Prevotella maculosa OT 289

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella maculosa

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2568 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1HN39|H1HN39_9BACT HTH araC/xylS-type domain-containing protein OS=Prevotella maculosa OT 289 OX=999422 GN=HMPREF9944_01583 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.77NISIFSIEE10 pKa = 4.03GVDD13 pKa = 4.41ARR15 pKa = 11.84EE16 pKa = 3.8ICNAQLGDD24 pKa = 4.33ALFAPCSVCDD34 pKa = 3.24KK35 pKa = 10.24WKK37 pKa = 11.15GNFPSVDD44 pKa = 3.27NLFYY48 pKa = 10.78GYY50 pKa = 10.75VEE52 pKa = 5.43DD53 pKa = 6.28DD54 pKa = 2.84IFYY57 pKa = 10.7NYY59 pKa = 10.56SDD61 pKa = 5.21DD62 pKa = 3.95EE63 pKa = 4.92FEE65 pKa = 4.27THH67 pKa = 5.47VNAMFDD73 pKa = 3.22

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1HJE2|H1HJE2_9BACT OMP_b-brl_3 domain-containing protein OS=Prevotella maculosa OT 289 OX=999422 GN=HMPREF9944_00286 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2568

0

2568

911703

21

2483

355.0

40.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.563 ± 0.047

1.303 ± 0.019

5.678 ± 0.037

5.935 ± 0.05

4.522 ± 0.03

6.954 ± 0.038

2.214 ± 0.022

6.34 ± 0.044

6.503 ± 0.038

9.038 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.023

4.978 ± 0.038

3.752 ± 0.024

3.547 ± 0.025

5.182 ± 0.041

5.805 ± 0.037

5.74 ± 0.033

6.465 ± 0.031

1.3 ± 0.02

4.344 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski