Arthrobacter phage DrYang
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9J7J1|A0A6B9J7J1_9CAUD Uncharacterized protein OS=Arthrobacter phage DrYang OX=2686080 GN=52 PE=4 SV=1
MM1 pKa = 6.71 TTTFEE6 pKa = 3.86 EE7 pKa = 3.96 ARR9 pKa = 11.84 AIVLEE14 pKa = 3.99 QNRR17 pKa = 11.84 EE18 pKa = 3.65 GWEE21 pKa = 3.94 EE22 pKa = 3.78 DD23 pKa = 3.29 HH24 pKa = 7.19 EE25 pKa = 5.03 GTFTVAAYY33 pKa = 9.77 GWEE36 pKa = 4.12 DD37 pKa = 3.41 DD38 pKa = 4.04 AGFLVVQGAHH48 pKa = 5.3 EE49 pKa = 4.15 WLVDD53 pKa = 3.37 GDD55 pKa = 3.89 EE56 pKa = 4.86 TYY58 pKa = 11.62 SVMDD62 pKa = 4.26 APAVFVSKK70 pKa = 10.95 DD71 pKa = 3.15 DD72 pKa = 3.95 GTILEE77 pKa = 4.46 ADD79 pKa = 3.98 YY80 pKa = 11.43 LDD82 pKa = 3.79 VADD85 pKa = 6.3 RR86 pKa = 11.84 IAAMTPVPGHH96 pKa = 6.77 EE97 pKa = 4.23 YY98 pKa = 10.68 TEE100 pKa = 4.52 DD101 pKa = 3.17
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.98
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.516
ProMoST 3.821
Dawson 3.694
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.427
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.834
Patrickios 2.804
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A6B9JC39|A0A6B9JC39_9CAUD Protease OS=Arthrobacter phage DrYang OX=2686080 GN=104 PE=4 SV=1
MM1 pKa = 7.24 TSYY4 pKa = 11.29 RR5 pKa = 11.84 DD6 pKa = 3.19 TSTRR10 pKa = 11.84 KK11 pKa = 9.15 HH12 pKa = 5.04 ATTGRR17 pKa = 11.84 RR18 pKa = 11.84 AADD21 pKa = 3.55 YY22 pKa = 9.55 TPAPLEE28 pKa = 3.96 PRR30 pKa = 11.84 KK31 pKa = 10.42 GRR33 pKa = 11.84 GPGKK37 pKa = 9.4 PKK39 pKa = 9.71 PVEE42 pKa = 3.89 RR43 pKa = 11.84 KK44 pKa = 8.73 EE45 pKa = 3.39 QDD47 pKa = 2.89 RR48 pKa = 11.84 RR49 pKa = 11.84 HH50 pKa = 5.76 DD51 pKa = 3.52 HH52 pKa = 6.28
Molecular weight: 6.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.736
IPC_protein 10.584
Toseland 10.833
ProMoST 10.54
Dawson 10.906
Bjellqvist 10.613
Wikipedia 11.125
Rodwell 11.125
Grimsley 10.95
Solomon 11.052
Lehninger 11.023
Nozaki 10.804
DTASelect 10.613
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.847
Patrickios 10.906
IPC_peptide 11.067
IPC2_peptide 9.385
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
105
0
105
21996
32
1256
209.5
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.366 ± 0.494
0.796 ± 0.109
5.815 ± 0.159
6.165 ± 0.368
2.855 ± 0.156
8.256 ± 0.269
2.26 ± 0.186
3.86 ± 0.21
4.314 ± 0.234
7.642 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.319 ± 0.099
3.819 ± 0.155
5.947 ± 0.24
3.382 ± 0.14
6.033 ± 0.262
4.965 ± 0.271
7.488 ± 0.305
7.46 ± 0.236
1.809 ± 0.141
2.45 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here