CRESS virus sp. ctczB4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 8.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9F2|A0A5Q2W9F2_9VIRU Putative capsid protein OS=CRESS virus sp. ctczB4 OX=2656682 PE=4 SV=1
MM1 pKa = 7.64SNGQGIFWLLTIPHH15 pKa = 6.42YY16 pKa = 11.16GFTPYY21 pKa = 10.6LPPGCSWIKK30 pKa = 10.04GQLEE34 pKa = 3.92RR35 pKa = 11.84GEE37 pKa = 4.73GGFLHH42 pKa = 6.08WQIIVAFQSRR52 pKa = 11.84CRR54 pKa = 11.84LRR56 pKa = 11.84GVKK59 pKa = 10.02QIFGSEE65 pKa = 3.99AHH67 pKa = 6.61CEE69 pKa = 4.07LSRR72 pKa = 11.84SAAANEE78 pKa = 4.32YY79 pKa = 8.84VWKK82 pKa = 9.72EE83 pKa = 3.75QTRR86 pKa = 11.84VEE88 pKa = 4.18GTPFEE93 pKa = 5.35LGAKK97 pKa = 9.26PIRR100 pKa = 11.84RR101 pKa = 11.84NSQTDD106 pKa = 3.33WEE108 pKa = 4.98SVWTAAKK115 pKa = 10.57SRR117 pKa = 11.84DD118 pKa = 3.61LEE120 pKa = 4.64AIPAHH125 pKa = 5.35TRR127 pKa = 11.84VLCFRR132 pKa = 11.84SILSIASYY140 pKa = 10.78FDD142 pKa = 3.0TAIAVEE148 pKa = 4.52RR149 pKa = 11.84SVQVYY154 pKa = 8.52WGRR157 pKa = 11.84TGTGKK162 pKa = 10.03SRR164 pKa = 11.84RR165 pKa = 11.84AWEE168 pKa = 3.92AAGVDD173 pKa = 4.54AYY175 pKa = 11.64SKK177 pKa = 10.73DD178 pKa = 3.51PRR180 pKa = 11.84SKK182 pKa = 9.96FWFGYY187 pKa = 9.13RR188 pKa = 11.84GQEE191 pKa = 3.24RR192 pKa = 11.84VVIDD196 pKa = 3.8EE197 pKa = 4.04FRR199 pKa = 11.84GGIDD203 pKa = 3.25VSHH206 pKa = 7.54LLRR209 pKa = 11.84WLDD212 pKa = 3.64RR213 pKa = 11.84YY214 pKa = 9.75PVLVEE219 pKa = 4.22LKK221 pKa = 10.42GSSVPLAARR230 pKa = 11.84EE231 pKa = 3.63IWITSNVDD239 pKa = 2.94PRR241 pKa = 11.84NWYY244 pKa = 8.99PDD246 pKa = 3.32LDD248 pKa = 3.99EE249 pKa = 4.5EE250 pKa = 4.83TRR252 pKa = 11.84CALIRR257 pKa = 11.84RR258 pKa = 11.84LSITHH263 pKa = 6.54FNN265 pKa = 3.55

Molecular weight: 30.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W884|A0A5Q2W884_9VIRU ATP-dependent helicase Rep OS=CRESS virus sp. ctczB4 OX=2656682 PE=3 SV=1
MM1 pKa = 7.85IEE3 pKa = 4.61GSQQGKK9 pKa = 8.99VLQAMLLLTRR19 pKa = 11.84RR20 pKa = 11.84IQLLIVLFLLEE31 pKa = 3.69VLYY34 pKa = 10.56INKK37 pKa = 9.3SHH39 pKa = 6.54SSKK42 pKa = 9.74TGRR45 pKa = 11.84TISRR49 pKa = 11.84EE50 pKa = 3.78NEE52 pKa = 3.68LRR54 pKa = 11.84SS55 pKa = 3.48

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

960

55

640

320.0

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.604 ± 1.157

1.146 ± 0.352

3.646 ± 0.709

5.104 ± 0.937

3.854 ± 0.444

8.438 ± 0.68

1.146 ± 0.536

5.833 ± 0.748

5.0 ± 0.688

6.875 ± 2.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.78

4.792 ± 1.181

4.271 ± 0.831

4.688 ± 0.673

7.292 ± 1.235

8.438 ± 0.492

6.563 ± 0.805

7.187 ± 0.535

1.979 ± 1.097

3.958 ± 0.538
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski