Desulfallas thermosapovorans DSM 6562
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2860 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S4ZSM6|A0A5S4ZSM6_9FIRM Probable molybdenum cofactor guanylyltransferase OS=Desulfallas thermosapovorans DSM 6562 OX=1121431 GN=mobA PE=3 SV=1
MM1 pKa = 7.34 IRR3 pKa = 11.84 NHH5 pKa = 6.62 KK6 pKa = 10.5 GGIFVSDD13 pKa = 4.53 LKK15 pKa = 11.47 SKK17 pKa = 10.2 VAYY20 pKa = 10.01 LQGLANGMNMEE31 pKa = 4.87 ADD33 pKa = 3.61 SKK35 pKa = 10.84 EE36 pKa = 3.86 GKK38 pKa = 10.15 LLGGIIDD45 pKa = 4.11 VLDD48 pKa = 3.96 EE49 pKa = 4.4 FADD52 pKa = 3.75 SFSEE56 pKa = 4.35 LEE58 pKa = 4.01 EE59 pKa = 4.1 SQEE62 pKa = 3.79 QLEE65 pKa = 4.84 EE66 pKa = 4.11 YY67 pKa = 11.02 VEE69 pKa = 4.2 TLDD72 pKa = 3.62 EE73 pKa = 5.13 DD74 pKa = 5.54 LYY76 pKa = 10.81 TLEE79 pKa = 4.58 NDD81 pKa = 3.19 MHH83 pKa = 8.08 GEE85 pKa = 4.12 EE86 pKa = 4.21 EE87 pKa = 4.33 LEE89 pKa = 4.06 EE90 pKa = 4.16 YY91 pKa = 10.38 MEE93 pKa = 4.4 VDD95 pKa = 4.0 CPRR98 pKa = 11.84 CGEE101 pKa = 4.3 TVLFDD106 pKa = 4.16 SDD108 pKa = 3.47 IVEE111 pKa = 4.7 DD112 pKa = 3.89 EE113 pKa = 5.12 DD114 pKa = 5.05 IIEE117 pKa = 4.37 VTCPNCDD124 pKa = 2.5 EE125 pKa = 4.37 VVFVNDD131 pKa = 4.87 DD132 pKa = 3.69 GYY134 pKa = 10.24 EE135 pKa = 4.08 TADD138 pKa = 3.62 EE139 pKa = 4.33 PEE141 pKa = 4.3 ILHH144 pKa = 6.14 SRR146 pKa = 11.84 NSAIEE151 pKa = 3.72 EE152 pKa = 4.59 DD153 pKa = 3.6 II154 pKa = 4.74
Molecular weight: 17.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.528
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.541
Sillero 3.783
Patrickios 1.799
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A5S4ZQB2|A0A5S4ZQB2_9FIRM Amino-acid N-acetyltransferase OS=Desulfallas thermosapovorans DSM 6562 OX=1121431 GN=LX24_02000 PE=4 SV=1
MM1 pKa = 7.5 SSKK4 pKa = 10.58 EE5 pKa = 3.87 CIIRR9 pKa = 11.84 VDD11 pKa = 3.27 IPTGRR16 pKa = 11.84 VLEE19 pKa = 4.9 DD20 pKa = 3.18 NLHH23 pKa = 6.28 AFWEE27 pKa = 4.72 TQARR31 pKa = 11.84 RR32 pKa = 11.84 SDD34 pKa = 3.65 LPDD37 pKa = 4.0 PVWHH41 pKa = 6.96 SNPAMHH47 pKa = 7.22 GYY49 pKa = 10.39 AGPQPRR55 pKa = 11.84 GAQTGTVQPPYY66 pKa = 10.42 EE67 pKa = 4.01 LLEE70 pKa = 4.03 RR71 pKa = 11.84 VQRR74 pKa = 11.84 FNAARR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 TKK84 pKa = 10.61 NAWTDD89 pKa = 3.14 TEE91 pKa = 4.37 MYY93 pKa = 10.26 RR94 pKa = 11.84 LAVLVAVQNQKK105 pKa = 9.27 PHH107 pKa = 4.96 QAAARR112 pKa = 11.84 LARR115 pKa = 11.84 TPRR118 pKa = 11.84 ACRR121 pKa = 11.84 DD122 pKa = 3.22 MFRR125 pKa = 11.84 TLKK128 pKa = 10.11 NAGVII133 pKa = 3.9
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.589
IPC_protein 10.628
Toseland 10.672
ProMoST 10.511
Dawson 10.774
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.804
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.54
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.716
Patrickios 10.54
IPC_peptide 10.95
IPC2_peptide 9.78
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2860
0
2860
839009
40
1766
293.4
32.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.8 ± 0.052
1.228 ± 0.021
5.086 ± 0.031
6.563 ± 0.043
3.605 ± 0.032
8.145 ± 0.044
1.918 ± 0.018
6.723 ± 0.046
5.468 ± 0.038
10.067 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.749 ± 0.019
3.98 ± 0.026
4.391 ± 0.028
3.575 ± 0.029
5.764 ± 0.045
4.845 ± 0.032
5.069 ± 0.027
7.875 ± 0.044
0.947 ± 0.018
3.202 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here