Amino acid dipepetide frequency for Variovorax sp. JS1663

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.664AlaAla: 19.664 ± 0.169
1.329AlaCys: 1.329 ± 0.03
6.529AlaAsp: 6.529 ± 0.062
7.299AlaGlu: 7.299 ± 0.063
4.265AlaPhe: 4.265 ± 0.049
11.516AlaGly: 11.516 ± 0.085
2.714AlaHis: 2.714 ± 0.036
5.389AlaIle: 5.389 ± 0.057
3.878AlaLys: 3.878 ± 0.053
15.495AlaLeu: 15.495 ± 0.116
3.651AlaMet: 3.651 ± 0.045
2.746AlaAsn: 2.746 ± 0.041
6.928AlaPro: 6.928 ± 0.083
5.87AlaGln: 5.87 ± 0.063
10.089AlaArg: 10.089 ± 0.089
6.935AlaSer: 6.935 ± 0.062
6.05AlaThr: 6.05 ± 0.059
9.236AlaVal: 9.236 ± 0.079
2.02AlaTrp: 2.02 ± 0.033
2.474AlaTyr: 2.474 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.196CysAla: 1.196 ± 0.027
0.126CysCys: 0.126 ± 0.009
0.53CysAsp: 0.53 ± 0.017
0.579CysGlu: 0.579 ± 0.016
0.32CysPhe: 0.32 ± 0.014
0.96CysGly: 0.96 ± 0.023
0.239CysHis: 0.239 ± 0.011
0.408CysIle: 0.408 ± 0.012
0.228CysLys: 0.228 ± 0.01
0.806CysLeu: 0.806 ± 0.024
0.211CysMet: 0.211 ± 0.011
0.218CysAsn: 0.218 ± 0.009
0.45CysPro: 0.45 ± 0.015
0.238CysGln: 0.238 ± 0.011
0.622CysArg: 0.622 ± 0.017
0.482CysSer: 0.482 ± 0.016
0.497CysThr: 0.497 ± 0.017
0.728CysVal: 0.728 ± 0.02
0.132CysTrp: 0.132 ± 0.008
0.187CysTyr: 0.187 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.553AspAla: 7.553 ± 0.077
0.454AspCys: 0.454 ± 0.015
2.635AspAsp: 2.635 ± 0.035
3.272AspGlu: 3.272 ± 0.043
2.004AspPhe: 2.004 ± 0.029
4.611AspGly: 4.611 ± 0.049
1.044AspHis: 1.044 ± 0.022
2.296AspIle: 2.296 ± 0.035
1.536AspLys: 1.536 ± 0.026
5.236AspLeu: 5.236 ± 0.051
1.173AspMet: 1.173 ± 0.024
1.137AspAsn: 1.137 ± 0.022
3.168AspPro: 3.168 ± 0.037
1.505AspGln: 1.505 ± 0.027
3.577AspArg: 3.577 ± 0.046
2.16AspSer: 2.16 ± 0.028
2.352AspThr: 2.352 ± 0.034
3.814AspVal: 3.814 ± 0.041
0.929AspTrp: 0.929 ± 0.021
1.315AspTyr: 1.315 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.802GluAla: 7.802 ± 0.073
0.401GluCys: 0.401 ± 0.014
2.377GluAsp: 2.377 ± 0.036
2.805GluGlu: 2.805 ± 0.041
1.762GluPhe: 1.762 ± 0.036
4.277GluGly: 4.277 ± 0.051
1.349GluHis: 1.349 ± 0.022
2.683GluIle: 2.683 ± 0.04
1.772GluLys: 1.772 ± 0.035
6.023GluLeu: 6.023 ± 0.064
1.31GluMet: 1.31 ± 0.027
1.202GluAsn: 1.202 ± 0.026
2.709GluPro: 2.709 ± 0.035
2.486GluGln: 2.486 ± 0.042
5.201GluArg: 5.201 ± 0.062
2.629GluSer: 2.629 ± 0.037
2.47GluThr: 2.47 ± 0.037
4.161GluVal: 4.161 ± 0.045
0.748GluTrp: 0.748 ± 0.021
