Amino acid dipepetide frequency for Ralstonia phage DU_RP_II

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.344AlaAla: 19.344 ± 2.171
1.162AlaCys: 1.162 ± 0.369
7.721AlaAsp: 7.721 ± 1.338
7.472AlaGlu: 7.472 ± 1.131
3.985AlaPhe: 3.985 ± 0.505
10.046AlaGly: 10.046 ± 1.016
2.408AlaHis: 2.408 ± 0.442
4.649AlaIle: 4.649 ± 0.578
5.895AlaLys: 5.895 ± 0.671
9.049AlaLeu: 9.049 ± 0.774
3.819AlaMet: 3.819 ± 0.776
7.14AlaAsn: 7.14 ± 1.8
6.061AlaPro: 6.061 ± 0.842
6.642AlaGln: 6.642 ± 0.806
6.559AlaArg: 6.559 ± 0.79
7.14AlaSer: 7.14 ± 0.695
7.14AlaThr: 7.14 ± 0.947
8.302AlaVal: 8.302 ± 0.804
1.494AlaTrp: 1.494 ± 0.377
4.566AlaTyr: 4.566 ± 0.524
0.0AlaXaa: 0.0 ± 0.0
Cys
0.913CysAla: 0.913 ± 0.296
0.083CysCys: 0.083 ± 0.094
0.498CysAsp: 0.498 ± 0.183
0.415CysGlu: 0.415 ± 0.178
0.498CysPhe: 0.498 ± 0.17
0.332CysGly: 0.332 ± 0.155
0.249CysHis: 0.249 ± 0.135
0.249CysIle: 0.249 ± 0.124
0.332CysLys: 0.332 ± 0.17
0.747CysLeu: 0.747 ± 0.229
0.083CysMet: 0.083 ± 0.071
0.498CysAsn: 0.498 ± 0.203
0.581CysPro: 0.581 ± 0.261
0.332CysGln: 0.332 ± 0.17
0.913CysArg: 0.913 ± 0.312
0.581CysSer: 0.581 ± 0.25
0.498CysThr: 0.498 ± 0.256
0.581CysVal: 0.581 ± 0.205
0.166CysTrp: 0.166 ± 0.123
0.249CysTyr: 0.249 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
8.385AspAla: 8.385 ± 1.732
0.415AspCys: 0.415 ± 0.193
2.823AspAsp: 2.823 ± 0.496
3.404AspGlu: 3.404 ± 0.634
1.743AspPhe: 1.743 ± 0.387
5.064AspGly: 5.064 ± 0.742
0.664AspHis: 0.664 ± 0.259
2.823AspIle: 2.823 ± 0.436
2.491AspLys: 2.491 ± 0.595
4.732AspLeu: 4.732 ± 1.001
1.494AspMet: 1.494 ± 0.351
1.91AspAsn: 1.91 ± 0.412
3.653AspPro: 3.653 ± 0.685
2.491AspGln: 2.491 ± 0.53
3.57AspArg: 3.57 ± 0.541
3.155AspSer: 3.155 ± 0.471
2.906AspThr: 2.906 ± 0.532
3.238AspVal: 3.238 ± 0.482
1.162AspTrp: 1.162 ± 0.375
1.743AspTyr: 1.743 ± 0.351
0.0AspXaa: 0.0 ± 0.0
Glu
7.057GluAla: 7.057 ± 1.044
0.581GluCys: 0.581 ± 0.165
1.494GluAsp: 1.494 ± 0.348
2.823GluGlu: 2.823 ± 0.524
1.162GluPhe: 1.162 ± 0.376
3.487GluGly: 3.487 ± 0.569
1.411GluHis: 1.411 ± 0.345
2.076GluIle: 2.076 ± 0.412
2.491GluLys: 2.491 ± 0.457
5.562GluLeu: 5.562 ± 0.875
1.91GluMet: 1.91 ± 0.406
1.411GluAsn: 1.411 ± 0.249
2.408GluPro: 2.408 ± 0.589
3.736GluGln: 3.736 ± 0.599
4.981GluArg: 4.981 ± 0.897
2.574GluSer: 2.574 ± 0.484
2.159GluThr: 2.159 ± 0.323
2.823GluVal: 2.823 ± 0.623
0.913GluTrp: 0.913 ± 0.279
1.328GluTyr: 1.328 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
3.072PheAla: 3.072 ± 0.496
0.332PheCys: 0.332 ± 0.147
1.91PheAsp: 1.91 ± 0.462
1.245PheGlu: 1.245 ± 0.279
1.245PhePhe: 1.245 ± 0.242
3.155PheGly: 3.155 ± 0.434
0.332PheHis: 0.332 ± 0.148
1.411PheIle: 1.411 ± 0.388
0.996PheLys: 0.996 ± 0.294
1.826PheLeu: 1.826 ± 0.276
0.747PheMet: 0.747 ± 0.253
1.494PheAsn: 1.494 ± 0.362
1.079PhePro: 1.079 ± 0.332
1.91PheGln: 1.91 ± 0.552
1.993PheArg: 1.993 ± 0.379
2.574PheSer: 2.574 ± 0.476
1.743PheThr: 1.743 ± 0.503
1.993PheVal: 1.993 ± 0.517
0.664PheTrp: 0.664 ± 0.217
0.913PheTyr: 0.913 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
11.706GlyAla: 11.706 ± 0.984
0.415GlyCys: 0.415 ± 0.172
3.653GlyAsp: 3.653 ± 0.783
4.068GlyGlu: 4.068 ± 0.431
2.491GlyPhe: 2.491 ± 0.537
9.215GlyGly: 9.215 ± 1.52
1.577GlyHis: 1.577 ± 0.354
3.819GlyIle: 3.819 ± 0.441
3.57GlyLys: 3.57 ± 0.535
6.559GlyLeu: 6.559 ± 0.889
2.159GlyMet: 2.159 ± 0.338
4.068GlyAsn: 4.068 ± 0.816
2.408GlyPro: 2.408 ± 0.427
4.068GlyGln: 4.068 ± 0.584
4.566GlyArg: 4.566 ± 0.689
5.479GlySer: 5.479 ± 0.951
5.895GlyThr: 5.895 ± 1.279
5.23GlyVal: 5.23 ± 0.851
1.494GlyTrp: 1.494 ± 0.269
2.74GlyTyr: 2.74 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
1.743HisAla: 1.743 ± 0.53
0.249HisCys: 0.249 ± 0.126
1.162HisAsp: 1.162 ± 0.287
0.83HisGlu: 0.83 ± 0.23
0.332HisPhe: 0.332 ± 0.202
1.577HisGly: 1.577 ± 0.39
0.415HisHis: 0.415 ± 0.211
0.996HisIle: 0.996 ± 0.317
0.747HisLys: 0.747 ± 0.247
1.577HisLeu: 1.577 ± 0.437
0.415HisMet: 0.415 ± 0.226
0.332HisAsn: 0.332 ± 0.178
0.996HisPro: 0.996 ± 0.271
0.498HisGln: 0.498 ± 0.171
1.743HisArg: 1.743 ± 0.471
0.83HisSer: 0.83 ± 0.271
0.747HisThr: 0.747 ± 0.22
0.747HisVal: 0.747 ± 0.196
0.415HisTrp: 0.415 ± 0.203
0.332HisTyr: 0.332 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
4.981IleAla: 4.981 ± 0.506
0.332IleCys: 0.332 ± 0.14
3.57IleAsp: 3.57 ± 0.566
2.408IleGlu: 2.408 ± 0.419
1.245IlePhe: 1.245 ± 0.378
4.483IleGly: 4.483 ± 0.676
0.747IleHis: 0.747 ± 0.221
1.577IleIle: 1.577 ± 0.443
2.491IleLys: 2.491 ± 0.443
2.657IleLeu: 2.657 ± 0.413
0.581IleMet: 0.581 ± 0.273
2.491IleAsn: 2.491 ± 0.328
2.159IlePro: 2.159 ± 0.513
0.913IleGln: 0.913 ± 0.223
1.91IleArg: 1.91 ± 0.405
2.325IleSer: 2.325 ± 0.772
3.819IleThr: 3.819 ± 0.833
2.325IleVal: 2.325 ± 0.531
0.664IleTrp: 0.664 ± 0.188
0.913IleTyr: 0.913 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
4.898LysAla: 4.898 ± 0.762
0.415LysCys: 0.415 ± 0.188
2.325LysAsp: 2.325 ± 0.517
1.826LysGlu: 1.826 ± 0.408
1.494LysPhe: 1.494 ± 0.333
2.408LysGly: 2.408 ± 0.504
0.664LysHis: 0.664 ± 0.218
1.826LysIle: 1.826 ± 0.276
1.411LysLys: 1.411 ± 0.314
4.151LysLeu: 4.151 ± 0.416
1.245LysMet: 1.245 ± 0.298
1.743LysAsn: 1.743 ± 0.425
1.993LysPro: 1.993 ± 0.344
3.072LysGln: 3.072 ± 0.636
3.321LysArg: 3.321 ± 0.644
2.159LysSer: 2.159 ± 0.455
2.242LysThr: 2.242 ± 0.424
2.408LysVal: 2.408 ± 0.377
1.245LysTrp: 1.245 ± 0.297
1.245LysTyr: 1.245 ± 0.315
0.0LysXaa: 0.0 ± 0.0
Leu
8.136LeuAla: 8.136 ± 0.737
0.581LeuCys: 0.581 ± 0.194
5.23LeuAsp: 5.23 ± 0.507
4.068LeuGlu: 4.068 ± 0.742
2.491LeuPhe: 2.491 ± 0.686
5.812LeuGly: 5.812 ± 0.686
1.577LeuHis: 1.577 ± 0.398
3.238LeuIle: 3.238 ± 0.595
3.321LeuLys: 3.321 ± 0.663
5.313LeuLeu: 5.313 ± 0.865
1.993LeuMet: 1.993 ± 0.355
2.076LeuAsn: 2.076 ± 0.384
4.566LeuPro: 4.566 ± 0.602
4.151LeuGln: 4.151 ± 0.697
6.891LeuArg: 6.891 ± 0.883
5.562LeuSer: 5.562 ± 1.648
5.645LeuThr: 5.645 ± 0.658
4.566LeuVal: 4.566 ± 0.703
1.162LeuTrp: 1.162 ± 0.308
1.494LeuTyr: 1.494 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
3.985MetAla: 3.985 ± 0.597
0.166MetCys: 0.166 ± 0.105
1.079MetAsp: 1.079 ± 0.221
1.66MetGlu: 1.66 ± 0.331
0.498MetPhe: 0.498 ± 0.168
2.74MetGly: 2.74 ± 0.455
0.166MetHis: 0.166 ± 0.116
0.581MetIle: 0.581 ± 0.192
1.162MetLys: 1.162 ± 0.314
1.993MetLeu: 1.993 ± 0.38
0.83MetMet: 0.83 ± 0.298
1.162MetAsn: 1.162 ± 0.343
0.996MetPro: 0.996 ± 0.259
1.079MetGln: 1.079 ± 0.393
1.91MetArg: 1.91 ± 0.474
1.245MetSer: 1.245 ± 0.3
2.242MetThr: 2.242 ± 0.356
1.162MetVal: 1.162 ± 0.429
0.415MetTrp: 0.415 ± 0.225
0.415MetTyr: 0.415 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
4.898AsnAla: 4.898 ± 0.698
0.415AsnCys: 0.415 ± 0.206
1.91AsnAsp: 1.91 ± 0.518
2.159AsnGlu: 2.159 ± 0.643
1.411AsnPhe: 1.411 ± 0.243
4.483AsnGly: 4.483 ± 0.707
0.664AsnHis: 0.664 ± 0.305
1.993AsnIle: 1.993 ± 0.575
1.577AsnLys: 1.577 ± 0.44
4.483AsnLeu: 4.483 ± 1.971
0.83AsnMet: 0.83 ± 0.262
1.66AsnAsn: 1.66 ± 0.53
1.743AsnPro: 1.743 ± 0.301
1.494AsnGln: 1.494 ± 0.419
2.076AsnArg: 2.076 ± 0.436
2.491AsnSer: 2.491 ± 0.571
2.408AsnThr: 2.408 ± 0.575
2.574AsnVal: 2.574 ± 0.626
0.747AsnTrp: 0.747 ± 0.173
1.079AsnTyr: 1.079 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
7.472ProAla: 7.472 ± 1.12
0.415ProCys: 0.415 ± 0.222
3.155ProAsp: 3.155 ± 0.689
3.072ProGlu: 3.072 ± 0.506
0.913ProPhe: 0.913 ± 0.362
4.4ProGly: 4.4 ± 0.62
0.498ProHis: 0.498 ± 0.295
1.411ProIle: 1.411 ± 0.316
1.328ProLys: 1.328 ± 0.282
2.989ProLeu: 2.989 ± 0.452
0.913ProMet: 0.913 ± 0.259
1.91ProAsn: 1.91 ± 0.367
2.574ProPro: 2.574 ± 0.483
2.325ProGln: 2.325 ± 0.659
2.491ProArg: 2.491 ± 0.568
2.491ProSer: 2.491 ± 0.53
3.321ProThr: 3.321 ± 0.525
2.989ProVal: 2.989 ± 0.474
0.664ProTrp: 0.664 ± 0.301
1.743ProTyr: 1.743 ± 0.36
0.0ProXaa: 0.0 ± 0.0
Gln
7.555GlnAla: 7.555 ± 0.788
0.166GlnCys: 0.166 ± 0.103
3.321GlnAsp: 3.321 ± 0.521
2.408GlnGlu: 2.408 ± 0.406
1.66GlnPhe: 1.66 ± 0.453
2.574GlnGly: 2.574 ± 0.647
0.747GlnHis: 0.747 ± 0.229
2.242GlnIle: 2.242 ± 0.511
2.076GlnLys: 2.076 ± 0.434
4.815GlnLeu: 4.815 ± 0.731
1.079GlnMet: 1.079 ± 0.293
1.079GlnAsn: 1.079 ± 0.334
1.577GlnPro: 1.577 ± 0.447
2.906GlnGln: 2.906 ± 0.66
4.234GlnArg: 4.234 ± 1.004
2.823GlnSer: 2.823 ± 0.574
2.491GlnThr: 2.491 ± 0.665
2.574GlnVal: 2.574 ± 0.51
1.079GlnTrp: 1.079 ± 0.349
1.577GlnTyr: 1.577 ± 0.376
0.0GlnXaa: 0.0 ± 0.0
Arg
7.721ArgAla: 7.721 ± 1.134
0.747ArgCys: 0.747 ± 0.206
3.238ArgAsp: 3.238 ± 0.631
3.155ArgGlu: 3.155 ± 0.653
2.906ArgPhe: 2.906 ± 0.632
4.981ArgGly: 4.981 ± 0.821
1.079ArgHis: 1.079 ± 0.287
3.902ArgIle: 3.902 ± 0.597
3.155ArgLys: 3.155 ± 0.694
3.321ArgLeu: 3.321 ± 0.398
1.245ArgMet: 1.245 ± 0.325
2.574ArgAsn: 2.574 ± 0.502
2.574ArgPro: 2.574 ± 0.321
2.408ArgGln: 2.408 ± 0.769
3.736ArgArg: 3.736 ± 0.66
2.906ArgSer: 2.906 ± 0.53
3.321ArgThr: 3.321 ± 0.641
5.895ArgVal: 5.895 ± 0.766
1.411ArgTrp: 1.411 ± 0.343
2.408ArgTyr: 2.408 ± 0.53
0.0ArgXaa: 0.0 ± 0.0
Ser
8.136SerAla: 8.136 ± 1.012
0.664SerCys: 0.664 ± 0.285
3.404SerAsp: 3.404 ± 0.794
1.743SerGlu: 1.743 ± 0.315
1.494SerPhe: 1.494 ± 0.362
6.476SerGly: 6.476 ± 1.253
0.664SerHis: 0.664 ± 0.225
3.155SerIle: 3.155 ± 0.677
2.408SerLys: 2.408 ± 0.463
3.487SerLeu: 3.487 ± 0.576
1.91SerMet: 1.91 ± 0.338
2.325SerAsn: 2.325 ± 0.566
2.989SerPro: 2.989 ± 0.726
2.823SerGln: 2.823 ± 0.765
2.242SerArg: 2.242 ± 0.514
3.238SerSer: 3.238 ± 0.992
3.902SerThr: 3.902 ± 0.894
3.985SerVal: 3.985 ± 0.915
0.498SerTrp: 0.498 ± 0.238
2.159SerTyr: 2.159 ± 0.68
0.0SerXaa: 0.0 ± 0.0
Thr
7.472ThrAla: 7.472 ± 1.034
0.249ThrCys: 0.249 ± 0.121
4.317ThrAsp: 4.317 ± 0.647
3.072ThrGlu: 3.072 ± 0.521
1.826ThrPhe: 1.826 ± 0.434
5.562ThrGly: 5.562 ± 1.115
0.996ThrHis: 0.996 ± 0.212
2.989ThrIle: 2.989 ± 0.872
2.159ThrLys: 2.159 ± 0.454
5.396ThrLeu: 5.396 ± 0.661
1.411ThrMet: 1.411 ± 0.335
1.91ThrAsn: 1.91 ± 0.578
4.649ThrPro: 4.649 ± 0.647
3.404ThrGln: 3.404 ± 0.648
2.989ThrArg: 2.989 ± 0.642
2.657ThrSer: 2.657 ± 0.56
5.064ThrThr: 5.064 ± 1.14
5.23ThrVal: 5.23 ± 1.064
0.664ThrTrp: 0.664 ± 0.276
1.328ThrTyr: 1.328 ± 0.373
0.0ThrXaa: 0.0 ± 0.0
Val
7.887ValAla: 7.887 ± 0.779
0.83ValCys: 0.83 ± 0.306
4.649ValAsp: 4.649 ± 0.777
4.649ValGlu: 4.649 ± 0.754
2.159ValPhe: 2.159 ± 0.475
3.985ValGly: 3.985 ± 0.585
1.162ValHis: 1.162 ± 0.367
2.325ValIle: 2.325 ± 0.526
2.408ValLys: 2.408 ± 0.49
4.566ValLeu: 4.566 ± 0.708
1.66ValMet: 1.66 ± 0.323
3.404ValAsn: 3.404 ± 0.746
2.906ValPro: 2.906 ± 0.478
2.906ValGln: 2.906 ± 0.63
3.155ValArg: 3.155 ± 0.543
4.234ValSer: 4.234 ± 0.999
4.981ValThr: 4.981 ± 0.854
3.819ValVal: 3.819 ± 0.51
0.747ValTrp: 0.747 ± 0.374
0.996ValTyr: 0.996 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
1.993TrpAla: 1.993 ± 0.357
0.249TrpCys: 0.249 ± 0.136
0.581TrpAsp: 0.581 ± 0.167
0.913TrpGlu: 0.913 ± 0.274
0.747TrpPhe: 0.747 ± 0.336
1.079TrpGly: 1.079 ± 0.291
0.166TrpHis: 0.166 ± 0.121
0.332TrpIle: 0.332 ± 0.14
0.996TrpLys: 0.996 ± 0.248
2.242TrpLeu: 2.242 ± 0.449
0.581TrpMet: 0.581 ± 0.22
0.498TrpAsn: 0.498 ± 0.274
0.166TrpPro: 0.166 ± 0.101
0.996TrpGln: 0.996 ± 0.294
1.079TrpArg: 1.079 ± 0.281
1.079TrpSer: 1.079 ± 0.298
0.996TrpThr: 0.996 ± 0.245
1.245TrpVal: 1.245 ± 0.258
0.664TrpTrp: 0.664 ± 0.262
0.415TrpTyr: 0.415 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.238TyrAla: 3.238 ± 0.917
0.332TyrCys: 0.332 ± 0.164
1.826TyrAsp: 1.826 ± 0.288
0.996TyrGlu: 0.996 ± 0.253
0.332TyrPhe: 0.332 ± 0.208
3.321TyrGly: 3.321 ± 0.501
0.498TyrHis: 0.498 ± 0.199
0.996TyrIle: 0.996 ± 0.296
1.079TyrLys: 1.079 ± 0.304
2.408TyrLeu: 2.408 ± 0.507
0.498TyrMet: 0.498 ± 0.15
1.411TyrAsn: 1.411 ± 0.33
0.996TyrPro: 0.996 ± 0.282
0.83TyrGln: 0.83 ± 0.267
2.325TyrArg: 2.325 ± 0.527
2.242TyrSer: 2.242 ± 0.35
1.826TyrThr: 1.826 ± 0.442
1.743TyrVal: 1.743 ± 0.469
0.747TyrTrp: 0.747 ± 0.173
0.83TyrTyr: 0.83 ± 0.303
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (12046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski