Amino acid dipepetide frequency for Bacillus phage MG-B1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.39AlaAla: 0.39 ± 0.204
0.39AlaCys: 0.39 ± 0.199
2.988AlaAsp: 2.988 ± 0.55
2.078AlaGlu: 2.078 ± 0.641
2.338AlaPhe: 2.338 ± 0.524
3.248AlaGly: 3.248 ± 0.96
0.26AlaHis: 0.26 ± 0.174
2.728AlaIle: 2.728 ± 0.54
4.287AlaLys: 4.287 ± 0.847
2.728AlaLeu: 2.728 ± 0.461
1.819AlaMet: 1.819 ± 0.488
2.078AlaAsn: 2.078 ± 0.471
1.429AlaPro: 1.429 ± 0.431
1.169AlaGln: 1.169 ± 0.375
1.039AlaArg: 1.039 ± 0.312
2.728AlaSer: 2.728 ± 0.421
2.208AlaThr: 2.208 ± 0.586
2.338AlaVal: 2.338 ± 0.769
0.65AlaTrp: 0.65 ± 0.293
1.689AlaTyr: 1.689 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.52CysAla: 0.52 ± 0.337
0.26CysCys: 0.26 ± 0.199
0.909CysAsp: 0.909 ± 0.405
0.779CysGlu: 0.779 ± 0.33
0.39CysPhe: 0.39 ± 0.224
1.689CysGly: 1.689 ± 0.446
0.0CysHis: 0.0 ± 0.0
0.909CysIle: 0.909 ± 0.327
0.909CysLys: 0.909 ± 0.322
0.65CysLeu: 0.65 ± 0.264
0.39CysMet: 0.39 ± 0.224
0.52CysAsn: 0.52 ± 0.232
0.65CysPro: 0.65 ± 0.279
0.26CysGln: 0.26 ± 0.173
0.13CysArg: 0.13 ± 0.139
0.909CysSer: 0.909 ± 0.307
0.39CysThr: 0.39 ± 0.225
1.039CysVal: 1.039 ± 0.393
0.13CysTrp: 0.13 ± 0.133
0.909CysTyr: 0.909 ± 0.329
0.0CysXaa: 0.0 ± 0.0
Asp
2.338AspAla: 2.338 ± 0.477
0.779AspCys: 0.779 ± 0.348
4.417AspAsp: 4.417 ± 1.049
4.157AspGlu: 4.157 ± 0.859
2.728AspPhe: 2.728 ± 0.545
5.066AspGly: 5.066 ± 1.465
0.52AspHis: 0.52 ± 0.26
3.897AspIle: 3.897 ± 0.641
4.936AspLys: 4.936 ± 1.023
4.287AspLeu: 4.287 ± 0.828
1.559AspMet: 1.559 ± 0.436
3.767AspAsn: 3.767 ± 0.656
2.728AspPro: 2.728 ± 0.523
2.338AspGln: 2.338 ± 0.519
2.208AspArg: 2.208 ± 0.518
2.988AspSer: 2.988 ± 0.644
3.118AspThr: 3.118 ± 0.547
4.287AspVal: 4.287 ± 0.837
1.039AspTrp: 1.039 ± 0.312
3.767AspTyr: 3.767 ± 0.688
0.0AspXaa: 0.0 ± 0.0
Glu
3.248GluAla: 3.248 ± 0.591
1.039GluCys: 1.039 ± 0.396
2.988GluAsp: 2.988 ± 0.585
7.534GluGlu: 7.534 ± 1.75
3.637GluPhe: 3.637 ± 0.913
4.936GluGly: 4.936 ± 0.62
0.909GluHis: 0.909 ± 0.306
6.235GluIle: 6.235 ± 0.987
5.456GluLys: 5.456 ± 1.248
8.833GluLeu: 8.833 ± 1.203
3.118GluMet: 3.118 ± 0.685
4.936GluAsn: 4.936 ± 0.759
1.429GluPro: 1.429 ± 0.505
1.949GluGln: 1.949 ± 0.674
3.637GluArg: 3.637 ± 0.787
3.897GluSer: 3.897 ± 0.646
3.767GluThr: 3.767 ± 0.654
4.417GluVal: 4.417 ± 0.587
1.039GluTrp: 1.039 ± 0.346
3.118GluTyr: 3.118 ± 0.662
0.0GluXaa: 0.0 ± 0.0
Phe
1.429PheAla: 1.429 ± 0.396
0.26PheCys: 0.26 ± 0.18
2.858PheAsp: 2.858 ± 0.594
3.507PheGlu: 3.507 ± 0.824
1.429PhePhe: 1.429 ± 0.439
1.819PheGly: 1.819 ± 0.428
1.689PheHis: 1.689 ± 0.474
4.417PheIle: 4.417 ± 0.696
4.806PheLys: 4.806 ± 0.997
2.468PheLeu: 2.468 ± 0.659
1.429PheMet: 1.429 ± 0.445
3.378PheAsn: 3.378 ± 0.835
1.169PhePro: 1.169 ± 0.429
1.429PheGln: 1.429 ± 0.426
1.169PheArg: 1.169 ± 0.488
2.338PheSer: 2.338 ± 0.546
3.637PheThr: 3.637 ± 0.523
3.507PheVal: 3.507 ± 0.771
0.13PheTrp: 0.13 ± 0.117
1.949PheTyr: 1.949 ± 0.483
0.0PheXaa: 0.0 ± 0.0
Gly
1.429GlyAla: 1.429 ± 0.664
0.39GlyCys: 0.39 ± 0.191
3.637GlyAsp: 3.637 ± 0.908
5.196GlyGlu: 5.196 ± 0.719
3.767GlyPhe: 3.767 ± 0.583
3.118GlyGly: 3.118 ± 0.865
0.909GlyHis: 0.909 ± 0.277
4.027GlyIle: 4.027 ± 0.728
7.924GlyLys: 7.924 ± 0.918
5.586GlyLeu: 5.586 ± 0.787
1.559GlyMet: 1.559 ± 0.477
5.066GlyAsn: 5.066 ± 0.906
0.13GlyPro: 0.13 ± 0.127
1.689GlyGln: 1.689 ± 0.373
1.689GlyArg: 1.689 ± 0.497
4.677GlySer: 4.677 ± 1.059
5.196GlyThr: 5.196 ± 1.172
4.157GlyVal: 4.157 ± 0.717
0.52GlyTrp: 0.52 ± 0.259
3.637GlyTyr: 3.637 ± 0.808
0.0GlyXaa: 0.0 ± 0.0
His
0.39HisAla: 0.39 ± 0.199
0.26HisCys: 0.26 ± 0.152
1.299HisAsp: 1.299 ± 0.361
1.169HisGlu: 1.169 ± 0.508
0.779HisPhe: 0.779 ± 0.261
0.65HisGly: 0.65 ± 0.235
0.52HisHis: 0.52 ± 0.232
1.169HisIle: 1.169 ± 0.349
1.169HisLys: 1.169 ± 0.322
1.819HisLeu: 1.819 ± 0.413
0.65HisMet: 0.65 ± 0.308
1.169HisAsn: 1.169 ± 0.359
0.26HisPro: 0.26 ± 0.195
0.65HisGln: 0.65 ± 0.247
0.65HisArg: 0.65 ± 0.25
1.169HisSer: 1.169 ± 0.298
0.909HisThr: 0.909 ± 0.365
1.559HisVal: 1.559 ± 0.509
0.13HisTrp: 0.13 ± 0.123
1.039HisTyr: 1.039 ± 0.345
0.0HisXaa: 0.0 ± 0.0
Ile
2.078IleAla: 2.078 ± 0.463
0.909IleCys: 0.909 ± 0.459
5.846IleAsp: 5.846 ± 0.94
5.716IleGlu: 5.716 ± 1.001
1.299IlePhe: 1.299 ± 0.403
5.716IleGly: 5.716 ± 0.861
2.598IleHis: 2.598 ± 0.492
4.157IleIle: 4.157 ± 0.806
6.625IleLys: 6.625 ± 0.923
3.378IleLeu: 3.378 ± 0.812
2.468IleMet: 2.468 ± 0.506
4.806IleAsn: 4.806 ± 0.877
2.078IlePro: 2.078 ± 0.465
3.248IleGln: 3.248 ± 0.434
3.897IleArg: 3.897 ± 0.69
4.677IleSer: 4.677 ± 0.866
4.677IleThr: 4.677 ± 0.617
3.378IleVal: 3.378 ± 0.492
0.779IleTrp: 0.779 ± 0.386
2.988IleTyr: 2.988 ± 0.663
0.0IleXaa: 0.0 ± 0.0
Lys
3.897LysAla: 3.897 ± 0.636
1.429LysCys: 1.429 ± 0.388
5.846LysAsp: 5.846 ± 0.998
9.093LysGlu: 9.093 ± 1.791
3.507LysPhe: 3.507 ± 0.843
6.105LysGly: 6.105 ± 0.864
2.208LysHis: 2.208 ± 0.496
5.456LysIle: 5.456 ± 0.769
7.145LysLys: 7.145 ± 1.064
5.976LysLeu: 5.976 ± 0.88
4.027LysMet: 4.027 ± 0.639
5.716LysAsn: 5.716 ± 0.905
2.338LysPro: 2.338 ± 0.616
3.248LysGln: 3.248 ± 0.639
4.287LysArg: 4.287 ± 0.764
4.027LysSer: 4.027 ± 0.738
6.235LysThr: 6.235 ± 1.341
5.326LysVal: 5.326 ± 0.921
0.909LysTrp: 0.909 ± 0.237
5.326LysTyr: 5.326 ± 0.85
0.0LysXaa: 0.0 ± 0.0
Leu
2.858LeuAla: 2.858 ± 0.463
0.52LeuCys: 0.52 ± 0.311
4.806LeuAsp: 4.806 ± 0.798
5.586LeuGlu: 5.586 ± 1.023
2.858LeuPhe: 2.858 ± 0.765
3.897LeuGly: 3.897 ± 0.795
1.429LeuHis: 1.429 ± 0.415
5.586LeuIle: 5.586 ± 0.839
8.704LeuLys: 8.704 ± 1.837
5.066LeuLeu: 5.066 ± 0.829
2.338LeuMet: 2.338 ± 0.529
5.716LeuAsn: 5.716 ± 0.855
2.598LeuPro: 2.598 ± 0.579
3.248LeuGln: 3.248 ± 0.669
3.507LeuArg: 3.507 ± 0.608
4.936LeuSer: 4.936 ± 1.049
4.287LeuThr: 4.287 ± 0.722
3.897LeuVal: 3.897 ± 0.855
1.039LeuTrp: 1.039 ± 0.34
2.988LeuTyr: 2.988 ± 0.6
0.0LeuXaa: 0.0 ± 0.0
Met
1.039MetAla: 1.039 ± 0.323
0.65MetCys: 0.65 ± 0.225
1.299MetAsp: 1.299 ± 0.353
1.819MetGlu: 1.819 ± 0.595
2.468MetPhe: 2.468 ± 0.631
2.468MetGly: 2.468 ± 0.615
0.26MetHis: 0.26 ± 0.179
2.988MetIle: 2.988 ± 0.662
2.468MetLys: 2.468 ± 0.538
2.858MetLeu: 2.858 ± 0.515
1.299MetMet: 1.299 ± 0.47
3.248MetAsn: 3.248 ± 0.717
0.52MetPro: 0.52 ± 0.218
0.779MetGln: 0.779 ± 0.332
1.819MetArg: 1.819 ± 0.414
1.689MetSer: 1.689 ± 0.296
1.689MetThr: 1.689 ± 0.542
1.559MetVal: 1.559 ± 0.414
0.65MetTrp: 0.65 ± 0.204
1.299MetTyr: 1.299 ± 0.368
0.0MetXaa: 0.0 ± 0.0
Asn
3.378AsnAla: 3.378 ± 0.786
0.65AsnCys: 0.65 ± 0.314
4.806AsnAsp: 4.806 ± 0.835
5.846AsnGlu: 5.846 ± 0.937
3.378AsnPhe: 3.378 ± 0.635
4.417AsnGly: 4.417 ± 0.718
0.779AsnHis: 0.779 ± 0.286
5.196AsnIle: 5.196 ± 0.979
5.586AsnLys: 5.586 ± 1.05
5.196AsnLeu: 5.196 ± 0.898
1.559AsnMet: 1.559 ± 0.453
4.287AsnAsn: 4.287 ± 0.946
2.468AsnPro: 2.468 ± 0.612
2.858AsnGln: 2.858 ± 0.82
2.988AsnArg: 2.988 ± 0.855
4.027AsnSer: 4.027 ± 0.618
4.677AsnThr: 4.677 ± 0.979
4.806AsnVal: 4.806 ± 0.952
0.65AsnTrp: 0.65 ± 0.223
2.468AsnTyr: 2.468 ± 0.614
0.0AsnXaa: 0.0 ± 0.0
Pro
0.65ProAla: 0.65 ± 0.262
0.779ProCys: 0.779 ± 0.294
1.299ProAsp: 1.299 ± 0.309
1.819ProGlu: 1.819 ± 0.492
1.689ProPhe: 1.689 ± 0.445
0.39ProGly: 0.39 ± 0.183
0.13ProHis: 0.13 ± 0.139
1.819ProIle: 1.819 ± 0.603
2.598ProLys: 2.598 ± 0.563
2.598ProLeu: 2.598 ± 0.578
0.909ProMet: 0.909 ± 0.465
2.078ProAsn: 2.078 ± 0.524
1.039ProPro: 1.039 ± 0.333
0.779ProGln: 0.779 ± 0.231
0.39ProArg: 0.39 ± 0.225
2.338ProSer: 2.338 ± 0.591
2.208ProThr: 2.208 ± 0.529
2.208ProVal: 2.208 ± 0.483
0.26ProTrp: 0.26 ± 0.162
1.169ProTyr: 1.169 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
1.299GlnAla: 1.299 ± 0.336
0.26GlnCys: 0.26 ± 0.189
1.169GlnAsp: 1.169 ± 0.252
1.819GlnGlu: 1.819 ± 0.576
1.559GlnPhe: 1.559 ± 0.487
2.338GlnGly: 2.338 ± 1.006
0.26GlnHis: 0.26 ± 0.175
2.728GlnIle: 2.728 ± 0.624
3.507GlnLys: 3.507 ± 0.668
3.507GlnLeu: 3.507 ± 0.67
0.52GlnMet: 0.52 ± 0.281
2.338GlnAsn: 2.338 ± 0.473
1.559GlnPro: 1.559 ± 0.44
1.819GlnGln: 1.819 ± 0.694
1.689GlnArg: 1.689 ± 0.523
1.429GlnSer: 1.429 ± 0.33
2.208GlnThr: 2.208 ± 0.636
1.689GlnVal: 1.689 ± 0.494
0.65GlnTrp: 0.65 ± 0.33
2.078GlnTyr: 2.078 ± 0.508
0.0GlnXaa: 0.0 ± 0.0
Arg
2.078ArgAla: 2.078 ± 0.49
0.52ArgCys: 0.52 ± 0.254
2.598ArgAsp: 2.598 ± 0.626
3.378ArgGlu: 3.378 ± 0.543
1.949ArgPhe: 1.949 ± 0.624
1.949ArgGly: 1.949 ± 0.472
0.52ArgHis: 0.52 ± 0.25
1.689ArgIle: 1.689 ± 0.476
5.066ArgLys: 5.066 ± 0.966
2.468ArgLeu: 2.468 ± 0.53
1.429ArgMet: 1.429 ± 0.401
2.988ArgAsn: 2.988 ± 0.699
1.169ArgPro: 1.169 ± 0.354
1.949ArgGln: 1.949 ± 0.382
1.559ArgArg: 1.559 ± 0.374
1.689ArgSer: 1.689 ± 0.418
2.728ArgThr: 2.728 ± 0.541
1.819ArgVal: 1.819 ± 0.625
0.26ArgTrp: 0.26 ± 0.152
1.949ArgTyr: 1.949 ± 0.622
0.0ArgXaa: 0.0 ± 0.0
Ser
2.728SerAla: 2.728 ± 0.782
1.169SerCys: 1.169 ± 0.409
3.897SerAsp: 3.897 ± 0.545
3.507SerGlu: 3.507 ± 0.736
3.378SerPhe: 3.378 ± 0.914
4.027SerGly: 4.027 ± 0.881
1.039SerHis: 1.039 ± 0.492
5.326SerIle: 5.326 ± 0.902
4.287SerLys: 4.287 ± 0.689
4.677SerLeu: 4.677 ± 0.923
2.468SerMet: 2.468 ± 0.413
4.287SerAsn: 4.287 ± 0.904
0.909SerPro: 0.909 ± 0.357
2.078SerGln: 2.078 ± 0.491
2.208SerArg: 2.208 ± 0.495
2.988SerSer: 2.988 ± 0.66
2.858SerThr: 2.858 ± 0.784
3.767SerVal: 3.767 ± 0.628
0.39SerTrp: 0.39 ± 0.185
2.468SerTyr: 2.468 ± 0.614
0.0SerXaa: 0.0 ± 0.0
Thr
2.208ThrAla: 2.208 ± 0.711
0.65ThrCys: 0.65 ± 0.289
2.988ThrAsp: 2.988 ± 0.594
3.897ThrGlu: 3.897 ± 0.522
2.728ThrPhe: 2.728 ± 0.494
5.326ThrGly: 5.326 ± 0.829
1.169ThrHis: 1.169 ± 0.355
4.677ThrIle: 4.677 ± 0.595
5.586ThrLys: 5.586 ± 0.718
4.547ThrLeu: 4.547 ± 0.707
1.299ThrMet: 1.299 ± 0.289
4.547ThrAsn: 4.547 ± 1.001
1.689ThrPro: 1.689 ± 0.468
2.078ThrGln: 2.078 ± 0.67
3.118ThrArg: 3.118 ± 0.751
3.248ThrSer: 3.248 ± 0.71
3.767ThrThr: 3.767 ± 0.999
5.456ThrVal: 5.456 ± 0.839
0.65ThrTrp: 0.65 ± 0.276
2.208ThrTyr: 2.208 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
3.637ValAla: 3.637 ± 0.777
0.39ValCys: 0.39 ± 0.223
3.637ValAsp: 3.637 ± 0.615
5.196ValGlu: 5.196 ± 0.884
1.819ValPhe: 1.819 ± 0.511
3.118ValGly: 3.118 ± 0.582
0.909ValHis: 0.909 ± 0.307
5.326ValIle: 5.326 ± 0.677
5.846ValLys: 5.846 ± 0.909
3.897ValLeu: 3.897 ± 0.885
1.559ValMet: 1.559 ± 0.431
3.248ValAsn: 3.248 ± 0.839
1.949ValPro: 1.949 ± 0.425
1.559ValGln: 1.559 ± 0.463
2.858ValArg: 2.858 ± 0.442
5.586ValSer: 5.586 ± 0.886
4.287ValThr: 4.287 ± 0.997
4.417ValVal: 4.417 ± 0.628
1.299ValTrp: 1.299 ± 0.421
2.858ValTyr: 2.858 ± 0.541
0.0ValXaa: 0.0 ± 0.0
Trp
0.52TrpAla: 0.52 ± 0.206
0.13TrpCys: 0.13 ± 0.131
0.26TrpAsp: 0.26 ± 0.263
1.299TrpGlu: 1.299 ± 0.41
1.299TrpPhe: 1.299 ± 0.386
1.039TrpGly: 1.039 ± 0.344
0.52TrpHis: 0.52 ± 0.2
0.39TrpIle: 0.39 ± 0.212
0.909TrpLys: 0.909 ± 0.245
1.299TrpLeu: 1.299 ± 0.402
0.52TrpMet: 0.52 ± 0.242
1.689TrpAsn: 1.689 ± 0.47
0.0TrpPro: 0.0 ± 0.0
0.39TrpGln: 0.39 ± 0.201
0.13TrpArg: 0.13 ± 0.102
0.39TrpSer: 0.39 ± 0.199
0.52TrpThr: 0.52 ± 0.224
0.65TrpVal: 0.65 ± 0.287
0.13TrpTrp: 0.13 ± 0.132
0.13TrpTyr: 0.13 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.468TyrAla: 2.468 ± 0.657
0.909TyrCys: 0.909 ± 0.291
3.378TyrAsp: 3.378 ± 0.532
2.728TyrGlu: 2.728 ± 0.759
1.819TyrPhe: 1.819 ± 0.355
2.988TyrGly: 2.988 ± 0.513
0.779TyrHis: 0.779 ± 0.298
2.728TyrIle: 2.728 ± 0.696
4.287TyrLys: 4.287 ± 0.811
4.027TyrLeu: 4.027 ± 0.648
1.819TyrMet: 1.819 ± 0.619
4.157TyrAsn: 4.157 ± 0.554
1.039TyrPro: 1.039 ± 0.362
0.909TyrGln: 0.909 ± 0.346
0.779TyrArg: 0.779 ± 0.282
2.728TyrSer: 2.728 ± 0.708
2.468TyrThr: 2.468 ± 0.511
3.118TyrVal: 3.118 ± 0.7
0.909TyrTrp: 0.909 ± 0.328
2.078TyrTyr: 2.078 ± 0.601
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 42 proteins (7699 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski