Sphingomonas deserti
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5377 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P7QVY5|A0A2P7QVY5_9SPHN Uncharacterized protein OS=Sphingomonas deserti OX=2116704 GN=C7I55_07815 PE=4 SV=1
MM1 pKa = 7.38 ATRR4 pKa = 11.84 AYY6 pKa = 9.34 TADD9 pKa = 3.47 HH10 pKa = 6.89 LNNQLTLPPTLAFMLGVPHH29 pKa = 7.52 APRR32 pKa = 11.84 KK33 pKa = 8.68 IVEE36 pKa = 4.15 RR37 pKa = 11.84 VIQRR41 pKa = 11.84 AIDD44 pKa = 3.62 EE45 pKa = 5.11 LDD47 pKa = 3.73 TLDD50 pKa = 4.74 GDD52 pKa = 4.45 PDD54 pKa = 3.69 LEE56 pKa = 4.31 EE57 pKa = 4.73 PVLEE61 pKa = 4.56 DD62 pKa = 4.11 SFVKK66 pKa = 10.33 HH67 pKa = 6.27 DD68 pKa = 4.22 PYY70 pKa = 8.81 WTRR73 pKa = 11.84 GAGCEE78 pKa = 4.31 VSDD81 pKa = 5.29 PDD83 pKa = 4.1 CCEE86 pKa = 4.28 AGDD89 pKa = 5.3 DD90 pKa = 4.58 VGCQYY95 pKa = 11.0 QVYY98 pKa = 8.67 SHH100 pKa = 6.35 YY101 pKa = 11.32 AVGVDD106 pKa = 5.14 DD107 pKa = 6.16 GPGDD111 pKa = 4.07 PDD113 pKa = 4.73 DD114 pKa = 6.32 AEE116 pKa = 6.46 DD117 pKa = 5.37 DD118 pKa = 4.28 DD119 pKa = 5.1 PSGGNVDD126 pKa = 3.77 DD127 pKa = 3.68 QGEE130 pKa = 4.2 RR131 pKa = 11.84 EE132 pKa = 4.03 EE133 pKa = 4.87 AFF135 pKa = 3.52
Molecular weight: 14.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.745
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A2P7QP60|A0A2P7QP60_9SPHN Uncharacterized protein OS=Sphingomonas deserti OX=2116704 GN=C7I55_14250 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.51 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.81 ATVGGRR28 pKa = 11.84 KK29 pKa = 9.05 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5377
0
5377
1777423
26
6182
330.6
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.57 ± 0.053
0.733 ± 0.011
5.933 ± 0.041
5.462 ± 0.036
3.571 ± 0.022
9.216 ± 0.076
1.866 ± 0.017
4.843 ± 0.021
2.497 ± 0.023
9.954 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.069 ± 0.018
2.523 ± 0.029
5.39 ± 0.041
3.03 ± 0.021
7.896 ± 0.045
5.545 ± 0.033
5.27 ± 0.041
7.053 ± 0.024
1.408 ± 0.015
2.17 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here