Cognatiyoonia koreensis
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3582 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I0N387|A0A1I0N387_9RHOB Uncharacterized protein OS=Cognatiyoonia koreensis OX=364200 GN=SAMN04488515_0391 PE=4 SV=1
MM1 pKa = 7.19 PTIDD5 pKa = 3.68 VTDD8 pKa = 3.85 IAANFTMSFGYY19 pKa = 10.21 QFSSYY24 pKa = 10.02 VGSGSTFYY32 pKa = 10.81 SWEE35 pKa = 4.19 TNGGHH40 pKa = 7.08 TITLTGTGITTGVGNLPTGGTITGMLIDD68 pKa = 5.05 LNGTAPSDD76 pKa = 3.51 VDD78 pKa = 3.84 VEE80 pKa = 4.11 ISGIEE85 pKa = 3.97 NKK87 pKa = 10.53 NITDD91 pKa = 3.47 IVFVAGPDD99 pKa = 3.65 SEE101 pKa = 4.42 NTLWATVLDD110 pKa = 4.01 GDD112 pKa = 4.35 VTVDD116 pKa = 3.9 GAPTEE121 pKa = 4.1 DD122 pKa = 3.86 FRR124 pKa = 11.84 VEE126 pKa = 3.74 INTDD130 pKa = 3.57 FDD132 pKa = 3.87 EE133 pKa = 4.94 VSFGIHH139 pKa = 5.98 NGEE142 pKa = 4.46 DD143 pKa = 2.98 ATIRR147 pKa = 11.84 GDD149 pKa = 3.87 FFGFTYY155 pKa = 10.56 FGGVQVLEE163 pKa = 4.61 GTAHH167 pKa = 6.58 FSAGEE172 pKa = 3.85 TDD174 pKa = 4.59 FEE176 pKa = 4.94 GFIYY180 pKa = 9.97 WFNPAQTVRR189 pKa = 11.84 GNARR193 pKa = 11.84 LLGGDD198 pKa = 3.95 DD199 pKa = 3.86 TIIWTEE205 pKa = 3.93 DD206 pKa = 2.98 TRR208 pKa = 11.84 SYY210 pKa = 10.24 PSSYY214 pKa = 10.61 VYY216 pKa = 10.57 AYY218 pKa = 10.45 ADD220 pKa = 3.28 ASFVSGSAVVVGGDD234 pKa = 3.76 DD235 pKa = 5.03 LLDD238 pKa = 3.75 ARR240 pKa = 11.84 NAGDD244 pKa = 4.13 GIGAFLVGDD253 pKa = 4.66 AYY255 pKa = 10.28 HH256 pKa = 5.93 TTAATAMVIGGDD268 pKa = 4.37 DD269 pKa = 3.34 ILFGANASDD278 pKa = 3.72 GAPTNRR284 pKa = 11.84 LYY286 pKa = 11.53 GDD288 pKa = 3.58 VVVNFTDD295 pKa = 3.52 TLIAGDD301 pKa = 3.56 DD302 pKa = 4.04 TIYY305 pKa = 11.19 GRR307 pKa = 11.84 AGNDD311 pKa = 3.26 EE312 pKa = 4.47 IYY314 pKa = 10.88 GDD316 pKa = 3.89 TGSTGTNYY324 pKa = 9.94 IGGDD328 pKa = 3.45 DD329 pKa = 5.21 LIYY332 pKa = 10.79 GGSDD336 pKa = 3.9 GEE338 pKa = 4.11 DD339 pKa = 2.99 LMYY342 pKa = 10.77 GGRR345 pKa = 11.84 GNDD348 pKa = 2.63 RR349 pKa = 11.84 MYY351 pKa = 10.69 GGKK354 pKa = 10.46 GIVEE358 pKa = 3.94 MRR360 pKa = 11.84 GDD362 pKa = 3.47 QDD364 pKa = 3.49 NDD366 pKa = 3.03 KK367 pKa = 10.84 LYY369 pKa = 11.04 AGDD372 pKa = 4.04 GTTTAYY378 pKa = 10.36 GGSGDD383 pKa = 5.14 DD384 pKa = 4.38 LIKK387 pKa = 10.51 VTGDD391 pKa = 3.59 GEE393 pKa = 4.62 GSFYY397 pKa = 10.84 GGSGIDD403 pKa = 3.27 RR404 pKa = 11.84 ISYY407 pKa = 9.77 YY408 pKa = 10.46 DD409 pKa = 3.56 AEE411 pKa = 4.63 GGITIDD417 pKa = 4.51 LGANTISRR425 pKa = 11.84 YY426 pKa = 8.71 WADD429 pKa = 4.94 DD430 pKa = 3.37 DD431 pKa = 4.82 TISNFEE437 pKa = 4.15 NVSGSKK443 pKa = 10.23 VGNNKK448 pKa = 9.17 IFGTSGANDD457 pKa = 3.28 IDD459 pKa = 4.27 TYY461 pKa = 11.48 DD462 pKa = 4.35 GNDD465 pKa = 3.06 KK466 pKa = 11.11 VYY468 pKa = 11.06 GGGGGDD474 pKa = 4.29 KK475 pKa = 10.53 ISLGKK480 pKa = 10.88 GNDD483 pKa = 3.36 YY484 pKa = 11.24 VKK486 pKa = 11.14 ADD488 pKa = 4.64 FGANTYY494 pKa = 9.7 EE495 pKa = 4.44 GSSGLDD501 pKa = 3.03 TISYY505 pKa = 9.69 YY506 pKa = 11.05 NSNDD510 pKa = 3.11 GVIVDD515 pKa = 4.74 LKK517 pKa = 10.96 HH518 pKa = 6.39 NEE520 pKa = 4.0 AEE522 pKa = 4.17 GGYY525 pKa = 10.79 AEE527 pKa = 5.58 GDD529 pKa = 3.7 TISGFEE535 pKa = 4.19 RR536 pKa = 11.84 LTGSTEE542 pKa = 3.77 DD543 pKa = 3.63 DD544 pKa = 3.49 KK545 pKa = 11.89 LYY547 pKa = 11.16 GSDD550 pKa = 4.12 GGNILKK556 pKa = 10.52 GGSGEE561 pKa = 3.99 DD562 pKa = 3.8 SLWGRR567 pKa = 11.84 DD568 pKa = 4.05 GNDD571 pKa = 3.07 KK572 pKa = 11.09 LYY574 pKa = 11.13 GGSNSDD580 pKa = 3.46 RR581 pKa = 11.84 FDD583 pKa = 3.63 GGEE586 pKa = 4.38 GNDD589 pKa = 3.7 KK590 pKa = 10.91 LYY592 pKa = 11.14 GGSGVDD598 pKa = 3.11 TFHH601 pKa = 7.45 FDD603 pKa = 4.17 KK604 pKa = 11.3 GDD606 pKa = 3.95 DD607 pKa = 3.8 FDD609 pKa = 4.83 TVMDD613 pKa = 4.23 FQNNADD619 pKa = 4.43 RR620 pKa = 11.84 IEE622 pKa = 4.45 IDD624 pKa = 4.06 GYY626 pKa = 11.52 SSGFDD631 pKa = 2.99 AFDD634 pKa = 3.57 YY635 pKa = 8.49 ATQVGTDD642 pKa = 3.2 VVFNFGGGDD651 pKa = 3.58 GLIVEE656 pKa = 4.81 NVLIGQLGNDD666 pKa = 3.88 LFVVV670 pKa = 4.15
Molecular weight: 70.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.516
EMBOSS 3.681
Sillero 3.834
Patrickios 1.507
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A1I0S0D7|A0A1I0S0D7_9RHOB S-adenosylmethionine uptake transporter OS=Cognatiyoonia koreensis OX=364200 GN=SAMN04488515_3650 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.53 ILNNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.47 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3582
0
3582
1123027
31
4083
313.5
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.705 ± 0.057
0.911 ± 0.013
6.572 ± 0.049
5.505 ± 0.036
3.95 ± 0.029
8.579 ± 0.054
2.007 ± 0.024
5.631 ± 0.032
3.454 ± 0.037
9.55 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.838 ± 0.026
3.001 ± 0.029
4.874 ± 0.033
3.288 ± 0.024
5.952 ± 0.037
5.192 ± 0.028
5.95 ± 0.046
7.347 ± 0.033
1.376 ± 0.019
2.318 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here