Mucilaginibacter yixingensis
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4440 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T5JEF2|A0A2T5JEF2_9SPHI RNA polymerase ECF family sigma subunit OS=Mucilaginibacter yixingensis OX=1295612 GN=C8P68_10122 PE=3 SV=1
MM1 pKa = 7.7 PNIKK5 pKa = 10.24 LSYY8 pKa = 9.91 FYY10 pKa = 10.52 RR11 pKa = 11.84 DD12 pKa = 3.06 SANYY16 pKa = 9.96 KK17 pKa = 9.59 VFNNVIFFNDD27 pKa = 3.09 QGLDD31 pKa = 3.56 LVSVEE36 pKa = 5.61 DD37 pKa = 4.16 IIKK40 pKa = 10.71 SKK42 pKa = 11.02 LIDD45 pKa = 3.5 QTWFYY50 pKa = 10.21 PGEE53 pKa = 3.84 WHH55 pKa = 7.52 LPDD58 pKa = 4.94 LFSGYY63 pKa = 9.99 FDD65 pKa = 5.59 PNIDD69 pKa = 4.0 PAWHH73 pKa = 6.18 EE74 pKa = 4.39 FEE76 pKa = 4.6 SLDD79 pKa = 4.83 YY80 pKa = 11.24 SDD82 pKa = 6.06 EE83 pKa = 4.95 DD84 pKa = 3.92 LLCDD88 pKa = 3.15 IRR90 pKa = 11.84 LSVFLNNII98 pKa = 3.41
Molecular weight: 11.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 4.012
IPC_protein 3.973
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.961
Lehninger 3.923
Nozaki 4.101
DTASelect 4.355
Thurlkill 3.821
EMBOSS 3.935
Sillero 4.101
Patrickios 1.952
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.988
Protein with the highest isoelectric point:
>tr|A0A2T5JEB2|A0A2T5JEB2_9SPHI Helix-turn-helix protein OS=Mucilaginibacter yixingensis OX=1295612 GN=C8P68_1019 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.39 KK6 pKa = 9.62 RR7 pKa = 11.84 KK8 pKa = 6.72 RR9 pKa = 11.84 HH10 pKa = 5.17 KK11 pKa = 10.05 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4440
0
4440
1570352
29
3133
353.7
39.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.167 ± 0.033
0.844 ± 0.011
5.548 ± 0.027
5.058 ± 0.04
4.661 ± 0.024
7.074 ± 0.037
2.05 ± 0.017
6.675 ± 0.033
6.37 ± 0.031
9.363 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.017
5.484 ± 0.035
4.063 ± 0.027
4.034 ± 0.024
4.172 ± 0.024
6.028 ± 0.032
6.056 ± 0.043
6.528 ± 0.027
1.255 ± 0.015
4.218 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here