0.969GluTyr: 0.969 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.377PheAla: 4.377 ± 0.043
0.398PheCys: 0.398 ± 0.014
2.522PheAsp: 2.522 ± 0.032
2.175PheGlu: 2.175 ± 0.033
1.326PhePhe: 1.326 ± 0.031
3.465PheGly: 3.465 ± 0.048
0.751PheHis: 0.751 ± 0.021
1.411PheIle: 1.411 ± 0.028
1.086PheLys: 1.086 ± 0.021
2.981PheLeu: 2.981 ± 0.041
0.788PheMet: 0.788 ± 0.019
1.004PheAsn: 1.004 ± 0.025
1.539PhePro: 1.539 ± 0.028
1.046PheGln: 1.046 ± 0.022
2.009PheArg: 2.009 ± 0.029
2.038PheSer: 2.038 ± 0.028
1.797PheThr: 1.797 ± 0.033
2.818PheVal: 2.818 ± 0.035
0.479PheTrp: 0.479 ± 0.016
0.795PheTyr: 0.795 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
9.968GlyAla: 9.968 ± 0.084
0.892GlyCys: 0.892 ± 0.019
3.962GlyAsp: 3.962 ± 0.052
4.765GlyGlu: 4.765 ± 0.047
3.351GlyPhe: 3.351 ± 0.04
7.332GlyGly: 7.332 ± 0.093
2.004GlyHis: 2.004 ± 0.033
4.035GlyIle: 4.035 ± 0.049
3.041GlyLys: 3.041 ± 0.048
8.957GlyLeu: 8.957 ± 0.072
2.309GlyMet: 2.309 ± 0.033
2.058GlyAsn: 2.058 ± 0.033
3.494GlyPro: 3.494 ± 0.043
3.217GlyGln: 3.217 ± 0.043
6.204GlyArg: 6.204 ± 0.054
4.663GlySer: 4.663 ± 0.058
4.65GlyThr: 4.65 ± 0.052
6.417GlyVal: 6.417 ± 0.061
1.471GlyTrp: 1.471 ± 0.028
2.288GlyTyr: 2.288 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
3.008HisAla: 3.008 ± 0.037
0.279HisCys: 0.279 ± 0.011
1.199HisAsp: 1.199 ± 0.024
1.195HisGlu: 1.195 ± 0.028
0.847HisPhe: 0.847 ± 0.019
2.177HisGly: 2.177 ± 0.033
0.602HisHis: 0.602 ± 0.019
0.849HisIle: 0.849 ± 0.02
0.524HisLys: 0.524 ± 0.016
2.177HisLeu: 2.177 ± 0.034
0.483HisMet: 0.483 ± 0.013
0.461HisAsn: 0.461 ± 0.017
1.554HisPro: 1.554 ± 0.026
0.704HisGln: 0.704 ± 0.017
1.609HisArg: 1.609 ± 0.03
0.989HisSer: 0.989 ± 0.023
0.948HisThr: 0.948 ± 0.019
1.673HisVal: 1.673 ± 0.031
0.399HisTrp: 0.399 ± 0.015
0.571HisTyr: 0.571 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.402IleAla: 6.402 ± 0.056
0.392IleCys: 0.392 ± 0.013
3.141IleAsp: 3.141 ± 0.042
3.246IleGlu: 3.246 ± 0.046
1.176IlePhe: 1.176 ± 0.026
4.298IleGly: 4.298 ± 0.049
0.871IleHis: 0.871 ± 0.021
1.288IleIle: 1.288 ± 0.031
1.298IleLys: 1.298 ± 0.029
3.044IleLeu: 3.044 ± 0.037
0.653IleMet: 0.653 ± 0.02
1.231IleAsn: 1.231 ± 0.027
2.023IlePro: 2.023 ± 0.031
1.234IleGln: 1.234 ± 0.025
2.667IleArg: 2.667 ± 0.035
2.123IleSer: 2.123 ± 0.037
2.216IleThr: 2.216 ± 0.037
3.573IleVal: 3.573 ± 0.05
0.449IleTrp: 0.449 ± 0.014
0.935IleTyr: 0.935 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
4.001LysAla: 4.001 ± 0.059
0.148LysCys: 0.148 ± 0.01
1.553LysAsp: 1.553 ± 0.032
1.509LysGlu: 1.509 ± 0.027
0.871LysPhe: 0.871 ± 0.021
2.326LysGly: 2.326 ± 0.037
0.576LysHis: 0.576 ± 0.018
1.319LysIle: 1.319 ± 0.027
1.331LysLys: 1.331 ± 0.035
3.409LysLeu: 3.409 ± 0.045
0.694LysMet: 0.694 ± 0.019
0.818LysAsn: 0.818 ± 0.021
2.064LysPro: 2.064 ± 0.033
1.074LysGln: 1.074 ± 0.023
2.138LysArg: 2.138 ± 0.036
1.641LysSer: 1.641 ± 0.029
1.819LysThr: 1.819 ± 0.032
2.418LysVal: 2.418 ± 0.041
0.36LysTrp: 0.36 ± 0.014
0.611LysTyr: 0.611 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.562LeuAla: 15.562 ± 0.107
1.043LeuCys: 1.043 ± 0.021
5.862LeuAsp: 5.862 ± 0.057
5.327LeuGlu: 5.327 ± 0.065
3.36LeuPhe: 3.36 ± 0.041
8.868LeuGly: 8.868 ± 0.069
2.371LeuHis: 2.371 ± 0.035
4.132LeuIle: 4.132 ± 0.047
3.301LeuLys: 3.301 ± 0.041
11.076LeuLeu: 11.076 ± 0.111
2.493LeuMet: 2.493 ± 0.039
2.414LeuAsn: 2.414 ± 0.036
6.159LeuPro: 6.159 ± 0.059
4.007LeuGln: 4.007 ± 0.045
8.202LeuArg: 8.202 ± 0.075
5.864LeuSer: 5.864 ± 0.048
4.855LeuThr: 4.855 ± 0.052
7.939LeuVal: 7.939 ± 0.063
1.299LeuTrp: 1.299 ± 0.028
1.982LeuTyr: 1.982 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
3.041MetAla: 3.041 ± 0.039
0.153MetCys: 0.153 ± 0.008
1.071MetAsp: 1.071 ± 0.024
1.029MetGlu: 1.029 ± 0.023
0.702MetPhe: 0.702 ± 0.017
1.815MetGly: 1.815 ± 0.032
0.552MetHis: 0.552 ± 0.017
0.885MetIle: 0.885 ± 0.022
1.046MetLys: 1.046 ± 0.023
2.643MetLeu: 2.643 ± 0.035
0.533MetMet: 0.533 ± 0.017
0.852MetAsn: 0.852 ± 0.017
1.613MetPro: 1.613 ± 0.029
1.076MetGln: 1.076 ± 0.022
1.863MetArg: 1.863 ± 0.032
1.595MetSer: 1.595 ± 0.028
1.484MetThr: 1.484 ± 0.028
1.723MetVal: 1.723 ± 0.028
0.205MetTrp: 0.205 ± 0.009
0.368MetTyr: 0.368 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.092AsnAla: 3.092 ± 0.047
0.255AsnCys: 0.255 ± 0.012
1.265AsnAsp: 1.265 ± 0.026
1.189AsnGlu: 1.189 ± 0.025
0.885AsnPhe: 0.885 ± 0.024
2.124AsnGly: 2.124 ± 0.04
0.472AsnHis: 0.472 ± 0.016
1.082AsnIle: 1.082 ± 0.022
0.77AsnLys: 0.77 ± 0.02
2.388AsnLeu: 2.388 ± 0.036
0.538AsnMet: 0.538 ± 0.016
0.663AsnAsn: 0.663 ± 0.017
1.703AsnPro: 1.703 ± 0.036
0.744AsnGln: 0.744 ± 0.019
1.552AsnArg: 1.552 ± 0.026
1.101AsnSer: 1.101 ± 0.026
1.277AsnThr: 1.277 ± 0.024
1.826AsnVal: 1.826 ± 0.035
0.401AsnTrp: 0.401 ± 0.015
0.643AsnTyr: 0.643 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
7.841ProAla: 7.841 ± 0.098
0.359ProCys: 0.359 ± 0.013
3.129ProAsp: 3.129 ± 0.039
3.509ProGlu: 3.509 ± 0.041
1.871ProPhe: 1.871 ± 0.032
5.009ProGly: 5.009 ± 0.06
1.155ProHis: 1.155 ± 0.024
1.958ProIle: 1.958 ± 0.036
1.524ProLys: 1.524 ± 0.027
5.382ProLeu: 5.382 ± 0.052
1.39ProMet: 1.39 ± 0.024
1.249ProAsn: 1.249 ± 0.026
3.058ProPro: 3.058 ± 0.052
2.085ProGln: 2.085 ± 0.032
3.384ProArg: 3.384 ± 0.035
2.916ProSer: 2.916 ± 0.04
2.656ProThr: 2.656 ± 0.039
4.442ProVal: 4.442 ± 0.048
0.853ProTrp: 0.853 ± 0.022
1.196ProTyr: 1.196 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
5.558GlnAla: 5.558 ± 0.057
0.304GlnCys: 0.304 ± 0.012
1.535GlnAsp: 1.535 ± 0.027
1.618GlnGlu: 1.618 ± 0.029
1.199GlnPhe: 1.199 ± 0.025
2.97GlnGly: 2.97 ± 0.039
0.856GlnHis: 0.856 ± 0.019
1.613GlnIle: 1.613 ± 0.028
1.113GlnLys: 1.113 ± 0.025
4.125GlnLeu: 4.125 ± 0.045
0.931GlnMet: 0.931 ± 0.022
0.784GlnAsn: 0.784 ± 0.02
2.291GlnPro: 2.291 ± 0.032
1.838GlnGln: 1.838 ± 0.041
3.446GlnArg: 3.446 ± 0.047
1.857GlnSer: 1.857 ± 0.032
1.686GlnThr: 1.686 ± 0.028
2.891GlnVal: 2.891 ± 0.041
0.592GlnTrp: 0.592 ± 0.016
0.707GlnTyr: 0.707 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
8.896ArgAla: 8.896 ± 0.081
0.66ArgCys: 0.66 ± 0.02
3.718ArgAsp: 3.718 ± 0.045
4.709ArgGlu: 4.709 ± 0.057
3.007ArgPhe: 3.007 ± 0.037
5.085ArgGly: 5.085 ± 0.05
1.984ArgHis: 1.984 ± 0.037
3.751ArgIle: 3.751 ± 0.053
2.091ArgLys: 2.091 ± 0.035
8.294ArgLeu: 8.294 ± 0.08
2.005ArgMet: 2.005 ± 0.029
1.769ArgAsn: 1.769 ± 0.03
3.644ArgPro: 3.644 ± 0.038
2.868ArgGln: 2.868 ± 0.039
5.975ArgArg: 5.975 ± 0.067
3.818ArgSer: 3.818 ± 0.044
3.486ArgThr: 3.486 ± 0.04
5.347ArgVal: 5.347 ± 0.053
1.282ArgTrp: 1.282 ± 0.029
1.812ArgTyr: 1.812 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.466SerAla: 6.466 ± 0.058
0.443SerCys: 0.443 ± 0.014
2.459SerAsp: 2.459 ± 0.035
2.518SerGlu: 2.518 ± 0.036
2.11SerPhe: 2.11 ± 0.034
5.116SerGly: 5.116 ± 0.058
1.155SerHis: 1.155 ± 0.022
2.301SerIle: 2.301 ± 0.038
1.476SerLys: 1.476 ± 0.03
5.413SerLeu: 5.413 ± 0.054
1.364SerMet: 1.364 ± 0.028
1.316SerAsn: 1.316 ± 0.029
2.988SerPro: 2.988 ± 0.039
1.786SerGln: 1.786 ± 0.031
3.588SerArg: 3.588 ± 0.04
2.939SerSer: 2.939 ± 0.038
2.851SerThr: 2.851 ± 0.041
3.812SerVal: 3.812 ± 0.04
0.731SerTrp: 0.731 ± 0.018
1.236SerTyr: 1.236 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
5.832ThrAla: 5.832 ± 0.057
0.391ThrCys: 0.391 ± 0.017
2.395ThrAsp: 2.395 ± 0.031
2.355ThrGlu: 2.355 ± 0.038
1.588ThrPhe: 1.588 ± 0.029
4.559ThrGly: 4.559 ± 0.053
1.11ThrHis: 1.11 ± 0.021
2.016ThrIle: 2.016 ± 0.036
1.191ThrLys: 1.191 ± 0.026
6.007ThrLeu: 6.007 ± 0.059
1.1ThrMet: 1.1 ± 0.024
1.106ThrAsn: 1.106 ± 0.022
3.458ThrPro: 3.458 ± 0.042
1.85ThrGln: 1.85 ± 0.027
3.491ThrArg: 3.491 ± 0.038
2.481ThrSer: 2.481 ± 0.039
2.636ThrThr: 2.636 ± 0.045
4.143ThrVal: 4.143 ± 0.047
0.668ThrTrp: 0.668 ± 0.02
1.009ThrTyr: 1.009 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
9.748ValAla: 9.748 ± 0.083
0.733ValCys: 0.733 ± 0.02
4.068ValAsp: 4.068 ± 0.043
4.284ValGlu: 4.284 ± 0.047
2.706ValPhe: 2.706 ± 0.037
5.614ValGly: 5.614 ± 0.062
1.652ValHis: 1.652 ± 0.025
3.4ValIle: 3.4 ± 0.045
2.342ValLys: 2.342 ± 0.04
8.359ValLeu: 8.359 ± 0.064
1.814ValMet: 1.814 ± 0.029
1.995ValAsn: 1.995 ± 0.033
4.275ValPro: 4.275 ± 0.05
2.807ValGln: 2.807 ± 0.041
5.588ValArg: 5.588 ± 0.053
3.958ValSer: 3.958 ± 0.048
3.802ValThr: 3.802 ± 0.053
6.259ValVal: 6.259 ± 0.073
0.942ValTrp: 0.942 ± 0.022
1.489ValTyr: 1.489 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.428TrpAla: 1.428 ± 0.03
0.156TrpCys: 0.156 ± 0.009
0.624TrpAsp: 0.624 ± 0.017
0.564TrpGlu: 0.564 ± 0.018
0.546TrpPhe: 0.546 ± 0.018
0.979TrpGly: 0.979 ± 0.021
0.37TrpHis: 0.37 ± 0.013
0.713TrpIle: 0.713 ± 0.02
0.474TrpLys: 0.474 ± 0.015
2.024TrpLeu: 2.024 ± 0.037
0.405TrpMet: 0.405 ± 0.013
0.425TrpAsn: 0.425 ± 0.013
0.761TrpPro: 0.761 ± 0.022
0.712TrpGln: 0.712 ± 0.019
1.309TrpArg: 1.309 ± 0.021
0.814TrpSer: 0.814 ± 0.021
0.742TrpThr: 0.742 ± 0.02
0.98TrpVal: 0.98 ± 0.021
0.257TrpTrp: 0.257 ± 0.012
0.318TrpTyr: 0.318 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.641TyrAla: 2.641 ± 0.038
0.24TyrCys: 0.24 ± 0.011
1.198TyrAsp: 1.198 ± 0.032
1.19TyrGlu: 1.19 ± 0.024
0.906TyrPhe: 0.906 ± 0.025
1.978TyrGly: 1.978 ± 0.034
0.42TyrHis: 0.42 ± 0.016
0.731TyrIle: 0.731 ± 0.019
0.66TyrLys: 0.66 ± 0.021
2.275TyrLeu: 2.275 ± 0.034
0.407TyrMet: 0.407 ± 0.011
0.524TyrAsn: 0.524 ± 0.017
1.085TyrPro: 1.085 ± 0.024
0.766TyrGln: 0.766 ± 0.02
1.685TyrArg: 1.685 ± 0.032
1.067TyrSer: 1.067 ± 0.024
1.111TyrThr: 1.111 ± 0.021
1.634TyrVal: 1.634 ± 0.031
0.386TyrTrp: 0.386 ± 0.013
0.574TyrTyr: 0.574 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6556 proteins (2101058 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski