Vibrio phage LP.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R5U5|A0A3Q9R5U5_9CAUD Uncharacterized protein OS=Vibrio phage LP.2 OX=2500151 PE=4 SV=1
MM1 pKa = 7.27TKK3 pKa = 10.36AILSLDD9 pKa = 3.69VYY11 pKa = 9.24VYY13 pKa = 10.81CPNCEE18 pKa = 3.83NSIDD22 pKa = 4.4LLDD25 pKa = 5.07GYY27 pKa = 10.92DD28 pKa = 4.59DD29 pKa = 4.11EE30 pKa = 6.58GEE32 pKa = 4.5VMNQACPTDD41 pKa = 3.65GTYY44 pKa = 9.81WGAAHH49 pKa = 7.6RR50 pKa = 11.84DD51 pKa = 3.72FEE53 pKa = 4.73IEE55 pKa = 5.1GVYY58 pKa = 10.53CDD60 pKa = 3.79NCDD63 pKa = 3.91HH64 pKa = 6.81EE65 pKa = 4.92FTVKK69 pKa = 10.63GIEE72 pKa = 4.17YY73 pKa = 10.57

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R5V2|A0A3Q9R5V2_9CAUD Coil containing protein OS=Vibrio phage LP.2 OX=2500151 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84WGTRR6 pKa = 11.84GDD8 pKa = 3.46NFMSIYY14 pKa = 9.96KK15 pKa = 9.93KK16 pKa = 10.18KK17 pKa = 10.7RR18 pKa = 11.84PFKK21 pKa = 10.52VIRR24 pKa = 11.84DD25 pKa = 3.48KK26 pKa = 11.59YY27 pKa = 10.53RR28 pKa = 11.84FFMCIGRR35 pKa = 11.84LRR37 pKa = 11.84VNIYY41 pKa = 9.66NPSISRR47 pKa = 11.84VEE49 pKa = 3.96PPKK52 pKa = 10.9DD53 pKa = 3.18QQ54 pKa = 3.81

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

11401

48

926

167.7

18.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.657 ± 0.639

1.324 ± 0.149

6.289 ± 0.2

7.113 ± 0.337

3.631 ± 0.173

6.622 ± 0.284

1.561 ± 0.204

6.219 ± 0.223

6.473 ± 0.519

7.868 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.216

5.438 ± 0.273

3.166 ± 0.188

4.684 ± 0.324

4.763 ± 0.244

6.903 ± 0.27

6.245 ± 0.475

6.245 ± 0.302

1.272 ± 0.116

3.798 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski