Sodalis-like symbiont of Bactericera trigonica
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1308 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L7LSW5|A0A3L7LSW5_9GAMM Tail fiber domain-containing protein OS=Sodalis-like symbiont of Bactericera trigonica OX=2420513 GN=D8L93_03515 PE=4 SV=1
MM1 pKa = 7.59 SEE3 pKa = 3.98 QNNTEE8 pKa = 3.55 IAFQIQRR15 pKa = 11.84 IYY17 pKa = 10.13 TKK19 pKa = 10.3 DD20 pKa = 3.43 VSFEE24 pKa = 4.02 APNAPKK30 pKa = 10.45 VFQQEE35 pKa = 3.95 WQPEE39 pKa = 4.07 IKK41 pKa = 10.36 LDD43 pKa = 3.84 LDD45 pKa = 3.92 TASSQLAEE53 pKa = 5.2 DD54 pKa = 3.5 IYY56 pKa = 11.2 EE57 pKa = 4.09 VVLRR61 pKa = 11.84 VTVTATLGEE70 pKa = 4.28 DD71 pKa = 3.3 TAFLCEE77 pKa = 4.07 VQQAGIFTISGIDD90 pKa = 3.54 STQMAHH96 pKa = 7.17 CLGAYY101 pKa = 9.19 CPNILFSYY109 pKa = 10.31 ARR111 pKa = 11.84 EE112 pKa = 4.45 CITSQVSRR120 pKa = 11.84 GTFPQLNLAPVNFDD134 pKa = 3.79 TLFMNYY140 pKa = 9.3 LQQQAAGVGSQPXQDD155 pKa = 4.1 DD156 pKa = 3.37
Molecular weight: 17.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.835
IPC2_protein 4.088
IPC_protein 4.012
Toseland 3.821
ProMoST 4.075
Dawson 3.973
Bjellqvist 4.177
Wikipedia 3.884
Rodwell 3.846
Grimsley 3.732
Solomon 3.961
Lehninger 3.923
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.126
Patrickios 0.947
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.045
Protein with the highest isoelectric point:
>tr|A0A3L7LS07|A0A3L7LS07_9GAMM Imidazole glycerol phosphate synthase subunit HisH OS=Sodalis-like symbiont of Bactericera trigonica OX=2420513 GN=hisH PE=3 SV=1
MM1 pKa = 7.29 ATQARR6 pKa = 11.84 QSWPRR11 pKa = 11.84 TSPTGDD17 pKa = 3.03 AARR20 pKa = 11.84 NNVCLFVANDD30 pKa = 4.07 LEE32 pKa = 4.69 YY33 pKa = 10.24 PLPDD37 pKa = 2.99 HH38 pKa = 6.53 VFRR41 pKa = 11.84 GTLLPTPLSPWCRR54 pKa = 11.84 VANVRR59 pKa = 11.84 QGVLVLEE66 pKa = 4.48 TANASWKK73 pKa = 9.02 MRR75 pKa = 11.84 LRR77 pKa = 11.84 YY78 pKa = 7.44 EE79 pKa = 4.17 QPRR82 pKa = 11.84 LLSALRR88 pKa = 11.84 AQILPSLSSIDD99 pKa = 3.36 IRR101 pKa = 11.84 INPSMARR108 pKa = 11.84 KK109 pKa = 9.49 QEE111 pKa = 4.16 LNAQNKK117 pKa = 10.12 DD118 pKa = 2.89 SGQQLDD124 pKa = 4.15 RR125 pKa = 11.84 PPGEE129 pKa = 3.82 KK130 pKa = 9.48 PRR132 pKa = 11.84 RR133 pKa = 11.84 LSVQSAASIRR143 pKa = 11.84 HH144 pKa = 3.98 VAEE147 pKa = 3.98 RR148 pKa = 11.84 SEE150 pKa = 4.52 GKK152 pKa = 10.12 LKK154 pKa = 10.85 NALEE158 pKa = 4.21 RR159 pKa = 11.84 LAEE162 pKa = 3.99 LASRR166 pKa = 11.84 RR167 pKa = 11.84 EE168 pKa = 4.18 CQPRR172 pKa = 11.84 PP173 pKa = 3.39
Molecular weight: 19.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.487
IPC_protein 10.409
Toseland 10.76
ProMoST 10.555
Dawson 10.818
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 10.891
Grimsley 10.847
Solomon 11.008
Lehninger 10.979
Nozaki 10.76
DTASelect 10.57
Thurlkill 10.745
EMBOSS 11.169
Sillero 10.76
Patrickios 10.643
IPC_peptide 11.023
IPC2_peptide 9.809
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1308
0
1308
230015
21
1271
175.9
19.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.305 ± 0.096
1.244 ± 0.028
5.311 ± 0.053
5.441 ± 0.077
3.462 ± 0.05
7.386 ± 0.081
2.419 ± 0.038
5.485 ± 0.065
3.964 ± 0.072
11.135 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.742 ± 0.042
3.358 ± 0.044
4.509 ± 0.048
4.606 ± 0.069
6.863 ± 0.067
5.623 ± 0.063
5.091 ± 0.058
6.956 ± 0.075
1.327 ± 0.037
2.739 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here