Amino acid dipepetide frequency for Clostridium leptum CAG:27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.502AlaAla: 9.502 ± 0.173	1.429AlaCys: 1.429 ± 0.046	4.334AlaAsp: 4.334 ± 0.093	5.57AlaGlu: 5.57 ± 0.086	3.521AlaPhe: 3.521 ± 0.078	6.961AlaGly: 6.961 ± 0.098	1.175AlaHis: 1.175 ± 0.049	4.616AlaIle: 4.616 ± 0.095	4.665AlaLys: 4.665 ± 0.081	8.334AlaLeu: 8.334 ± 0.106	2.619AlaMet: 2.619 ± 0.061	2.408AlaAsn: 2.408 ± 0.065	2.749AlaPro: 2.749 ± 0.068	3.256AlaGln: 3.256 ± 0.078	3.624AlaArg: 3.624 ± 0.069	4.845AlaSer: 4.845 ± 0.1	3.054AlaThr: 3.054 ± 0.071	7.726AlaVal: 7.726 ± 0.118	0.809AlaTrp: 0.809 ± 0.029	2.815AlaTyr: 2.815 ± 0.063	0.001AlaXaa: 0.001 ± 0.001
Cys	1.285CysAla: 1.285 ± 0.043	0.325CysCys: 0.325 ± 0.02	0.907CysAsp: 0.907 ± 0.037	0.953CysGlu: 0.953 ± 0.038	0.751CysPhe: 0.751 ± 0.03	1.882CysGly: 1.882 ± 0.058	0.32CysHis: 0.32 ± 0.022	0.883CysIle: 0.883 ± 0.039	0.8CysLys: 0.8 ± 0.033	1.333CysLeu: 1.333 ± 0.045	0.401CysMet: 0.401 ± 0.025	0.52CysAsn: 0.52 ± 0.028	0.782CysPro: 0.782 ± 0.034	0.527CysGln: 0.527 ± 0.029	0.986CysArg: 0.986 ± 0.04	0.985CysSer: 0.985 ± 0.04	0.733CysThr: 0.733 ± 0.032	1.174CysVal: 1.174 ± 0.044	0.142CysTrp: 0.142 ± 0.014	0.588CysTyr: 0.588 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.024AspAla: 4.024 ± 0.077	0.862AspCys: 0.862 ± 0.033	2.516AspAsp: 2.516 ± 0.064	3.7AspGlu: 3.7 ± 0.069	2.683AspPhe: 2.683 ± 0.053	4.487AspGly: 4.487 ± 0.111	0.924AspHis: 0.924 ± 0.041	3.568AspIle: 3.568 ± 0.078	2.626AspLys: 2.626 ± 0.069	4.675AspLeu: 4.675 ± 0.082	1.429AspMet: 1.429 ± 0.045	1.848AspAsn: 1.848 ± 0.059	2.386AspPro: 2.386 ± 0.064	1.899AspGln: 1.899 ± 0.054	2.491AspArg: 2.491 ± 0.055	3.122AspSer: 3.122 ± 0.069	2.814AspThr: 2.814 ± 0.072	3.401AspVal: 3.401 ± 0.062	0.736AspTrp: 0.736 ± 0.033	2.45AspTyr: 2.45 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	6.402GluAla: 6.402 ± 0.094	0.769GluCys: 0.769 ± 0.032	3.598GluAsp: 3.598 ± 0.069	6.101GluGlu: 6.101 ± 0.099	2.555GluPhe: 2.555 ± 0.067	4.377GluGly: 4.377 ± 0.07	1.122GluHis: 1.122 ± 0.035	5.147GluIle: 5.147 ± 0.096	5.678GluLys: 5.678 ± 0.083	6.85GluLeu: 6.85 ± 0.114	2.151GluMet: 2.151 ± 0.056	3.712GluAsn: 3.712 ± 0.068	2.196GluPro: 2.196 ± 0.064	2.661GluGln: 2.661 ± 0.067	3.562GluArg: 3.562 ± 0.084	3.418GluSer: 3.418 ± 0.069	3.543GluThr: 3.543 ± 0.058	3.732GluVal: 3.732 ± 0.078	0.656GluTrp: 0.656 ± 0.034	2.587GluTyr: 2.587 ± 0.064	0.001GluXaa: 0.001 ± 0.001
Phe	3.166PheAla: 3.166 ± 0.078	0.892PheCys: 0.892 ± 0.034	2.508PheAsp: 2.508 ± 0.063	2.537PheGlu: 2.537 ± 0.066	2.033PhePhe: 2.033 ± 0.065	3.211PheGly: 3.211 ± 0.07	0.91PheHis: 0.91 ± 0.033	2.487PheIle: 2.487 ± 0.065	1.597PheLys: 1.597 ± 0.051	4.288PheLeu: 4.288 ± 0.094	0.998PheMet: 0.998 ± 0.034	1.453PheAsn: 1.453 ± 0.049	1.629PhePro: 1.629 ± 0.041	1.865PheGln: 1.865 ± 0.043	1.975PheArg: 1.975 ± 0.05	3.418PheSer: 3.418 ± 0.075	2.42PheThr: 2.42 ± 0.06	2.757PheVal: 2.757 ± 0.059	0.489PheTrp: 0.489 ± 0.026	1.782PheTyr: 1.782 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	6.303GlyAla: 6.303 ± 0.111	1.552GlyCys: 1.552 ± 0.056	3.651GlyAsp: 3.651 ± 0.084	5.099GlyGlu: 5.099 ± 0.083	3.304GlyPhe: 3.304 ± 0.075	5.883GlyGly: 5.883 ± 0.117	1.224GlyHis: 1.224 ± 0.043	5.613GlyIle: 5.613 ± 0.096	5.062GlyLys: 5.062 ± 0.09	6.82GlyLeu: 6.82 ± 0.111	2.384GlyMet: 2.384 ± 0.056	2.741GlyAsn: 2.741 ± 0.069	1.738GlyPro: 1.738 ± 0.058	2.412GlyGln: 2.412 ± 0.059	3.756GlyArg: 3.756 ± 0.088	4.377GlySer: 4.377 ± 0.076	4.104GlyThr: 4.104 ± 0.09	5.828GlyVal: 5.828 ± 0.094	0.815GlyTrp: 0.815 ± 0.045	3.142GlyTyr: 3.142 ± 0.068	0.004GlyXaa: 0.004 ± 0.003
His	1.101HisAla: 1.101 ± 0.039	0.347HisCys: 0.347 ± 0.022	0.766HisAsp: 0.766 ± 0.032	0.89HisGlu: 0.89 ± 0.036	0.855HisPhe: 0.855 ± 0.036	1.402HisGly: 1.402 ± 0.049	0.38HisHis: 0.38 ± 0.024	1.158HisIle: 1.158 ± 0.04	0.824HisLys: 0.824 ± 0.036	1.495HisLeu: 1.495 ± 0.047	0.479HisMet: 0.479 ± 0.026	0.695HisAsn: 0.695 ± 0.028	0.989HisPro: 0.989 ± 0.035	0.637HisGln: 0.637 ± 0.027	0.893HisArg: 0.893 ± 0.042	0.965HisSer: 0.965 ± 0.043	0.877HisThr: 0.877 ± 0.036	1.055HisVal: 1.055 ± 0.033	0.238HisTrp: 0.238 ± 0.019	0.723HisTyr: 0.723 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.297IleAla: 5.297 ± 0.094	1.148IleCys: 1.148 ± 0.038	3.761IleAsp: 3.761 ± 0.073	3.886IleGlu: 3.886 ± 0.071	2.634IlePhe: 2.634 ± 0.069	4.812IleGly: 4.812 ± 0.094	1.2IleHis: 1.2 ± 0.043	3.955IleIle: 3.955 ± 0.089	3.119IleLys: 3.119 ± 0.071	6.25IleLeu: 6.25 ± 0.108	1.457IleMet: 1.457 ± 0.043	2.362IleAsn: 2.362 ± 0.065	3.151IlePro: 3.151 ± 0.074	2.337IleGln: 2.337 ± 0.061	3.395IleArg: 3.395 ± 0.074	4.623IleSer: 4.623 ± 0.078	3.694IleThr: 3.694 ± 0.079	4.377IleVal: 4.377 ± 0.085	0.585IleTrp: 0.585 ± 0.031	2.344IleTyr: 2.344 ± 0.055	0.001IleXaa: 0.001 ± 0.001
Lys	5.337LysAla: 5.337 ± 0.09	0.61LysCys: 0.61 ± 0.03	2.993LysAsp: 2.993 ± 0.069	5.038LysGlu: 5.038 ± 0.092	1.613LysPhe: 1.613 ± 0.051	3.897LysGly: 3.897 ± 0.069	0.865LysHis: 0.865 ± 0.037	4.103LysIle: 4.103 ± 0.07	4.627LysLys: 4.627 ± 0.092	5.195LysLeu: 5.195 ± 0.083	1.751LysMet: 1.751 ± 0.048	2.834LysAsn: 2.834 ± 0.066	2.245LysPro: 2.245 ± 0.062	1.895LysGln: 1.895 ± 0.048	3.272LysArg: 3.272 ± 0.067	3.063LysSer: 3.063 ± 0.063	3.202LysThr: 3.202 ± 0.062	3.466LysVal: 3.466 ± 0.071	0.492LysTrp: 0.492 ± 0.029	2.027LysTyr: 2.027 ± 0.055	0.001LysXaa: 0.001 ± 0.001
Leu	7.832LeuAla: 7.832 ± 0.112	1.867LeuCys: 1.867 ± 0.052	5.037LeuAsp: 5.037 ± 0.09	6.805LeuGlu: 6.805 ± 0.112	4.184LeuPhe: 4.184 ± 0.092	6.432LeuGly: 6.432 ± 0.088	1.468LeuHis: 1.468 ± 0.048	5.685LeuIle: 5.685 ± 0.101	5.851LeuLys: 5.851 ± 0.093	9.545LeuLeu: 9.545 ± 0.153	2.587LeuMet: 2.587 ± 0.06	3.562LeuAsn: 3.562 ± 0.074	4.449LeuPro: 4.449 ± 0.081	3.084LeuGln: 3.084 ± 0.066	4.698LeuArg: 4.698 ± 0.091	7.001LeuSer: 7.001 ± 0.108	5.279LeuThr: 5.279 ± 0.084	5.921LeuVal: 5.921 ± 0.1	0.965LeuTrp: 0.965 ± 0.039	3.277LeuTyr: 3.277 ± 0.066	0.001LeuXaa: 0.001 ± 0.001
Met	2.538MetAla: 2.538 ± 0.067	0.327MetCys: 0.327 ± 0.02	1.594MetAsp: 1.594 ± 0.047	2.213MetGlu: 2.213 ± 0.054	0.994MetPhe: 0.994 ± 0.038	2.063MetGly: 2.063 ± 0.053	0.357MetHis: 0.357 ± 0.022	1.826MetIle: 1.826 ± 0.05	2.177MetLys: 2.177 ± 0.049	2.55MetLeu: 2.55 ± 0.069	0.872MetMet: 0.872 ± 0.035	1.258MetAsn: 1.258 ± 0.04	1.184MetPro: 1.184 ± 0.04	0.806MetGln: 0.806 ± 0.033	1.297MetArg: 1.297 ± 0.043	1.576MetSer: 1.576 ± 0.047	1.651MetThr: 1.651 ± 0.045	1.913MetVal: 1.913 ± 0.056	0.223MetTrp: 0.223 ± 0.018	0.687MetTyr: 0.687 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.024AsnAla: 3.024 ± 0.063	0.538AsnCys: 0.538 ± 0.03	1.865AsnAsp: 1.865 ± 0.055	2.151AsnGlu: 2.151 ± 0.054	1.482AsnPhe: 1.482 ± 0.045	3.455AsnGly: 3.455 ± 0.074	0.747AsnHis: 0.747 ± 0.032	2.572AsnIle: 2.572 ± 0.07	1.728AsnLys: 1.728 ± 0.05	3.586AsnLeu: 3.586 ± 0.065	1.03AsnMet: 1.03 ± 0.037	1.439AsnAsn: 1.439 ± 0.052	2.148AsnPro: 2.148 ± 0.06	1.641AsnGln: 1.641 ± 0.039	2.011AsnArg: 2.011 ± 0.054	2.267AsnSer: 2.267 ± 0.063	2.049AsnThr: 2.049 ± 0.05	2.539AsnVal: 2.539 ± 0.055	0.432AsnTrp: 0.432 ± 0.027	1.504AsnTyr: 1.504 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.388ProAla: 3.388 ± 0.076	0.584ProCys: 0.584 ± 0.028	2.592ProAsp: 2.592 ± 0.063	3.936ProGlu: 3.936 ± 0.076	1.768ProPhe: 1.768 ± 0.054	2.957ProGly: 2.957 ± 0.066	0.638ProHis: 0.638 ± 0.031	2.212ProIle: 2.212 ± 0.05	2.095ProLys: 2.095 ± 0.054	3.41ProLeu: 3.41 ± 0.065	1.079ProMet: 1.079 ± 0.039	1.435ProAsn: 1.435 ± 0.044	1.25ProPro: 1.25 ± 0.051	1.583ProGln: 1.583 ± 0.053	1.438ProArg: 1.438 ± 0.043	2.194ProSer: 2.194 ± 0.053	1.72ProThr: 1.72 ± 0.052	3.476ProVal: 3.476 ± 0.07	0.383ProTrp: 0.383 ± 0.024	1.581ProTyr: 1.581 ± 0.051	0.003ProXaa: 0.003 ± 0.002
Gln	3.353GlnAla: 3.353 ± 0.053	0.461GlnCys: 0.461 ± 0.025	1.589GlnAsp: 1.589 ± 0.047	3.142GlnGlu: 3.142 ± 0.068	1.297GlnPhe: 1.297 ± 0.045	2.455GlnGly: 2.455 ± 0.059	0.472GlnHis: 0.472 ± 0.025	2.462GlnIle: 2.462 ± 0.063	2.754GlnLys: 2.754 ± 0.061	2.944GlnLeu: 2.944 ± 0.065	1.13GlnMet: 1.13 ± 0.039	1.638GlnAsn: 1.638 ± 0.049	1.373GlnPro: 1.373 ± 0.046	1.416GlnGln: 1.416 ± 0.048	1.933GlnArg: 1.933 ± 0.052	1.98GlnSer: 1.98 ± 0.048	1.794GlnThr: 1.794 ± 0.051	2.528GlnVal: 2.528 ± 0.065	0.461GlnTrp: 0.461 ± 0.028	1.387GlnTyr: 1.387 ± 0.049	0.001GlnXaa: 0.001 ± 0.001
Arg	3.248ArgAla: 3.248 ± 0.073	0.762ArgCys: 0.762 ± 0.036	2.39ArgAsp: 2.39 ± 0.058	4.176ArgGlu: 4.176 ± 0.085	2.257ArgPhe: 2.257 ± 0.063	2.892ArgGly: 2.892 ± 0.068	0.954ArgHis: 0.954 ± 0.037	3.462ArgIle: 3.462 ± 0.072	3.246ArgLys: 3.246 ± 0.075	5.305ArgLeu: 5.305 ± 0.087	1.536ArgMet: 1.536 ± 0.047	1.892ArgAsn: 1.892 ± 0.056	1.792ArgPro: 1.792 ± 0.063	2.002ArgGln: 2.002 ± 0.056	3.067ArgArg: 3.067 ± 0.065	2.471ArgSer: 2.471 ± 0.066	2.278ArgThr: 2.278 ± 0.053	3.162ArgVal: 3.162 ± 0.073	0.506ArgTrp: 0.506 ± 0.029	2.016ArgTyr: 2.016 ± 0.048	0.001ArgXaa: 0.001 ± 0.001
Ser	4.942SerAla: 4.942 ± 0.086	0.965SerCys: 0.965 ± 0.04	3.092SerAsp: 3.092 ± 0.069	3.584SerGlu: 3.584 ± 0.08	3.005SerPhe: 3.005 ± 0.068	5.652SerGly: 5.652 ± 0.111	1.053SerHis: 1.053 ± 0.04	3.824SerIle: 3.824 ± 0.081	2.935SerLys: 2.935 ± 0.068	6.017SerLeu: 6.017 ± 0.088	1.657SerMet: 1.657 ± 0.044	2.006SerAsn: 2.006 ± 0.059	2.308SerPro: 2.308 ± 0.056	2.507SerGln: 2.507 ± 0.063	3.144SerArg: 3.144 ± 0.074	4.407SerSer: 4.407 ± 0.124	2.812SerThr: 2.812 ± 0.065	4.535SerVal: 4.535 ± 0.073	0.65SerTrp: 0.65 ± 0.032	2.311SerTyr: 2.311 ± 0.059	0.001SerXaa: 0.001 ± 0.001
Thr	4.792ThrAla: 4.792 ± 0.092	0.608ThrCys: 0.608 ± 0.029	2.795ThrAsp: 2.795 ± 0.067	3.294ThrGlu: 3.294 ± 0.07	1.943ThrPhe: 1.943 ± 0.051	4.893ThrGly: 4.893 ± 0.107	0.828ThrHis: 0.828 ± 0.037	3.329ThrIle: 3.329 ± 0.067	2.459ThrLys: 2.459 ± 0.058	4.927ThrLeu: 4.927 ± 0.09	1.319ThrMet: 1.319 ± 0.037	1.688ThrAsn: 1.688 ± 0.053	2.486ThrPro: 2.486 ± 0.056	1.633ThrGln: 1.633 ± 0.044	1.954ThrArg: 1.954 ± 0.046	2.811ThrSer: 2.811 ± 0.071	2.511ThrThr: 2.511 ± 0.075	4.719ThrVal: 4.719 ± 0.096	0.5ThrTrp: 0.5 ± 0.024	1.746ThrTyr: 1.746 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	5.134ValAla: 5.134 ± 0.097	1.378ValCys: 1.378 ± 0.048	3.708ValAsp: 3.708 ± 0.076	4.605ValGlu: 4.605 ± 0.083	3.222ValPhe: 3.222 ± 0.064	4.328ValGly: 4.328 ± 0.083	1.03ValHis: 1.03 ± 0.036	4.849ValIle: 4.849 ± 0.086	4.178ValLys: 4.178 ± 0.08	7.162ValLeu: 7.162 ± 0.102	2.124ValMet: 2.124 ± 0.055	2.718ValAsn: 2.718 ± 0.068	2.883ValPro: 2.883 ± 0.062	2.058ValGln: 2.058 ± 0.055	3.197ValArg: 3.197 ± 0.068	5.019ValSer: 5.019 ± 0.07	4.207ValThr: 4.207 ± 0.093	5.014ValVal: 5.014 ± 0.096	0.734ValTrp: 0.734 ± 0.032	2.569ValTyr: 2.569 ± 0.066	0.001ValXaa: 0.001 ± 0.001
Trp	0.717TrpAla: 0.717 ± 0.037	0.189TrpCys: 0.189 ± 0.017	0.635TrpAsp: 0.635 ± 0.031	0.74TrpGlu: 0.74 ± 0.039	0.492TrpPhe: 0.492 ± 0.028	0.756TrpGly: 0.756 ± 0.033	0.198TrpHis: 0.198 ± 0.018	0.621TrpIle: 0.621 ± 0.035	0.657TrpLys: 0.657 ± 0.032	1.037TrpLeu: 1.037 ± 0.042	0.298TrpMet: 0.298 ± 0.019	0.593TrpAsn: 0.593 ± 0.031	0.302TrpPro: 0.302 ± 0.02	0.463TrpGln: 0.463 ± 0.03	0.48TrpArg: 0.48 ± 0.026	0.568TrpSer: 0.568 ± 0.028	0.503TrpThr: 0.503 ± 0.024	0.567TrpVal: 0.567 ± 0.028	0.144TrpTrp: 0.144 ± 0.016	0.414TrpTyr: 0.414 ± 0.027	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.626TyrAla: 2.626 ± 0.054	0.661TyrCys: 0.661 ± 0.026	2.308TyrAsp: 2.308 ± 0.063	2.398TyrGlu: 2.398 ± 0.064	1.822TyrPhe: 1.822 ± 0.052	3.012TyrGly: 3.012 ± 0.064	0.905TyrHis: 0.905 ± 0.037	2.015TyrIle: 2.015 ± 0.054	1.444TyrLys: 1.444 ± 0.045	3.809TyrLeu: 3.809 ± 0.074	0.804TyrMet: 0.804 ± 0.036	1.407TyrAsn: 1.407 ± 0.047	1.679TyrPro: 1.679 ± 0.048	1.888TyrGln: 1.888 ± 0.057	2.244TyrArg: 2.244 ± 0.054	2.296TyrSer: 2.296 ± 0.058	1.987TyrThr: 1.987 ± 0.06	2.23TyrVal: 2.23 ± 0.053	0.415TyrTrp: 0.415 ± 0.026	1.664TyrTyr: 1.664 ± 0.053	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.005XaaGly: 0.005 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.003XaaThr: 0.003 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.021XaaXaa: 0.021 ± 0.007

Statistics based on 2482 proteins (772695 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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