Tetrahymena thermophila (strain SB210)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae; Tetrahymena; Tetrahymena thermophila

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7X298|W7X298_TETTS Transmembrane protein putative OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_000469239 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.58NCDD5 pKa = 3.58NQIGAIGASGLGSALANCINLKK27 pKa = 10.32NLTVDD32 pKa = 4.61LSWNQIGDD40 pKa = 3.66EE41 pKa = 4.27GASGLGSALANCINLSNLTLDD62 pKa = 3.45LHH64 pKa = 6.78KK65 pKa = 11.14NQIADD70 pKa = 3.74EE71 pKa = 4.53GASGLGSALANCINLSNLTLKK92 pKa = 10.94LVDD95 pKa = 3.73NQIGDD100 pKa = 3.78EE101 pKa = 4.26GASGLGSALANCINLSNLTLDD122 pKa = 4.21LGEE125 pKa = 4.19NQIGDD130 pKa = 3.83EE131 pKa = 4.34GASGLGSALANCINLSNLTLDD152 pKa = 4.07LQQKK156 pKa = 8.47QFII159 pKa = 4.18

Molecular weight: 16.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7XE92|W7XE92_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_000903859 PE=4 SV=1
TT1 pKa = 6.98QMGRR5 pKa = 11.84KK6 pKa = 8.65QLLPKK11 pKa = 9.78QLNVLISPLQASTLGLIIQIQMGRR35 pKa = 11.84KK36 pKa = 8.62QLLPKK41 pKa = 9.07QQNVLISPLQASTLSLINLIQMGRR65 pKa = 11.84KK66 pKa = 8.65QLLPKK71 pKa = 9.78QLNVLISPLQSLTLGGIKK89 pKa = 9.36QVQMGRR95 pKa = 11.84KK96 pKa = 8.62QLLPKK101 pKa = 9.78QLNVLISPFQAQTFRR116 pKa = 11.84LIIQIQMGRR125 pKa = 11.84KK126 pKa = 8.73HH127 pKa = 6.7LLPKK131 pKa = 10.14QLNLLISPLQASTFF145 pKa = 3.65

Molecular weight: 16.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26971

5

26976

16915139

27

8271

627.0

72.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.264 ± 0.01

1.787 ± 0.021

4.844 ± 0.01

5.96 ± 0.021

5.179 ± 0.012

3.467 ± 0.013

1.442 ± 0.006

8.632 ± 0.018

8.756 ± 0.02

9.189 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.788 ± 0.005

9.18 ± 0.019

2.592 ± 0.009

9.62 ± 0.025

2.71 ± 0.009

8.315 ± 0.015

4.399 ± 0.013

3.985 ± 0.009

0.507 ± 0.003

4.314 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski