYY5 pKa = 10.48 KK6 pKa = 8.57 PAKK9 pKa = 10.01 YY10 pKa = 9.94 NGHH13 pKa = 6.35 TKK15 pKa = 8.88 QQMWMSVIADD25 pKa = 3.81 FHH27 pKa = 8.93 DD28 pKa = 4.2 PMCNCWHH35 pKa = 7.12 PFGHH39 pKa = 7.09 LLDD42 pKa = 4.93 IIFPEE47 pKa = 4.28 GHH49 pKa = 7.13 KK50 pKa = 11.1 DD51 pKa = 3.3 RR52 pKa = 11.84 DD53 pKa = 3.46 KK54 pKa = 11.21 TIAQIITRR62 pKa = 11.84 DD63 pKa = 4.07 SKK65 pKa = 10.92 CHH67 pKa = 6.45 SGGEE71 pKa = 4.18 DD72 pKa = 3.11 EE73 pKa = 4.96 GASGQTEE80 pKa = 3.97 DD81 pKa = 4.38 LHH83 pKa = 8.65 IKK85 pKa = 8.78 EE86 pKa = 4.85 EE87 pKa = 4.43 RR88 pKa = 11.84 DD89 pKa = 3.55 TPADD93 pKa = 3.6 TTGGMAEE100 pKa = 4.51 EE101 pKa = 4.45 EE102 pKa = 4.36 TTDD105 pKa = 3.58 ALLAAAVEE113 pKa = 4.28 NFEE116 pKa = 4.35 RR117 pKa = 5.57
Molecular weight: 13.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.793
IPC2_protein 4.825
IPC_protein 4.749
Toseland 4.66
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.622
Rodwell 4.635
Grimsley 4.584
Solomon 4.736
Lehninger 4.698
Nozaki 4.863
DTASelect 5.029
Thurlkill 4.673
EMBOSS 4.66
Sillero 4.914
Patrickios 3.846
IPC_peptide 4.736
IPC2_peptide 4.914
IPC2.peptide.svr19 4.821
Protein with the highest isoelectric point:
>tr|A7VLZ6|A7VLZ6_9VIRU Capsid protein OS=Torque teno midi virus 12 OX=2065053 PE=3 SV=1
MM1 pKa = 7.33 PFWWRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 FWTNRR15 pKa = 11.84 RR16 pKa = 11.84 FTYY19 pKa = 9.93 KK20 pKa = 9.74 RR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 9.45 RR24 pKa = 11.84 YY25 pKa = 7.52 PRR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 7.39 NRR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 9.35 GRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 NYY39 pKa = 9.67 RR40 pKa = 11.84 RR41 pKa = 11.84 PARR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 9.07 LRR51 pKa = 11.84 KK52 pKa = 8.85 VRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 9.67 KK57 pKa = 10.41 KK58 pKa = 10.3 QIAVKK63 pKa = 9.38 QWQPEE68 pKa = 4.47 SITKK72 pKa = 10.26 CKK74 pKa = 10.25 ILGHH78 pKa = 5.77 SALVVGAEE86 pKa = 4.34 GKK88 pKa = 10.5 QMFCYY93 pKa = 7.45 TTNKK97 pKa = 9.88 NIAVPPKK104 pKa = 9.63 SPYY107 pKa = 9.86 GGGFGVEE114 pKa = 4.17 QYY116 pKa = 11.19 SLNYY120 pKa = 10.23 LYY122 pKa = 10.89 EE123 pKa = 4.41 EE124 pKa = 4.54 YY125 pKa = 10.22 TFHH128 pKa = 6.8 NNYY131 pKa = 5.91 WTKK134 pKa = 11.36 SNILKK139 pKa = 10.08 DD140 pKa = 3.35 LCRR143 pKa = 11.84 FLWLKK148 pKa = 10.73 IIFFRR153 pKa = 11.84 DD154 pKa = 2.96 NKK156 pKa = 9.83 TDD158 pKa = 4.52 FIVSYY163 pKa = 10.83 DD164 pKa = 3.64 RR165 pKa = 11.84 NPPFNLTKK173 pKa = 9.64 FTYY176 pKa = 10.04 PGAHH180 pKa = 6.39 PQQMLLQKK188 pKa = 9.62 HH189 pKa = 6.17 HH190 pKa = 6.91 KK191 pKa = 10.16 LILSQMTKK199 pKa = 10.66 NPNKK203 pKa = 9.05 LTKK206 pKa = 9.85 KK207 pKa = 10.45 FKK209 pKa = 10.38 IKK211 pKa = 10.18 PPRR214 pKa = 11.84 QMISKK219 pKa = 9.26 WFFTKK224 pKa = 10.37 PFAKK228 pKa = 10.56 YY229 pKa = 9.72 PLLSLKK235 pKa = 10.68 ASALDD240 pKa = 3.81 LRR242 pKa = 11.84 HH243 pKa = 6.74 SYY245 pKa = 10.5 LGCCNEE251 pKa = 3.61 NSQVYY256 pKa = 8.4 FFYY259 pKa = 10.85 LNHH262 pKa = 6.54 GFYY265 pKa = 10.57 KK266 pKa = 8.23 QTNWGQYY273 pKa = 7.05 STTPYY278 pKa = 10.25 RR279 pKa = 11.84 PSNEE283 pKa = 3.82 INTVYY288 pKa = 11.06 YY289 pKa = 10.32 KK290 pKa = 11.0 DD291 pKa = 3.09 KK292 pKa = 10.8 TGNKK296 pKa = 8.79 KK297 pKa = 10.47 QIGPFNTTATAYY309 pKa = 9.95 DD310 pKa = 3.89 DD311 pKa = 3.83 SVSYY315 pKa = 9.97 TKK317 pKa = 10.68 GYY319 pKa = 8.44 FQPPFLQTQFVCTSSMCDD337 pKa = 3.01 VKK339 pKa = 11.06 AGYY342 pKa = 10.23 LPINVAIYY350 pKa = 11.17 NPMQDD355 pKa = 3.11 SSQGNAIYY363 pKa = 10.29 LVSTLTNTWDD373 pKa = 3.6 QPPKK377 pKa = 10.47 DD378 pKa = 4.08 SSILIQGMPIWLGLFGYY395 pKa = 10.32 LDD397 pKa = 3.72 YY398 pKa = 11.35 VRR400 pKa = 11.84 QVKK403 pKa = 9.87 KK404 pKa = 10.81 DD405 pKa = 3.65 KK406 pKa = 9.98 TWLEE410 pKa = 4.0 SHH412 pKa = 6.26 VLVLKK417 pKa = 10.87 SPAIFTFPQPGADD430 pKa = 2.96 AWYY433 pKa = 9.96 CPLSKK438 pKa = 9.85 TFIDD442 pKa = 4.24 GVGPYY447 pKa = 8.78 RR448 pKa = 11.84 TDD450 pKa = 3.09 LTIKK454 pKa = 10.3 QKK456 pKa = 10.2 EE457 pKa = 3.89 RR458 pKa = 11.84 WFPQIQHH465 pKa = 5.67 QYY467 pKa = 9.72 EE468 pKa = 4.07 ILNSFVEE475 pKa = 4.63 AGPFIPKK482 pKa = 8.38 YY483 pKa = 8.7 TNQSEE488 pKa = 5.07 SNWEE492 pKa = 3.97 LKK494 pKa = 10.18 FKK496 pKa = 11.14 YY497 pKa = 9.58 IFHH500 pKa = 6.81 FKK502 pKa = 9.76 WGGPSLNEE510 pKa = 3.79 PDD512 pKa = 3.13 ITNPANQDD520 pKa = 3.21 QYY522 pKa = 11.93 DD523 pKa = 3.91 VPDD526 pKa = 3.98 TLQQTIQIEE535 pKa = 4.27 NPEE538 pKa = 4.15 RR539 pKa = 11.84 QDD541 pKa = 3.14 PRR543 pKa = 11.84 SILHH547 pKa = 4.78 QWDD550 pKa = 3.39 YY551 pKa = 11.42 RR552 pKa = 11.84 RR553 pKa = 11.84 GFIKK557 pKa = 10.29 EE558 pKa = 3.72 RR559 pKa = 11.84 ALKK562 pKa = 10.64 RR563 pKa = 11.84 MSTYY567 pKa = 10.85 LSTPSDD573 pKa = 3.62 VPEE576 pKa = 4.62 SPEE579 pKa = 3.68 HH580 pKa = 5.31 TPKK583 pKa = 10.39 KK584 pKa = 9.17 KK585 pKa = 10.31 KK586 pKa = 10.13 RR587 pKa = 11.84 VGAQLTLPQEE597 pKa = 4.52 KK598 pKa = 10.39 DD599 pKa = 3.32 QEE601 pKa = 4.52 TLSCLLSLCKK611 pKa = 10.11 EE612 pKa = 4.82 DD613 pKa = 5.7 IYY615 pKa = 11.24 QKK617 pKa = 10.39 PQTEE621 pKa = 4.2 EE622 pKa = 4.32 EE623 pKa = 4.22 INQLIQQQQQQQHH636 pKa = 4.32 QLKK639 pKa = 10.26 RR640 pKa = 11.84 DD641 pKa = 3.45 ILHH644 pKa = 6.82 LMMQIRR650 pKa = 11.84 EE651 pKa = 4.1 QQQLLQLHH659 pKa = 6.16 TGIIPP664 pKa = 3.81
Molecular weight: 79.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.213
IPC2_protein 9.385
IPC_protein 9.341
Toseland 9.911
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.853
Wikipedia 10.335
Rodwell 10.54
Grimsley 10.233
Solomon 10.16
Lehninger 10.116
Nozaki 9.926
DTASelect 9.838
Thurlkill 9.984
EMBOSS 10.321
Sillero 10.072
Patrickios 9.18
IPC_peptide 10.16
IPC2_peptide 8.536
IPC2.peptide.svr19 8.347
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1300
117
664
325.0
37.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.154 ± 1.365
1.692 ± 0.19
5.385 ± 0.966
5.615 ± 1.175
4.385 ± 0.632
4.769 ± 0.472
3.923 ± 1.096
5.462 ± 0.298
8.846 ± 0.474
7.077 ± 0.85
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.584
4.077 ± 0.69
6.154 ± 1.151
6.385 ± 1.17
5.923 ± 0.873
6.692 ± 1.537
8.077 ± 1.103
2.385 ± 0.696
1.846 ± 0.365
3.846 ± 1.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here