Meleagrid herpesvirus 1 (MeHV-1) (Turkey herpesvirus)
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DPP7|Q9DPP7_MEHV1 Uncharacterized protein HVT068 OS=Meleagrid herpesvirus 1 OX=37108 GN=HVT068 PE=4 SV=1
MM1 pKa = 7.82 DD2 pKa = 5.16 AEE4 pKa = 4.67 RR5 pKa = 11.84 PPGDD9 pKa = 4.22 PEE11 pKa = 4.85 NGLPLTTLEE20 pKa = 4.3 ARR22 pKa = 11.84 HH23 pKa = 6.4 DD24 pKa = 4.14 RR25 pKa = 11.84 DD26 pKa = 3.96 SNSAPIEE33 pKa = 4.12 EE34 pKa = 4.42 VAGDD38 pKa = 4.12 IPAPSPPEE46 pKa = 3.91 YY47 pKa = 10.5 LALTLSEE54 pKa = 4.54 AEE56 pKa = 4.75 SIFEE60 pKa = 4.08 PRR62 pKa = 11.84 PARR65 pKa = 11.84 GPEE68 pKa = 4.19 GDD70 pKa = 3.71 PHH72 pKa = 6.34 PRR74 pKa = 11.84 LYY76 pKa = 10.41 PPLPPPNYY84 pKa = 9.06 EE85 pKa = 4.49 DD86 pKa = 5.74 LFAEE90 pKa = 5.13 DD91 pKa = 4.42 PTPHH95 pKa = 6.93 RR96 pKa = 11.84 PYY98 pKa = 10.61 DD99 pKa = 3.49 IASDD103 pKa = 3.58 AVAIAILGVAFVGTVLSVVVLLVCSKK129 pKa = 11.22
Molecular weight: 13.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.29
IPC2_protein 4.24
IPC_protein 4.164
Toseland 3.986
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.012
Rodwell 3.999
Grimsley 3.897
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.024
Sillero 4.279
Patrickios 3.528
IPC_peptide 4.126
IPC2_peptide 4.266
IPC2.peptide.svr19 4.204
Protein with the highest isoelectric point:
>tr|Q9DH38|Q9DH38_MEHV1 UL46 tegument phosphoprotein OS=Meleagrid herpesvirus 1 OX=37108 GN=HVT054 PE=3 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 ITHH5 pKa = 6.28 TLDD8 pKa = 3.31 FSGSLGGTILINAAVYY24 pKa = 8.82 QRR26 pKa = 11.84 RR27 pKa = 11.84 GLVAGGQGYY36 pKa = 7.32 QRR38 pKa = 11.84 RR39 pKa = 11.84 ARR41 pKa = 11.84 CSSLLADD48 pKa = 3.85 TGDD51 pKa = 3.86 PNEE54 pKa = 4.35 PLMPARR60 pKa = 11.84 AVLTHH65 pKa = 6.54 PPCSRR70 pKa = 11.84 NHH72 pKa = 6.06 SPCSSARR79 pKa = 11.84 YY80 pKa = 8.31 AASAAAKK87 pKa = 10.02 RR88 pKa = 11.84 LGPSSQPFSYY98 pKa = 10.39 SAVASEE104 pKa = 4.55 PPRR107 pKa = 11.84 TMVARR112 pKa = 11.84 HH113 pKa = 5.78 RR114 pKa = 11.84 PQLKK118 pKa = 9.53 PLPEE122 pKa = 4.45 FNSDD126 pKa = 3.29 TTLCSADD133 pKa = 3.36 NAACVPPLANGAQEE147 pKa = 4.19 RR148 pKa = 11.84 KK149 pKa = 9.57 KK150 pKa = 10.6 GLSRR154 pKa = 11.84 RR155 pKa = 11.84 SSSAAARR162 pKa = 11.84 RR163 pKa = 11.84 SSAAVGLGGGPSLPQQCWKK182 pKa = 10.87
Molecular weight: 19.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.619
IPC_protein 10.54
Toseland 10.555
ProMoST 10.409
Dawson 10.687
Bjellqvist 10.467
Wikipedia 10.921
Rodwell 10.774
Grimsley 10.745
Solomon 10.818
Lehninger 10.774
Nozaki 10.613
DTASelect 10.438
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.496
IPC_peptide 10.818
IPC2_peptide 9.867
IPC2.peptide.svr19 8.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
41221
69
3112
473.8
52.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.894 ± 0.219
2.484 ± 0.139
5.548 ± 0.162
5.172 ± 0.162
3.85 ± 0.181
5.963 ± 0.16
2.457 ± 0.083
6.101 ± 0.212
4.013 ± 0.146
9.182 ± 0.228
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.089
4.187 ± 0.138
5.235 ± 0.313
2.627 ± 0.096
6.776 ± 0.196
8.581 ± 0.223
6.434 ± 0.152
6.128 ± 0.127
1.116 ± 0.066
3.607 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here