Botrytis cinerea mitovirus 2
Average proteome isoelectric point is 8.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4GA49|A0A0S4GA49_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 2 OX=1629665 GN=RdRp PE=4 SV=1
MM1 pKa = 6.92 KK2 pKa = 10.18 TKK4 pKa = 10.51 FLVTKK9 pKa = 10.34 RR10 pKa = 11.84 LLEE13 pKa = 4.06 SLFDD17 pKa = 4.36 DD18 pKa = 3.52 RR19 pKa = 11.84 TIGKK23 pKa = 9.75 KK24 pKa = 9.99 YY25 pKa = 9.17 IKK27 pKa = 10.22 SVEE30 pKa = 3.95 TMRR33 pKa = 11.84 IKK35 pKa = 10.68 SGLPFTIKK43 pKa = 10.49 YY44 pKa = 7.28 MKK46 pKa = 10.17 AVKK49 pKa = 10.12 LHH51 pKa = 4.48 ITRR54 pKa = 11.84 YY55 pKa = 9.89 ISGQPLRR62 pKa = 11.84 TNSSLVSLTNGFPTKK77 pKa = 10.49 FLYY80 pKa = 10.5 LKK82 pKa = 10.23 EE83 pKa = 5.23 LIDD86 pKa = 4.0 SGDD89 pKa = 4.0 PIKK92 pKa = 10.93 LRR94 pKa = 11.84 LVLTLTGYY102 pKa = 8.31 TRR104 pKa = 11.84 SIIPTKK110 pKa = 10.75 DD111 pKa = 2.89 EE112 pKa = 4.11 EE113 pKa = 4.57 KK114 pKa = 10.64 LVKK117 pKa = 9.05 PTFNSISDD125 pKa = 3.82 PYY127 pKa = 10.16 KK128 pKa = 10.22 GKK130 pKa = 10.63 EE131 pKa = 3.81 NYY133 pKa = 7.81 TIPTEE138 pKa = 4.53 FINGFVEE145 pKa = 4.61 KK146 pKa = 10.67 YY147 pKa = 10.17 KK148 pKa = 11.15 LNFMPKK154 pKa = 8.37 WDD156 pKa = 4.33 NSLQYY161 pKa = 10.31 ISNKK165 pKa = 9.71 SSPFGKK171 pKa = 8.51 STLTGPFALFHH182 pKa = 5.94 MGHH185 pKa = 6.97 WNLNMLDD192 pKa = 3.99 HH193 pKa = 6.77 FKK195 pKa = 11.33 SLIGDD200 pKa = 4.12 GSYY203 pKa = 10.93 KK204 pKa = 10.54 IMIGEE209 pKa = 4.02 LLEE212 pKa = 4.99 NVFKK216 pKa = 10.7 DD217 pKa = 3.43 HH218 pKa = 7.37 RR219 pKa = 11.84 CFHH222 pKa = 7.0 IGNSLNGIGKK232 pKa = 9.06 IAIVKK237 pKa = 10.29 DD238 pKa = 3.58 PEE240 pKa = 4.14 LKK242 pKa = 10.01 QRR244 pKa = 11.84 AIAMVDD250 pKa = 3.61 YY251 pKa = 10.72 YY252 pKa = 11.06 SQLVLRR258 pKa = 11.84 PIHH261 pKa = 7.41 DD262 pKa = 3.87 GLLNKK267 pKa = 9.71 LRR269 pKa = 11.84 NLPQDD274 pKa = 3.11 RR275 pKa = 11.84 TFTQNPFNNWGKK287 pKa = 8.89 TMGNSFWSLDD297 pKa = 3.57 LSSATDD303 pKa = 3.75 RR304 pKa = 11.84 FPISLQEE311 pKa = 4.09 KK312 pKa = 9.2 VLASILKK319 pKa = 10.35 DD320 pKa = 3.34 EE321 pKa = 5.22 AKK323 pKa = 10.82 AEE325 pKa = 4.17 TWRR328 pKa = 11.84 KK329 pKa = 9.25 ILIEE333 pKa = 4.31 RR334 pKa = 11.84 DD335 pKa = 3.38 YY336 pKa = 11.49 KK337 pKa = 11.09 LPNGNLTRR345 pKa = 11.84 YY346 pKa = 9.85 SVGQPMGAYY355 pKa = 10.07 SSWTAFTISHH365 pKa = 6.51 HH366 pKa = 6.03 LVVHH370 pKa = 6.49 FAAHH374 pKa = 6.48 LCGIEE379 pKa = 4.64 NFDD382 pKa = 4.69 KK383 pKa = 11.23 YY384 pKa = 11.07 ILLGDD389 pKa = 5.1 DD390 pKa = 3.46 IVINNDD396 pKa = 2.48 KK397 pKa = 10.69 VARR400 pKa = 11.84 KK401 pKa = 9.27 YY402 pKa = 10.71 IKK404 pKa = 10.28 IMTKK408 pKa = 10.23 LGVDD412 pKa = 3.21 ISINKK417 pKa = 7.45 THH419 pKa = 5.9 VSKK422 pKa = 9.84 NTYY425 pKa = 9.41 EE426 pKa = 4.06 FAKK429 pKa = 10.2 RR430 pKa = 11.84 WVKK433 pKa = 10.74 HH434 pKa = 5.65 NIEE437 pKa = 3.86 ITGIPLRR444 pKa = 11.84 GILNNLNSLPTAIKK458 pKa = 9.31 QLVYY462 pKa = 10.79 YY463 pKa = 9.58 FYY465 pKa = 11.34 SCNTLWRR472 pKa = 11.84 GNSVEE477 pKa = 4.75 LIFSIFKK484 pKa = 10.15 DD485 pKa = 2.82 IKK487 pKa = 10.11 LRR489 pKa = 11.84 NRR491 pKa = 11.84 FITDD495 pKa = 2.79 SKK497 pKa = 11.3 LRR499 pKa = 11.84 ILSEE503 pKa = 3.74 DD504 pKa = 3.64 TIFVIRR510 pKa = 11.84 QTLKK514 pKa = 10.12 LNTYY518 pKa = 8.86 EE519 pKa = 4.53 EE520 pKa = 3.86 IRR522 pKa = 11.84 NYY524 pKa = 10.08 FNKK527 pKa = 10.36 KK528 pKa = 8.2 LTIEE532 pKa = 4.08 NVLIPNEE539 pKa = 4.01 DD540 pKa = 3.62 QIHH543 pKa = 5.4 GFIRR547 pKa = 11.84 VILCLGLNKK556 pKa = 9.82 VAEE559 pKa = 4.25 KK560 pKa = 10.72 SGNDD564 pKa = 3.02 LSKK567 pKa = 10.75 YY568 pKa = 9.72 YY569 pKa = 10.82 KK570 pKa = 10.54 SFEE573 pKa = 4.18 KK574 pKa = 10.68 NFRR577 pKa = 11.84 KK578 pKa = 10.26 GDD580 pKa = 3.59 FDD582 pKa = 5.52 LKK584 pKa = 10.38 MLVRR588 pKa = 11.84 HH589 pKa = 6.45 PIVLGIYY596 pKa = 10.38 NKK598 pKa = 9.63 ISQMKK603 pKa = 8.8 RR604 pKa = 11.84 TLQKK608 pKa = 9.83 VRR610 pKa = 11.84 QMDD613 pKa = 3.9 EE614 pKa = 4.36 LDD616 pKa = 5.23 LIDD619 pKa = 5.5 AMTHH623 pKa = 4.67 MRR625 pKa = 11.84 IDD627 pKa = 3.78 EE628 pKa = 4.44 PDD630 pKa = 3.55 KK631 pKa = 11.07 LVQSLRR637 pKa = 11.84 NTSRR641 pKa = 11.84 SISHH645 pKa = 7.49 IDD647 pKa = 3.52 QLWKK651 pKa = 10.7 KK652 pKa = 9.32 GFKK655 pKa = 9.98 RR656 pKa = 11.84 LEE658 pKa = 4.35 IINEE662 pKa = 3.83 DD663 pKa = 3.4 NYY665 pKa = 10.88 VNFNEE670 pKa = 5.14 LDD672 pKa = 3.78 LPNKK676 pKa = 10.31 NNLKK680 pKa = 9.79 PWEE683 pKa = 4.22 SYY685 pKa = 10.64 YY686 pKa = 10.31 ISNLSEE692 pKa = 4.74 LSDD695 pKa = 3.97 KK696 pKa = 11.0 LDD698 pKa = 3.58 SLKK701 pKa = 10.14 TPVTQNMWFF710 pKa = 3.48
Molecular weight: 82.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 8.975
IPC_protein 8.843
Toseland 9.677
ProMoST 9.37
Dawson 9.911
Bjellqvist 9.575
Wikipedia 10.072
Rodwell 10.35
Grimsley 9.984
Solomon 9.926
Lehninger 9.882
Nozaki 9.692
DTASelect 9.56
Thurlkill 9.75
EMBOSS 10.101
Sillero 9.823
Patrickios 5.753
IPC_peptide 9.926
IPC2_peptide 8.009
IPC2.peptide.svr19 8.102
Protein with the highest isoelectric point:
>tr|A0A0S4GA49|A0A0S4GA49_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 2 OX=1629665 GN=RdRp PE=4 SV=1
MM1 pKa = 6.92 KK2 pKa = 10.18 TKK4 pKa = 10.51 FLVTKK9 pKa = 10.34 RR10 pKa = 11.84 LLEE13 pKa = 4.06 SLFDD17 pKa = 4.36 DD18 pKa = 3.52 RR19 pKa = 11.84 TIGKK23 pKa = 9.75 KK24 pKa = 9.99 YY25 pKa = 9.17 IKK27 pKa = 10.22 SVEE30 pKa = 3.95 TMRR33 pKa = 11.84 IKK35 pKa = 10.68 SGLPFTIKK43 pKa = 10.49 YY44 pKa = 7.28 MKK46 pKa = 10.17 AVKK49 pKa = 10.12 LHH51 pKa = 4.48 ITRR54 pKa = 11.84 YY55 pKa = 9.89 ISGQPLRR62 pKa = 11.84 TNSSLVSLTNGFPTKK77 pKa = 10.49 FLYY80 pKa = 10.5 LKK82 pKa = 10.23 EE83 pKa = 5.23 LIDD86 pKa = 4.0 SGDD89 pKa = 4.0 PIKK92 pKa = 10.93 LRR94 pKa = 11.84 LVLTLTGYY102 pKa = 8.31 TRR104 pKa = 11.84 SIIPTKK110 pKa = 10.75 DD111 pKa = 2.89 EE112 pKa = 4.11 EE113 pKa = 4.57 KK114 pKa = 10.64 LVKK117 pKa = 9.05 PTFNSISDD125 pKa = 3.82 PYY127 pKa = 10.16 KK128 pKa = 10.22 GKK130 pKa = 10.63 EE131 pKa = 3.81 NYY133 pKa = 7.81 TIPTEE138 pKa = 4.53 FINGFVEE145 pKa = 4.61 KK146 pKa = 10.67 YY147 pKa = 10.17 KK148 pKa = 11.15 LNFMPKK154 pKa = 8.37 WDD156 pKa = 4.33 NSLQYY161 pKa = 10.31 ISNKK165 pKa = 9.71 SSPFGKK171 pKa = 8.51 STLTGPFALFHH182 pKa = 5.94 MGHH185 pKa = 6.97 WNLNMLDD192 pKa = 3.99 HH193 pKa = 6.77 FKK195 pKa = 11.33 SLIGDD200 pKa = 4.12 GSYY203 pKa = 10.93 KK204 pKa = 10.54 IMIGEE209 pKa = 4.02 LLEE212 pKa = 4.99 NVFKK216 pKa = 10.7 DD217 pKa = 3.43 HH218 pKa = 7.37 RR219 pKa = 11.84 CFHH222 pKa = 7.0 IGNSLNGIGKK232 pKa = 9.06 IAIVKK237 pKa = 10.29 DD238 pKa = 3.58 PEE240 pKa = 4.14 LKK242 pKa = 10.01 QRR244 pKa = 11.84 AIAMVDD250 pKa = 3.61 YY251 pKa = 10.72 YY252 pKa = 11.06 SQLVLRR258 pKa = 11.84 PIHH261 pKa = 7.41 DD262 pKa = 3.87 GLLNKK267 pKa = 9.71 LRR269 pKa = 11.84 NLPQDD274 pKa = 3.11 RR275 pKa = 11.84 TFTQNPFNNWGKK287 pKa = 8.89 TMGNSFWSLDD297 pKa = 3.57 LSSATDD303 pKa = 3.75 RR304 pKa = 11.84 FPISLQEE311 pKa = 4.09 KK312 pKa = 9.2 VLASILKK319 pKa = 10.35 DD320 pKa = 3.34 EE321 pKa = 5.22 AKK323 pKa = 10.82 AEE325 pKa = 4.17 TWRR328 pKa = 11.84 KK329 pKa = 9.25 ILIEE333 pKa = 4.31 RR334 pKa = 11.84 DD335 pKa = 3.38 YY336 pKa = 11.49 KK337 pKa = 11.09 LPNGNLTRR345 pKa = 11.84 YY346 pKa = 9.85 SVGQPMGAYY355 pKa = 10.07 SSWTAFTISHH365 pKa = 6.51 HH366 pKa = 6.03 LVVHH370 pKa = 6.49 FAAHH374 pKa = 6.48 LCGIEE379 pKa = 4.64 NFDD382 pKa = 4.69 KK383 pKa = 11.23 YY384 pKa = 11.07 ILLGDD389 pKa = 5.1 DD390 pKa = 3.46 IVINNDD396 pKa = 2.48 KK397 pKa = 10.69 VARR400 pKa = 11.84 KK401 pKa = 9.27 YY402 pKa = 10.71 IKK404 pKa = 10.28 IMTKK408 pKa = 10.23 LGVDD412 pKa = 3.21 ISINKK417 pKa = 7.45 THH419 pKa = 5.9 VSKK422 pKa = 9.84 NTYY425 pKa = 9.41 EE426 pKa = 4.06 FAKK429 pKa = 10.2 RR430 pKa = 11.84 WVKK433 pKa = 10.74 HH434 pKa = 5.65 NIEE437 pKa = 3.86 ITGIPLRR444 pKa = 11.84 GILNNLNSLPTAIKK458 pKa = 9.31 QLVYY462 pKa = 10.79 YY463 pKa = 9.58 FYY465 pKa = 11.34 SCNTLWRR472 pKa = 11.84 GNSVEE477 pKa = 4.75 LIFSIFKK484 pKa = 10.15 DD485 pKa = 2.82 IKK487 pKa = 10.11 LRR489 pKa = 11.84 NRR491 pKa = 11.84 FITDD495 pKa = 2.79 SKK497 pKa = 11.3 LRR499 pKa = 11.84 ILSEE503 pKa = 3.74 DD504 pKa = 3.64 TIFVIRR510 pKa = 11.84 QTLKK514 pKa = 10.12 LNTYY518 pKa = 8.86 EE519 pKa = 4.53 EE520 pKa = 3.86 IRR522 pKa = 11.84 NYY524 pKa = 10.08 FNKK527 pKa = 10.36 KK528 pKa = 8.2 LTIEE532 pKa = 4.08 NVLIPNEE539 pKa = 4.01 DD540 pKa = 3.62 QIHH543 pKa = 5.4 GFIRR547 pKa = 11.84 VILCLGLNKK556 pKa = 9.82 VAEE559 pKa = 4.25 KK560 pKa = 10.72 SGNDD564 pKa = 3.02 LSKK567 pKa = 10.75 YY568 pKa = 9.72 YY569 pKa = 10.82 KK570 pKa = 10.54 SFEE573 pKa = 4.18 KK574 pKa = 10.68 NFRR577 pKa = 11.84 KK578 pKa = 10.26 GDD580 pKa = 3.59 FDD582 pKa = 5.52 LKK584 pKa = 10.38 MLVRR588 pKa = 11.84 HH589 pKa = 6.45 PIVLGIYY596 pKa = 10.38 NKK598 pKa = 9.63 ISQMKK603 pKa = 8.8 RR604 pKa = 11.84 TLQKK608 pKa = 9.83 VRR610 pKa = 11.84 QMDD613 pKa = 3.9 EE614 pKa = 4.36 LDD616 pKa = 5.23 LIDD619 pKa = 5.5 AMTHH623 pKa = 4.67 MRR625 pKa = 11.84 IDD627 pKa = 3.78 EE628 pKa = 4.44 PDD630 pKa = 3.55 KK631 pKa = 11.07 LVQSLRR637 pKa = 11.84 NTSRR641 pKa = 11.84 SISHH645 pKa = 7.49 IDD647 pKa = 3.52 QLWKK651 pKa = 10.7 KK652 pKa = 9.32 GFKK655 pKa = 9.98 RR656 pKa = 11.84 LEE658 pKa = 4.35 IINEE662 pKa = 3.83 DD663 pKa = 3.4 NYY665 pKa = 10.88 VNFNEE670 pKa = 5.14 LDD672 pKa = 3.78 LPNKK676 pKa = 10.31 NNLKK680 pKa = 9.79 PWEE683 pKa = 4.22 SYY685 pKa = 10.64 YY686 pKa = 10.31 ISNLSEE692 pKa = 4.74 LSDD695 pKa = 3.97 KK696 pKa = 11.0 LDD698 pKa = 3.58 SLKK701 pKa = 10.14 TPVTQNMWFF710 pKa = 3.48
Molecular weight: 82.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 8.975
IPC_protein 8.843
Toseland 9.677
ProMoST 9.37
Dawson 9.911
Bjellqvist 9.575
Wikipedia 10.072
Rodwell 10.35
Grimsley 9.984
Solomon 9.926
Lehninger 9.882
Nozaki 9.692
DTASelect 9.56
Thurlkill 9.75
EMBOSS 10.101
Sillero 9.823
Patrickios 5.753
IPC_peptide 9.926
IPC2_peptide 8.009
IPC2.peptide.svr19 8.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
710
710
710
710.0
82.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.535 ± 0.0
0.563 ± 0.0
5.634 ± 0.0
4.648 ± 0.0
5.07 ± 0.0
4.93 ± 0.0
2.394 ± 0.0
9.014 ± 0.0
9.859 ± 0.0
11.549 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.394 ± 0.0
7.324 ± 0.0
3.662 ± 0.0
2.394 ± 0.0
4.789 ± 0.0
7.183 ± 0.0
5.915 ± 0.0
4.507 ± 0.0
1.549 ± 0.0
4.085 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here