Aeromonas phage phiAS7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Aerosvirus; Aeromonas virus AS7

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6UK25|H6UK25_9CAUD Uncharacterized protein OS=Aeromonas phage phiAS7 OX=1141132 GN=phiAS7_00018 PE=4 SV=1
MM1 pKa = 7.79HH2 pKa = 7.57IMTDD6 pKa = 3.6VLVTAAALAAAINPLDD22 pKa = 4.87DD23 pKa = 5.36SPLLDD28 pKa = 3.79VWDD31 pKa = 3.98QGCVEE36 pKa = 5.34LYY38 pKa = 10.44HH39 pKa = 7.15QIGEE43 pKa = 4.18WAGVIDD49 pKa = 4.78KK50 pKa = 10.85IEE52 pKa = 4.3AKK54 pKa = 10.53VSEE57 pKa = 4.7LVEE60 pKa = 4.18KK61 pKa = 10.79HH62 pKa = 6.6DD63 pKa = 4.42GDD65 pKa = 3.84WPGVFAYY72 pKa = 9.47EE73 pKa = 4.0VTEE76 pKa = 4.1NVGTAIRR83 pKa = 11.84AAILEE88 pKa = 4.3GKK90 pKa = 9.95GDD92 pKa = 4.21EE93 pKa = 5.29ISEE96 pKa = 4.09QWVQSAALALAVKK109 pKa = 10.09FCGEE113 pKa = 3.98CPVIKK118 pKa = 10.42EE119 pKa = 3.87EE120 pKa = 4.53LLAYY124 pKa = 10.37GNN126 pKa = 3.96

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6UK09|H6UK09_9CAUD Putative DNA polymerase OS=Aeromonas phage phiAS7 OX=1141132 GN=phiAS7_00002 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84VITKK6 pKa = 10.23AEE8 pKa = 3.97KK9 pKa = 10.11AVQKK13 pKa = 10.76ARR15 pKa = 11.84RR16 pKa = 11.84LWSAMWKK23 pKa = 10.17RR24 pKa = 11.84LNKK27 pKa = 9.65WGDD30 pKa = 3.35GYY32 pKa = 10.39EE33 pKa = 4.09RR34 pKa = 11.84KK35 pKa = 10.08HH36 pKa = 5.01IARR39 pKa = 11.84RR40 pKa = 11.84AVVVLIASPFVLLEE54 pKa = 3.92MGRR57 pKa = 11.84QAYY60 pKa = 9.2HH61 pKa = 6.78AVAEE65 pKa = 4.69CAAEE69 pKa = 4.48LYY71 pKa = 10.55QYY73 pKa = 10.34IRR75 pKa = 11.84HH76 pKa = 5.3NQQ78 pKa = 2.99

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12657

45

1249

248.2

27.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.737 ± 0.652

0.956 ± 0.15

5.926 ± 0.178

6.85 ± 0.312

3.389 ± 0.156

7.458 ± 0.285

2.149 ± 0.184

4.582 ± 0.243

6.265 ± 0.309

8.343 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.397 ± 0.165

3.848 ± 0.202

3.792 ± 0.232

4.567 ± 0.292

5.736 ± 0.21

5.436 ± 0.237

5.12 ± 0.268

6.613 ± 0.233

1.58 ± 0.127

3.255 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski