Amino acid dipepetide frequency for Miniimonas sp. PCH200

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.661AlaAla: 21.661 ± 0.215
0.811AlaCys: 0.811 ± 0.025
8.713AlaAsp: 8.713 ± 0.093
8.062AlaGlu: 8.062 ± 0.101
3.599AlaPhe: 3.599 ± 0.059
13.618AlaGly: 13.618 ± 0.16
2.561AlaHis: 2.561 ± 0.053
4.634AlaIle: 4.634 ± 0.071
1.667AlaLys: 1.667 ± 0.051
14.493AlaLeu: 14.493 ± 0.143
2.609AlaMet: 2.609 ± 0.048
1.955AlaAsn: 1.955 ± 0.044
7.936AlaPro: 7.936 ± 0.108
3.673AlaGln: 3.673 ± 0.057
10.225AlaArg: 10.225 ± 0.13
7.506AlaSer: 7.506 ± 0.089
9.214AlaThr: 9.214 ± 0.123
14.129AlaVal: 14.129 ± 0.145
2.217AlaTrp: 2.217 ± 0.046
2.174AlaTyr: 2.174 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.639CysAla: 0.639 ± 0.026
0.054CysCys: 0.054 ± 0.007
0.312CysAsp: 0.312 ± 0.019
0.274CysGlu: 0.274 ± 0.016
0.132CysPhe: 0.132 ± 0.01
0.594CysGly: 0.594 ± 0.024
0.128CysHis: 0.128 ± 0.011
0.14CysIle: 0.14 ± 0.012
0.044CysLys: 0.044 ± 0.006
0.474CysLeu: 0.474 ± 0.02
0.054CysMet: 0.054 ± 0.008
0.078CysAsn: 0.078 ± 0.009
0.335CysPro: 0.335 ± 0.021
0.118CysGln: 0.118 ± 0.01
0.339CysArg: 0.339 ± 0.017
0.35CysSer: 0.35 ± 0.017
0.399CysThr: 0.399 ± 0.026
0.452CysVal: 0.452 ± 0.021
0.077CysTrp: 0.077 ± 0.009
0.096CysTyr: 0.096 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.177AspAla: 9.177 ± 0.1
0.231AspCys: 0.231 ± 0.016
4.389AspAsp: 4.389 ± 0.085
3.65AspGlu: 3.65 ± 0.063
1.335AspPhe: 1.335 ± 0.036
7.578AspGly: 7.578 ± 0.097
1.287AspHis: 1.287 ± 0.034
1.474AspIle: 1.474 ± 0.042
0.61AspLys: 0.61 ± 0.027
7.241AspLeu: 7.241 ± 0.091
0.646AspMet: 0.646 ± 0.024
0.79AspAsn: 0.79 ± 0.033
4.747AspPro: 4.747 ± 0.079
1.316AspGln: 1.316 ± 0.036
4.113AspArg: 4.113 ± 0.065
2.478AspSer: 2.478 ± 0.051
2.746AspThr: 2.746 ± 0.053
7.071AspVal: 7.071 ± 0.091
0.848AspTrp: 0.848 ± 0.027
1.143AspTyr: 1.143 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
7.526GluAla: 7.526 ± 0.112
0.243GluCys: 0.243 ± 0.015
2.909GluAsp: 2.909 ± 0.054
2.928GluGlu: 2.928 ± 0.062
1.276GluPhe: 1.276 ± 0.044
4.279GluGly: 4.279 ± 0.067
1.545GluHis: 1.545 ± 0.039
2.695GluIle: 2.695 ± 0.056
0.901GluLys: 0.901 ± 0.035
6.359GluLeu: 6.359 ± 0.079
0.83GluMet: 0.83 ± 0.032
1.059GluAsn: 1.059 ± 0.038
3.344GluPro: 3.344 ± 0.063
1.986GluGln: 1.986 ± 0.046
5.239GluArg: 5.239 ± 0.085
2.5GluSer: 2.5 ± 0.05
3.05GluThr: 3.05 ± 0.056
5.354GluVal: 5.354 ± 0.075
0.768GluTrp: 0.768 ± 0.023
0.81GluTyr: 0.81 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.486PheAla: 3.486 ± 0.059
0.145PheCys: 0.145 ± 0.011
1.956PheAsp: 1.956 ± 0.043
1.363PheGlu: 1.363 ± 0.037
0.801PhePhe: 0.801 ± 0.031
2.716PheGly: 2.716 ± 0.05
0.521PheHis: 0.521 ± 0.025
0.704PheIle: 0.704 ± 0.025
0.292PheLys: 0.292 ± 0.018
2.424PheLeu: 2.424 ± 0.049
0.316PheMet: 0.316 ± 0.017
0.477PheAsn: 0.477 ± 0.02
1.213PhePro: 1.213 ± 0.035
0.632PheGln: 0.632 ± 0.022
1.578PheArg: 1.578 ± 0.034
1.534PheSer: 1.534 ± 0.042
2.012PheThr: 2.012 ± 0.054
2.468PheVal: 2.468 ± 0.055
0.403PheTrp: 0.403 ± 0.019
0.542PheTyr: 0.542 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
12.193GlyAla: 12.193 ± 0.128
0.514GlyCys: 0.514 ± 0.024
5.581GlyAsp: 5.581 ± 0.082
5.682GlyGlu: 5.682 ± 0.074
2.713GlyPhe: 2.713 ± 0.049
8.837GlyGly: 8.837 ± 0.12
1.92GlyHis: 1.92 ± 0.046
3.556GlyIle: 3.556 ± 0.06
1.51GlyLys: 1.51 ± 0.047
9.187GlyLeu: 9.187 ± 0.097
1.74GlyMet: 1.74 ± 0.043
1.582GlyAsn: 1.582 ± 0.042
4.757GlyPro: 4.757 ± 0.082
2.407GlyGln: 2.407 ± 0.056
7.059GlyArg: 7.059 ± 0.113
5.936GlySer: 5.936 ± 0.085
6.736GlyThr: 6.736 ± 0.115
9.016GlyVal: 9.016 ± 0.105
1.886GlyTrp: 1.886 ± 0.049
2.001GlyTyr: 2.001 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.622HisAla: 2.622 ± 0.053
0.094HisCys: 0.094 ± 0.009
1.447HisAsp: 1.447 ± 0.04
1.125HisGlu: 1.125 ± 0.031
0.395HisPhe: 0.395 ± 0.02
1.959HisGly: 1.959 ± 0.045
0.674HisHis: 0.674 ± 0.031
0.468HisIle: 0.468 ± 0.021
0.2HisLys: 0.2 ± 0.014
2.353HisLeu: 2.353 ± 0.057
0.218HisMet: 0.218 ± 0.014
0.287HisAsn: 0.287 ± 0.016
1.614HisPro: 1.614 ± 0.041
0.496HisGln: 0.496 ± 0.021
1.703HisArg: 1.703 ± 0.046
0.817HisSer: 0.817 ± 0.025
1.026HisThr: 1.026 ± 0.032
1.934HisVal: 1.934 ± 0.048
0.267HisTrp: 0.267 ± 0.015
0.369HisTyr: 0.369 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
5.431IleAla: 5.431 ± 0.088
0.182IleCys: 0.182 ± 0.013
2.686IleAsp: 2.686 ± 0.052
2.044IleGlu: 2.044 ± 0.047
0.802IlePhe: 0.802 ± 0.029
3.55IleGly: 3.55 ± 0.066
0.55IleHis: 0.55 ± 0.021
0.961IleIle: 0.961 ± 0.041
0.487IleLys: 0.487 ± 0.023
2.621IleLeu: 2.621 ± 0.059
0.403IleMet: 0.403 ± 0.02
0.661IleAsn: 0.661 ± 0.025
1.717IlePro: 1.717 ± 0.044
0.633IleGln: 0.633 ± 0.024
1.819IleArg: 1.819 ± 0.044
1.819IleSer: 1.819 ± 0.047
2.431IleThr: 2.431 ± 0.056
3.408IleVal: 3.408 ± 0.063
0.388IleTrp: 0.388 ± 0.02
0.617IleTyr: 0.617 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
1.657LysAla: 1.657 ± 0.052
0.045LysCys: 0.045 ± 0.005
0.767LysAsp: 0.767 ± 0.03
0.69LysGlu: 0.69 ± 0.03
0.296LysPhe: 0.296 ± 0.016
1.015LysGly: 1.015 ± 0.032
0.304LysHis: 0.304 ± 0.018
0.571LysIle: 0.571 ± 0.025
0.41LysLys: 0.41 ± 0.024
1.083LysLeu: 1.083 ± 0.035
0.228LysMet: 0.228 ± 0.014
0.293LysAsn: 0.293 ± 0.016
0.788LysPro: 0.788 ± 0.03
0.425LysGln: 0.425 ± 0.022
0.961LysArg: 0.961 ± 0.041
0.68LysSer: 0.68 ± 0.029
0.776LysThr: 0.776 ± 0.029
1.275LysVal: 1.275 ± 0.039
0.121LysTrp: 0.121 ± 0.011
0.249LysTyr: 0.249 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
16.771LeuAla: 16.771 ± 0.155
0.438LeuCys: 0.438 ± 0.022
6.961LeuAsp: 6.961 ± 0.088
5.554LeuGlu: 5.554 ± 0.089
2.076LeuPhe: 2.076 ± 0.055
9.792LeuGly: 9.792 ± 0.118
1.872LeuHis: 1.872 ± 0.048
2.774LeuIle: 2.774 ± 0.061
1.101LeuLys: 1.101 ± 0.034
10.454LeuLeu: 10.454 ± 0.14
1.302LeuMet: 1.302 ± 0.038
1.428LeuAsn: 1.428 ± 0.039
5.974LeuPro: 5.974 ± 0.069
2.25LeuGln: 2.25 ± 0.04
7.685LeuArg: 7.685 ± 0.092
4.718LeuSer: 4.718 ± 0.067
7.432LeuThr: 7.432 ± 0.098
10.776LeuVal: 10.776 ± 0.123
1.152LeuTrp: 1.152 ± 0.034
1.417LeuTyr: 1.417 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
1.964MetAla: 1.964 ± 0.043
0.096MetCys: 0.096 ± 0.01
0.705MetAsp: 0.705 ± 0.027
0.569MetGlu: 0.569 ± 0.021
0.412MetPhe: 0.412 ± 0.019
1.147MetGly: 1.147 ± 0.037
0.294MetHis: 0.294 ± 0.015
0.597MetIle: 0.597 ± 0.025
0.26MetLys: 0.26 ± 0.014
1.595MetLeu: 1.595 ± 0.032
0.246MetMet: 0.246 ± 0.016
0.312MetAsn: 0.312 ± 0.017
0.932MetPro: 0.932 ± 0.027
0.359MetGln: 0.359 ± 0.016
1.209MetArg: 1.209 ± 0.03
1.224MetSer: 1.224 ± 0.033
1.609MetThr: 1.609 ± 0.037
1.231MetVal: 1.231 ± 0.038
0.187MetTrp: 0.187 ± 0.013
0.213MetTyr: 0.213 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.142AsnAla: 2.142 ± 0.048
0.074AsnCys: 0.074 ± 0.008
1.014AsnAsp: 1.014 ± 0.036
0.786AsnGlu: 0.786 ± 0.028
0.433AsnPhe: 0.433 ± 0.022
1.688AsnGly: 1.688 ± 0.046
0.354AsnHis: 0.354 ± 0.018
0.572AsnIle: 0.572 ± 0.021
0.227AsnLys: 0.227 ± 0.017
1.642AsnLeu: 1.642 ± 0.042
0.217AsnMet: 0.217 ± 0.016
0.398AsnAsn: 0.398 ± 0.021
1.318AsnPro: 1.318 ± 0.038
0.49AsnGln: 0.49 ± 0.022
1.041AsnArg: 1.041 ± 0.03
0.72AsnSer: 0.72 ± 0.027
0.92AsnThr: 0.92 ± 0.037
1.509AsnVal: 1.509 ± 0.041
0.222AsnTrp: 0.222 ± 0.015
0.398AsnTyr: 0.398 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
8.697ProAla: 8.697 ± 0.116
0.207ProCys: 0.207 ± 0.013
4.364ProAsp: 4.364 ± 0.07
3.984ProGlu: 3.984 ± 0.06
1.606ProPhe: 1.606 ± 0.039
6.352ProGly: 6.352 ± 0.098
1.252ProHis: 1.252 ± 0.037
1.607ProIle: 1.607 ± 0.041
0.568ProLys: 0.568 ± 0.03
5.069ProLeu: 5.069 ± 0.072
0.808ProMet: 0.808 ± 0.025
0.821ProAsn: 0.821 ± 0.028
3.408ProPro: 3.408 ± 0.09
1.472ProGln: 1.472 ± 0.038
4.062ProArg: 4.062 ± 0.073
3.699ProSer: 3.699 ± 0.065
4.544ProThr: 4.544 ± 0.09
5.784ProVal: 5.784 ± 0.096
0.993ProTrp: 0.993 ± 0.03
1.033ProTyr: 1.033 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.277GlnAla: 3.277 ± 0.054
0.103GlnCys: 0.103 ± 0.01
1.228GlnAsp: 1.228 ± 0.033
1.302GlnGlu: 1.302 ± 0.036
0.729GlnPhe: 0.729 ± 0.031
2.067GlnGly: 2.067 ± 0.047
0.563GlnHis: 0.563 ± 0.021
1.106GlnIle: 1.106 ± 0.029
0.364GlnLys: 0.364 ± 0.019
2.831GlnLeu: 2.831 ± 0.052
0.389GlnMet: 0.389 ± 0.017
0.426GlnAsn: 0.426 ± 0.02
1.56GlnPro: 1.56 ± 0.043
0.972GlnGln: 0.972 ± 0.035
2.199GlnArg: 2.199 ± 0.041
1.052GlnSer: 1.052 ± 0.031
1.286GlnThr: 1.286 ± 0.034
2.714GlnVal: 2.714 ± 0.057
0.403GlnTrp: 0.403 ± 0.019
0.461GlnTyr: 0.461 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
9.587ArgAla: 9.587 ± 0.13
0.416ArgCys: 0.416 ± 0.02
4.337ArgAsp: 4.337 ± 0.078
4.483ArgGlu: 4.483 ± 0.073
1.952ArgPhe: 1.952 ± 0.047
6.017ArgGly: 6.017 ± 0.089
1.636ArgHis: 1.636 ± 0.039
2.788ArgIle: 2.788 ± 0.053
0.961ArgLys: 0.961 ± 0.034
7.622ArgLeu: 7.622 ± 0.123
1.402ArgMet: 1.402 ± 0.04
1.073ArgAsn: 1.073 ± 0.033
4.476ArgPro: 4.476 ± 0.072
1.815ArgGln: 1.815 ± 0.039
7.856ArgArg: 7.856 ± 0.143
4.464ArgSer: 4.464 ± 0.071
5.063ArgThr: 5.063 ± 0.067
6.023ArgVal: 6.023 ± 0.082
1.375ArgTrp: 1.375 ± 0.038
1.549ArgTyr: 1.549 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.944SerAla: 6.944 ± 0.086
0.332SerCys: 0.332 ± 0.021
3.243SerAsp: 3.243 ± 0.053
2.572SerGlu: 2.572 ± 0.057
1.691SerPhe: 1.691 ± 0.038
5.898SerGly: 5.898 ± 0.08
1.002SerHis: 1.002 ± 0.03
1.943SerIle: 1.943 ± 0.041
0.634SerLys: 0.634 ± 0.027
5.132SerLeu: 5.132 ± 0.073
0.997SerMet: 0.997 ± 0.029
0.907SerAsn: 0.907 ± 0.029
3.624SerPro: 3.624 ± 0.059
1.376SerGln: 1.376 ± 0.038
3.952SerArg: 3.952 ± 0.065
3.811SerSer: 3.811 ± 0.075
3.954SerThr: 3.954 ± 0.064
4.524SerVal: 4.524 ± 0.059
0.979SerTrp: 0.979 ± 0.03
1.178SerTyr: 1.178 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
9.227ThrAla: 9.227 ± 0.133
0.37ThrCys: 0.37 ± 0.022
3.962ThrAsp: 3.962 ± 0.062
3.108ThrGlu: 3.108 ± 0.053
2.196ThrPhe: 2.196 ± 0.053
6.566ThrGly: 6.566 ± 0.088
1.185ThrHis: 1.185 ± 0.036
2.514ThrIle: 2.514 ± 0.052
0.795ThrLys: 0.795 ± 0.029
6.502ThrLeu: 6.502 ± 0.075
0.917ThrMet: 0.917 ± 0.029
1.221ThrAsn: 1.221 ± 0.045
4.954ThrPro: 4.954 ± 0.093
1.539ThrGln: 1.539 ± 0.04
4.036ThrArg: 4.036 ± 0.072
4.409ThrSer: 4.409 ± 0.073
5.432ThrThr: 5.432 ± 0.106
6.499ThrVal: 6.499 ± 0.137
1.216ThrTrp: 1.216 ± 0.037
1.536ThrTyr: 1.536 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
14.555ValAla: 14.555 ± 0.138
0.489ValCys: 0.489 ± 0.021
6.161ValAsp: 6.161 ± 0.093
5.693ValGlu: 5.693 ± 0.074
2.078ValPhe: 2.078 ± 0.04
8.307ValGly: 8.307 ± 0.097
1.824ValHis: 1.824 ± 0.043
3.099ValIle: 3.099 ± 0.058
1.151ValLys: 1.151 ± 0.035
11.079ValLeu: 11.079 ± 0.132
1.395ValMet: 1.395 ± 0.032
1.611ValAsn: 1.611 ± 0.044
5.879ValPro: 5.879 ± 0.082
2.076ValGln: 2.076 ± 0.043
6.936ValArg: 6.936 ± 0.099
4.85ValSer: 4.85 ± 0.069
7.297ValThr: 7.297 ± 0.145
11.863ValVal: 11.863 ± 0.124
1.239ValTrp: 1.239 ± 0.034
1.378ValTyr: 1.378 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.67TrpAla: 1.67 ± 0.041
0.119TrpCys: 0.119 ± 0.009
0.938TrpAsp: 0.938 ± 0.029
0.753TrpGlu: 0.753 ± 0.029
0.522TrpPhe: 0.522 ± 0.023
1.106TrpGly: 1.106 ± 0.037
0.304TrpHis: 0.304 ± 0.017
0.607TrpIle: 0.607 ± 0.023
0.21TrpLys: 0.21 ± 0.014
1.777TrpLeu: 1.777 ± 0.048
0.267TrpMet: 0.267 ± 0.014
0.407TrpAsn: 0.407 ± 0.02
0.767TrpPro: 0.767 ± 0.029
0.508TrpGln: 0.508 ± 0.023
1.342TrpArg: 1.342 ± 0.034
1.068TrpSer: 1.068 ± 0.037
1.088TrpThr: 1.088 ± 0.033
1.306TrpVal: 1.306 ± 0.038
0.428TrpTrp: 0.428 ± 0.024
0.289TrpTyr: 0.289 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.233TyrAla: 2.233 ± 0.049
0.125TyrCys: 0.125 ± 0.011
1.197TyrAsp: 1.197 ± 0.036
0.98TyrGlu: 0.98 ± 0.03
0.54TyrPhe: 0.54 ± 0.024
1.723TyrGly: 1.723 ± 0.042
0.295TyrHis: 0.295 ± 0.016
0.399TyrIle: 0.399 ± 0.019
0.242TyrLys: 0.242 ± 0.015
1.987TyrLeu: 1.987 ± 0.044
0.178TyrMet: 0.178 ± 0.012
0.378TyrAsn: 0.378 ± 0.019
0.985TyrPro: 0.985 ± 0.035
0.489TyrGln: 0.489 ± 0.023
1.543TyrArg: 1.543 ± 0.042
1.06TyrSer: 1.06 ± 0.031
1.168TyrThr: 1.168 ± 0.043
1.622TyrVal: 1.622 ± 0.039
0.31TyrTrp: 0.31 ± 0.018
0.399TyrTyr: 0.399 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3302 proteins (1140190 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski