Microplitis demolitor bracovirus (isolate Webb) (MdBV)

Taxonomy: Viruses; Polydnaviridae; Bracovirus; Microplitis demolitor bracovirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5I159|IKBC2_MDBVW I-Kappa-B like protein C2 OS=Microplitis demolitor bracovirus (isolate Webb) OX=654919 GN=C2 PE=3 SV=1
MM1 pKa = 7.21VLNLIIPQEE10 pKa = 4.15WNILPCATLQVEE22 pKa = 4.61LLRR25 pKa = 11.84IIRR28 pKa = 11.84AKK30 pKa = 10.35KK31 pKa = 10.45KK32 pKa = 10.55SDD34 pKa = 3.28LTSAGVVGYY43 pKa = 10.54YY44 pKa = 10.29NDD46 pKa = 3.47VVAEE50 pKa = 4.11SMDD53 pKa = 4.04FFDD56 pKa = 5.53CLWFRR61 pKa = 11.84KK62 pKa = 10.28LEE64 pKa = 4.33DD65 pKa = 3.36TSKK68 pKa = 11.06EE69 pKa = 4.1PDD71 pKa = 3.15FHH73 pKa = 7.57RR74 pKa = 11.84YY75 pKa = 9.4SLMIVPMVPSSAADD89 pKa = 3.4SDD91 pKa = 4.6DD92 pKa = 4.02SSSCSEE98 pKa = 4.34CDD100 pKa = 3.39SDD102 pKa = 6.21ALLSDD107 pKa = 5.49DD108 pKa = 5.35GPCSTCDD115 pKa = 3.11EE116 pKa = 4.55CHH118 pKa = 7.45DD119 pKa = 3.77SDD121 pKa = 5.15RR122 pKa = 11.84YY123 pKa = 10.19EE124 pKa = 4.55LSSDD128 pKa = 3.71CC129 pKa = 5.52

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5I131|YM5_MDBVW Uncharacterized protein M5 OS=Microplitis demolitor bracovirus (isolate Webb) OX=654919 GN=M5 PE=4 SV=1
MM1 pKa = 7.23FQPIYY6 pKa = 10.28RR7 pKa = 11.84SNLVIVITLFVSLSYY22 pKa = 11.18YY23 pKa = 7.69HH24 pKa = 6.24TCFVRR29 pKa = 11.84KK30 pKa = 8.6RR31 pKa = 11.84AHH33 pKa = 6.77IIVQILLCQLFILSCNRR50 pKa = 11.84SITLLTAWRR59 pKa = 11.84YY60 pKa = 7.47LHH62 pKa = 6.75VMSLILQRR70 pKa = 11.84CGIIVLKK77 pKa = 10.55IKK79 pKa = 10.68LLFGSDD85 pKa = 3.25VKK87 pKa = 11.03IFMLLNYY94 pKa = 10.04GVIKK98 pKa = 10.4FFSKK102 pKa = 10.9

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10188

68

515

192.2

22.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.761 ± 0.252

2.601 ± 0.196

5.163 ± 0.206

7.038 ± 0.402

3.985 ± 0.29

4.132 ± 0.204

2.631 ± 0.2

7.362 ± 0.222

7.067 ± 0.295

8.068 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.205

7.038 ± 0.305

3.426 ± 0.208

3.779 ± 0.198

4.967 ± 0.247

8.176 ± 0.478

6.606 ± 0.447

5.399 ± 0.318

1.492 ± 0.135

3.7 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski