Flavobacterium phage fF4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SPW4|A0A6B9SPW4_9CAUD Putative repressor OS=Flavobacterium phage fF4 OX=2692741 PE=4 SV=1
MM1 pKa = 7.33NKK3 pKa = 9.96DD4 pKa = 3.42FEE6 pKa = 5.31LDD8 pKa = 3.24QDD10 pKa = 3.83GDD12 pKa = 4.21LLFVNGDD19 pKa = 3.45ISPAPSDD26 pKa = 3.59QQHH29 pKa = 6.36IEE31 pKa = 4.41HH32 pKa = 6.86ILEE35 pKa = 4.3AVKK38 pKa = 10.83GEE40 pKa = 4.03YY41 pKa = 9.43KK42 pKa = 10.39QYY44 pKa = 8.18PTVGFGIVNYY54 pKa = 10.47LKK56 pKa = 10.18TNVSEE61 pKa = 5.1AEE63 pKa = 4.16FKK65 pKa = 10.63RR66 pKa = 11.84DD67 pKa = 3.79LKK69 pKa = 10.93IQLEE73 pKa = 3.91QDD75 pKa = 2.87NYY77 pKa = 9.86KK78 pKa = 10.33NCRR81 pKa = 11.84IDD83 pKa = 3.52LTNGQLKK90 pKa = 10.07IDD92 pKa = 3.79VV93 pKa = 3.98

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SRP7|A0A6B9SRP7_9CAUD Uncharacterized protein OS=Flavobacterium phage fF4 OX=2692741 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 5.98LDD4 pKa = 4.05FRR6 pKa = 11.84DD7 pKa = 3.9EE8 pKa = 4.25YY9 pKa = 11.23FEE11 pKa = 4.05KK12 pKa = 10.59LSRR15 pKa = 11.84ISRR18 pKa = 11.84HH19 pKa = 5.14GFLKK23 pKa = 10.64RR24 pKa = 11.84LTDD27 pKa = 3.65HH28 pKa = 7.12AGVTAVNFVKK38 pKa = 10.51QRR40 pKa = 11.84FVEE43 pKa = 4.66KK44 pKa = 10.11NWKK47 pKa = 10.25DD48 pKa = 3.29NGTQPWEE55 pKa = 3.53ARR57 pKa = 11.84KK58 pKa = 9.98RR59 pKa = 11.84KK60 pKa = 9.65GRR62 pKa = 11.84GSLLTVSGRR71 pKa = 11.84LKK73 pKa = 10.57RR74 pKa = 11.84SIRR77 pKa = 11.84KK78 pKa = 8.6IASGNYY84 pKa = 8.17FVYY87 pKa = 10.3IGTDD91 pKa = 2.98VPYY94 pKa = 10.79AQVHH98 pKa = 5.38NEE100 pKa = 3.62GGEE103 pKa = 4.07TSKK106 pKa = 9.91TVNVRR111 pKa = 11.84SHH113 pKa = 3.65QRR115 pKa = 11.84KK116 pKa = 9.35RR117 pKa = 11.84NGRR120 pKa = 11.84SHH122 pKa = 6.18NVKK125 pKa = 10.04LHH127 pKa = 5.23TRR129 pKa = 11.84TSRR132 pKa = 11.84FKK134 pKa = 9.75VPKK137 pKa = 9.86RR138 pKa = 11.84QFLGDD143 pKa = 3.61SQYY146 pKa = 11.16LARR149 pKa = 11.84GIEE152 pKa = 4.14LMLKK156 pKa = 9.68QAIDD160 pKa = 3.52NEE162 pKa = 4.68LNQSS166 pKa = 3.35

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10072

47

746

201.4

22.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.027 ± 0.382

0.993 ± 0.131

5.451 ± 0.23

6.831 ± 0.257

4.716 ± 0.26

5.5 ± 0.267

1.559 ± 0.124

7.317 ± 0.408

9.601 ± 0.344

9.323 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.138

6.444 ± 0.283

2.81 ± 0.158

4.18 ± 0.216

3.505 ± 0.225

6.324 ± 0.294

5.818 ± 0.245

6.116 ± 0.294

1.221 ± 0.115

3.803 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski