Firmicutes bacterium CAG:424

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6R442|R6R442_9FIRM ATPases of the AAA+ class OS=Firmicutes bacterium CAG:424 OX=1263022 GN=BN652_00445 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84KK3 pKa = 7.9KK4 pKa = 8.85TAAIVTGFMITLGMLGGCGSAGDD27 pKa = 3.93STGNANASGEE37 pKa = 3.95NHH39 pKa = 6.95LNFGCYY45 pKa = 9.3VYY47 pKa = 10.05STSFDD52 pKa = 3.28PAAYY56 pKa = 10.06QNASWQGMRR65 pKa = 11.84WGITEE70 pKa = 3.76TLYY73 pKa = 11.01RR74 pKa = 11.84FNDD77 pKa = 3.77DD78 pKa = 3.13STIRR82 pKa = 11.84PWLSDD87 pKa = 3.31SYY89 pKa = 11.2EE90 pKa = 3.83VSEE93 pKa = 5.18DD94 pKa = 3.07HH95 pKa = 6.43KK96 pKa = 9.8TWTFHH101 pKa = 6.39IRR103 pKa = 11.84DD104 pKa = 3.53GVKK107 pKa = 10.22FSNGSPCDD115 pKa = 3.61AQAVADD121 pKa = 3.78SMNRR125 pKa = 11.84LFEE128 pKa = 4.35VCNSTEE134 pKa = 3.97YY135 pKa = 11.07SSTPRR140 pKa = 11.84QYY142 pKa = 10.95IDD144 pKa = 3.28MASIEE149 pKa = 4.74ADD151 pKa = 3.31PEE153 pKa = 4.5ANTVTIVTNTAYY165 pKa = 10.62ADD167 pKa = 3.64LRR169 pKa = 11.84GPLCFPFYY177 pKa = 10.51TIIDD181 pKa = 3.91VEE183 pKa = 4.44GDD185 pKa = 3.35LTDD188 pKa = 4.11AEE190 pKa = 4.74HH191 pKa = 6.96PGGYY195 pKa = 5.03TTSVIGTGPYY205 pKa = 10.4VLDD208 pKa = 4.55SFDD211 pKa = 4.19EE212 pKa = 4.32LSKK215 pKa = 11.17SGEE218 pKa = 3.97LVKK221 pKa = 11.1NEE223 pKa = 4.21NYY225 pKa = 9.96WNGDD229 pKa = 3.12VPYY232 pKa = 10.58DD233 pKa = 3.73SVTMMFIEE241 pKa = 5.78DD242 pKa = 3.95DD243 pKa = 3.73TTKK246 pKa = 11.32AMAIEE251 pKa = 4.65SGDD254 pKa = 3.24IDD256 pKa = 3.87LTEE259 pKa = 4.9NITTISDD266 pKa = 3.72LQKK269 pKa = 11.17LEE271 pKa = 4.35GDD273 pKa = 3.51DD274 pKa = 3.65AFYY277 pKa = 11.14VSKK280 pKa = 9.66KK281 pKa = 8.12TGVRR285 pKa = 11.84TGFALVNFDD294 pKa = 5.22GILGNDD300 pKa = 4.02TLRR303 pKa = 11.84QAVFMAIDD311 pKa = 4.02GQTLCDD317 pKa = 3.55VTVGGMYY324 pKa = 10.23NYY326 pKa = 10.2EE327 pKa = 4.41PGVLPDD333 pKa = 3.46TLAYY337 pKa = 10.43DD338 pKa = 3.63SEE340 pKa = 4.84KK341 pKa = 10.85LVNPYY346 pKa = 10.03TYY348 pKa = 10.21DD349 pKa = 3.26QEE351 pKa = 4.93AAIQLLDD358 pKa = 4.07DD359 pKa = 3.98NGIRR363 pKa = 11.84DD364 pKa = 3.75TDD366 pKa = 3.42GDD368 pKa = 4.38GIRR371 pKa = 11.84EE372 pKa = 3.9LDD374 pKa = 3.57GEE376 pKa = 4.51NIVLEE381 pKa = 3.81FDD383 pKa = 3.61TYY385 pKa = 11.57KK386 pKa = 10.69NRR388 pKa = 11.84CLSDD392 pKa = 3.47FAEE395 pKa = 5.98GIQSQLAAIGIGCNVNVLDD414 pKa = 6.28SDD416 pKa = 4.4TLWMEE421 pKa = 4.18MQEE424 pKa = 4.21GDD426 pKa = 3.68YY427 pKa = 11.32DD428 pKa = 4.16LSNSNWITVGTGDD441 pKa = 4.85PEE443 pKa = 4.79AFLLNWYY450 pKa = 10.26GDD452 pKa = 3.26GGEE455 pKa = 4.39NFFTEE460 pKa = 4.79DD461 pKa = 3.06NTDD464 pKa = 2.85ATNYY468 pKa = 9.44CRR470 pKa = 11.84YY471 pKa = 10.76DD472 pKa = 3.38NEE474 pKa = 4.72EE475 pKa = 3.92YY476 pKa = 11.02NKK478 pKa = 10.61LYY480 pKa = 10.58EE481 pKa = 3.99QFKK484 pKa = 10.85ASMDD488 pKa = 3.47EE489 pKa = 4.16EE490 pKa = 4.19EE491 pKa = 5.41RR492 pKa = 11.84KK493 pKa = 10.63DD494 pKa = 4.54LVIQMEE500 pKa = 4.14QVLIDD505 pKa = 3.75DD506 pKa = 4.6CAVLVHH512 pKa = 6.61GYY514 pKa = 10.14YY515 pKa = 10.43NSTMISNKK523 pKa = 9.62EE524 pKa = 3.8KK525 pKa = 10.69VAGAEE530 pKa = 3.95IPAFDD535 pKa = 4.73YY536 pKa = 11.24YY537 pKa = 10.71WLTTDD542 pKa = 4.56IKK544 pKa = 10.57PAEE547 pKa = 4.04

Molecular weight: 60.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6RNI8|R6RNI8_9FIRM Abhydrolase_3 domain-containing protein OS=Firmicutes bacterium CAG:424 OX=1263022 GN=BN652_01611 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2835

0

2835

903456

29

1505

318.7

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.085 ± 0.045

1.551 ± 0.02

4.976 ± 0.035

8.381 ± 0.054

4.289 ± 0.033

7.151 ± 0.049

1.804 ± 0.022

7.092 ± 0.041

7.144 ± 0.039

9.387 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.247 ± 0.021

4.076 ± 0.029

3.351 ± 0.028

3.688 ± 0.032

4.201 ± 0.032

5.458 ± 0.031

5.182 ± 0.028

6.803 ± 0.037

0.986 ± 0.017

4.147 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski