Horseradish latent virus
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5J1S3|Q5J1S3_9VIRU Protein 3 OS=Horseradish latent virus OX=264076 PE=3 SV=1
MM1 pKa = 7.6 PRR3 pKa = 11.84 TVGQTIDD10 pKa = 5.02 DD11 pKa = 4.01 FWSNLGVNQLDD22 pKa = 3.57 EE23 pKa = 5.06 HH24 pKa = 6.57 EE25 pKa = 4.72 FDD27 pKa = 5.43 LMINLMTDD35 pKa = 3.52 DD36 pKa = 5.29 DD37 pKa = 4.74 VSSDD41 pKa = 3.35 TSFDD45 pKa = 3.53 SLTSLFSEE53 pKa = 4.72 LLQVEE58 pKa = 4.18 QEE60 pKa = 4.02 ITSEE64 pKa = 4.09 EE65 pKa = 4.3 EE66 pKa = 3.72 RR67 pKa = 11.84 CQLLEE72 pKa = 4.96 KK73 pKa = 10.21 EE74 pKa = 4.54 DD75 pKa = 4.37 SSDD78 pKa = 3.25 EE79 pKa = 4.48 SIPNDD84 pKa = 3.34 SDD86 pKa = 3.65 EE87 pKa = 4.5 EE88 pKa = 4.48 SVPEE92 pKa = 3.85 QVRR95 pKa = 11.84 MEE97 pKa = 4.26 KK98 pKa = 10.75 GKK100 pKa = 11.12 GPAKK104 pKa = 10.24 PYY106 pKa = 10.65 DD107 pKa = 3.69 YY108 pKa = 10.89 FDD110 pKa = 4.44 NEE112 pKa = 4.37 SSDD115 pKa = 3.75 MEE117 pKa = 4.4 YY118 pKa = 11.37 GDD120 pKa = 3.98 YY121 pKa = 11.13 NPYY124 pKa = 10.02 GRR126 pKa = 11.84 SGQTNKK132 pKa = 9.93 PARR135 pKa = 11.84 TRR137 pKa = 11.84 PKK139 pKa = 10.21 LASEE143 pKa = 4.1 TLGRR147 pKa = 11.84 NPRR150 pKa = 11.84 ILNLDD155 pKa = 3.64 CTNSLRR161 pKa = 11.84 DD162 pKa = 3.36 RR163 pKa = 11.84 KK164 pKa = 10.38 RR165 pKa = 11.84 LVEE168 pKa = 3.85 EE169 pKa = 4.12 WGAEE173 pKa = 3.52 ISLTIQTNLDD183 pKa = 3.83 EE184 pKa = 5.12 YY185 pKa = 11.1 SDD187 pKa = 4.16 PDD189 pKa = 3.6 MVLLLMEE196 pKa = 5.22 HH197 pKa = 6.42 LTAGSVKK204 pKa = 10.66 SFIKK208 pKa = 6.67 TTKK211 pKa = 9.21 WSEE214 pKa = 3.94 LSGGIYY220 pKa = 10.23 DD221 pKa = 4.44 LVMEE225 pKa = 6.0 GIHH228 pKa = 7.11 VMFLGEE234 pKa = 4.06 QPNEE238 pKa = 4.04 AFDD241 pKa = 3.79 KK242 pKa = 11.12 AKK244 pKa = 10.29 EE245 pKa = 3.63 QAAAKK250 pKa = 10.23 DD251 pKa = 3.91 KK252 pKa = 10.58 LLKK255 pKa = 9.68 MQLCDD260 pKa = 3.12 ICSLDD265 pKa = 3.39 TFTCAFEE272 pKa = 3.96 KK273 pKa = 10.77 ALYY276 pKa = 10.25 KK277 pKa = 10.3 LDD279 pKa = 4.4 SGDD282 pKa = 3.73 FPPIIEE288 pKa = 4.39 QYY290 pKa = 9.43 LAKK293 pKa = 10.17 IPEE296 pKa = 4.34 VGAKK300 pKa = 9.21 AQQRR304 pKa = 11.84 YY305 pKa = 9.03 KK306 pKa = 11.06 EE307 pKa = 4.13 EE308 pKa = 3.72 ATGAMKK314 pKa = 10.83 YY315 pKa = 10.76 SLGFANKK322 pKa = 9.23 IVKK325 pKa = 10.23 EE326 pKa = 3.93 EE327 pKa = 3.96 LKK329 pKa = 10.6 KK330 pKa = 10.55 VCEE333 pKa = 4.03 LTRR336 pKa = 11.84 LQKK339 pKa = 10.53 KK340 pKa = 9.31 LKK342 pKa = 10.06 KK343 pKa = 9.95 FRR345 pKa = 11.84 KK346 pKa = 6.93 TCCKK350 pKa = 9.74 QVEE353 pKa = 4.15 EE354 pKa = 4.15 RR355 pKa = 11.84 LEE357 pKa = 4.1 YY358 pKa = 10.59 GCRR361 pKa = 11.84 PIYY364 pKa = 9.89 KK365 pKa = 9.64 KK366 pKa = 10.17 KK367 pKa = 10.16 SKK369 pKa = 9.87 RR370 pKa = 11.84 RR371 pKa = 11.84 KK372 pKa = 8.48 PKK374 pKa = 8.02 VRR376 pKa = 11.84 KK377 pKa = 7.87 YY378 pKa = 10.95 KK379 pKa = 10.16 KK380 pKa = 8.97 YY381 pKa = 10.74 KK382 pKa = 8.28 PFKK385 pKa = 9.51 RR386 pKa = 11.84 RR387 pKa = 11.84 KK388 pKa = 8.41 KK389 pKa = 10.35 FKK391 pKa = 7.66 TGKK394 pKa = 8.91 YY395 pKa = 9.12 FRR397 pKa = 11.84 PKK399 pKa = 9.62 TEE401 pKa = 4.31 KK402 pKa = 10.41 KK403 pKa = 10.04 VCPKK407 pKa = 10.94 GKK409 pKa = 10.36 GKK411 pKa = 9.66 NCKK414 pKa = 9.48 CWICNVEE421 pKa = 3.58 GHH423 pKa = 6.09 YY424 pKa = 11.32 ANEE427 pKa = 4.32 CPNRR431 pKa = 11.84 QTSQKK436 pKa = 10.95 AHH438 pKa = 6.98 ILQEE442 pKa = 4.56 AIQLGLQPVEE452 pKa = 4.2 DD453 pKa = 4.23 VYY455 pKa = 11.42 EE456 pKa = 4.67 GEE458 pKa = 4.1 QEE460 pKa = 4.3 VFVIEE465 pKa = 4.26 YY466 pKa = 10.22 IEE468 pKa = 4.09 VSEE471 pKa = 5.27 DD472 pKa = 3.36 EE473 pKa = 4.57 TSTSEE478 pKa = 4.98 DD479 pKa = 3.73 DD480 pKa = 3.79 GTSTSEE486 pKa = 5.44 DD487 pKa = 3.49 SDD489 pKa = 3.85 SDD491 pKa = 3.58
Molecular weight: 56.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.193
IPC2_protein 5.296
IPC_protein 5.232
Toseland 5.143
ProMoST 5.296
Dawson 5.194
Bjellqvist 5.334
Wikipedia 5.054
Rodwell 5.118
Grimsley 5.067
Solomon 5.194
Lehninger 5.156
Nozaki 5.308
DTASelect 5.436
Thurlkill 5.143
EMBOSS 5.092
Sillero 5.385
Patrickios 4.202
IPC_peptide 5.207
IPC2_peptide 5.385
IPC2.peptide.svr19 5.387
Protein with the highest isoelectric point:
>tr|K0DKX7|K0DKX7_9VIRU Uncharacterized protein OS=Horseradish latent virus OX=264076 PE=4 SV=1
MM1 pKa = 7.84 KK2 pKa = 10.41 DD3 pKa = 3.23 NEE5 pKa = 4.52 KK6 pKa = 10.02 TGSSDD11 pKa = 3.37 GHH13 pKa = 6.82 CSGQIYY19 pKa = 10.81 LNLLILSLRR28 pKa = 11.84 RR29 pKa = 11.84 LTLISGSKK37 pKa = 10.4 GLLINPYY44 pKa = 7.57 QFKK47 pKa = 10.6 ILLIKK52 pKa = 10.23 KK53 pKa = 8.28 LNKK56 pKa = 8.75 WFKK59 pKa = 9.05 CHH61 pKa = 6.8 KK62 pKa = 8.93 STFRR66 pKa = 11.84 STNLVVQKK74 pKa = 10.6 LVSEE78 pKa = 4.52 PP79 pKa = 3.46
Molecular weight: 9.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.098
IPC2_protein 9.37
IPC_protein 9.297
Toseland 10.321
ProMoST 9.823
Dawson 10.423
Bjellqvist 10.014
Wikipedia 10.526
Rodwell 11.169
Grimsley 10.452
Solomon 10.452
Lehninger 10.438
Nozaki 10.321
DTASelect 9.999
Thurlkill 10.306
EMBOSS 10.687
Sillero 10.335
Patrickios 10.95
IPC_peptide 10.467
IPC2_peptide 8.639
IPC2.peptide.svr19 8.325
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2435
60
679
304.4
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.6 ± 0.489
1.889 ± 0.255
5.298 ± 0.459
7.885 ± 0.719
3.901 ± 0.493
4.969 ± 0.19
1.725 ± 0.365
7.351 ± 0.72
10.554 ± 0.551
8.665 ± 0.647
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.177 ± 0.194
5.421 ± 0.623
4.6 ± 0.519
4.23 ± 0.493
4.312 ± 0.409
7.598 ± 0.717
5.955 ± 0.563
4.887 ± 0.202
0.821 ± 0.173
3.162 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here