Amino acid dipepetide frequency for Gordonia phage Aphelion

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.228AlaAla: 10.228 ± 0.906
0.618AlaCys: 0.618 ± 0.149
7.105AlaAsp: 7.105 ± 0.433
6.556AlaGlu: 6.556 ± 0.587
2.78AlaPhe: 2.78 ± 0.447
7.482AlaGly: 7.482 ± 0.612
2.128AlaHis: 2.128 ± 0.328
4.428AlaIle: 4.428 ± 0.489
3.947AlaLys: 3.947 ± 0.565
7.963AlaLeu: 7.963 ± 0.485
2.471AlaMet: 2.471 ± 0.257
3.261AlaAsn: 3.261 ± 0.314
4.325AlaPro: 4.325 ± 0.405
4.737AlaGln: 4.737 ± 0.705
6.693AlaArg: 6.693 ± 0.644
4.805AlaSer: 4.805 ± 0.356
5.045AlaThr: 5.045 ± 0.438
7.002AlaVal: 7.002 ± 0.506
1.888AlaTrp: 1.888 ± 0.268
2.265AlaTyr: 2.265 ± 0.253
0.0AlaXaa: 0.0 ± 0.0
Cys
0.755CysAla: 0.755 ± 0.181
0.24CysCys: 0.24 ± 0.106
1.167CysAsp: 1.167 ± 0.227
0.721CysGlu: 0.721 ± 0.164
0.378CysPhe: 0.378 ± 0.104
1.339CysGly: 1.339 ± 0.266
0.378CysHis: 0.378 ± 0.128
0.378CysIle: 0.378 ± 0.131
0.549CysLys: 0.549 ± 0.124
0.515CysLeu: 0.515 ± 0.127
0.0CysMet: 0.0 ± 0.0
0.378CysAsn: 0.378 ± 0.115
0.549CysPro: 0.549 ± 0.153
0.446CysGln: 0.446 ± 0.111
0.755CysArg: 0.755 ± 0.17
0.412CysSer: 0.412 ± 0.112
0.515CysThr: 0.515 ± 0.129
0.858CysVal: 0.858 ± 0.214
0.137CysTrp: 0.137 ± 0.064
0.137CysTyr: 0.137 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
6.212AspAla: 6.212 ± 0.489
0.755AspCys: 0.755 ± 0.183
5.183AspAsp: 5.183 ± 0.522
5.08AspGlu: 5.08 ± 0.474
2.609AspPhe: 2.609 ± 0.297
5.972AspGly: 5.972 ± 0.51
1.785AspHis: 1.785 ± 0.252
3.673AspIle: 3.673 ± 0.404
2.437AspLys: 2.437 ± 0.342
6.007AspLeu: 6.007 ± 0.363
1.888AspMet: 1.888 ± 0.266
2.162AspAsn: 2.162 ± 0.328
4.359AspPro: 4.359 ± 0.44
2.265AspGln: 2.265 ± 0.289
5.011AspArg: 5.011 ± 0.449
2.917AspSer: 2.917 ± 0.345
4.393AspThr: 4.393 ± 0.356
5.114AspVal: 5.114 ± 0.395
1.304AspTrp: 1.304 ± 0.266
2.78AspTyr: 2.78 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
6.899GluAla: 6.899 ± 0.772
0.755GluCys: 0.755 ± 0.175
3.947GluAsp: 3.947 ± 0.357
4.256GluGlu: 4.256 ± 0.38
2.471GluPhe: 2.471 ± 0.278
4.84GluGly: 4.84 ± 0.326
1.27GluHis: 1.27 ± 0.188
4.016GluIle: 4.016 ± 0.327
2.677GluLys: 2.677 ± 0.394
6.521GluLeu: 6.521 ± 0.466
2.197GluMet: 2.197 ± 0.244
1.991GluAsn: 1.991 ± 0.226
3.192GluPro: 3.192 ± 0.529
2.54GluGln: 2.54 ± 0.315
4.187GluArg: 4.187 ± 0.393
3.261GluSer: 3.261 ± 0.332
3.638GluThr: 3.638 ± 0.377
4.634GluVal: 4.634 ± 0.505
2.094GluTrp: 2.094 ± 0.298
2.334GluTyr: 2.334 ± 0.295
0.0GluXaa: 0.0 ± 0.0
Phe
3.364PheAla: 3.364 ± 0.296
0.378PheCys: 0.378 ± 0.134
3.055PheAsp: 3.055 ± 0.327
2.334PheGlu: 2.334 ± 0.322
0.583PhePhe: 0.583 ± 0.16
2.3PheGly: 2.3 ± 0.309
0.686PheHis: 0.686 ± 0.121
1.442PheIle: 1.442 ± 0.21
1.098PheLys: 1.098 ± 0.178
2.162PheLeu: 2.162 ± 0.299
1.03PheMet: 1.03 ± 0.207
1.51PheAsn: 1.51 ± 0.196
1.442PhePro: 1.442 ± 0.232
0.789PheGln: 0.789 ± 0.174
1.545PheArg: 1.545 ± 0.231
2.128PheSer: 2.128 ± 0.264
2.265PheThr: 2.265 ± 0.288
2.265PheVal: 2.265 ± 0.318
0.824PheTrp: 0.824 ± 0.169
0.755PheTyr: 0.755 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
5.835GlyAla: 5.835 ± 0.513
0.824GlyCys: 0.824 ± 0.18
5.32GlyAsp: 5.32 ± 0.52
5.114GlyGlu: 5.114 ± 0.412
2.712GlyPhe: 2.712 ± 0.35
6.418GlyGly: 6.418 ± 0.752
2.025GlyHis: 2.025 ± 0.318
3.947GlyIle: 3.947 ± 0.38
3.673GlyLys: 3.673 ± 0.376
6.075GlyLeu: 6.075 ± 0.542
2.162GlyMet: 2.162 ± 0.256
2.746GlyAsn: 2.746 ± 0.367
3.81GlyPro: 3.81 ± 0.377
3.055GlyGln: 3.055 ± 0.341
5.045GlyArg: 5.045 ± 0.344
4.634GlySer: 4.634 ± 0.386
4.702GlyThr: 4.702 ± 0.449
6.384GlyVal: 6.384 ± 0.404
2.265GlyTrp: 2.265 ± 0.31
2.3GlyTyr: 2.3 ± 0.303
0.0GlyXaa: 0.0 ± 0.0
His
1.648HisAla: 1.648 ± 0.257
0.275HisCys: 0.275 ± 0.093
1.133HisAsp: 1.133 ± 0.247
1.51HisGlu: 1.51 ± 0.241
0.995HisPhe: 0.995 ± 0.184
2.128HisGly: 2.128 ± 0.296
0.549HisHis: 0.549 ± 0.139
1.098HisIle: 1.098 ± 0.214
0.618HisLys: 0.618 ± 0.119
2.162HisLeu: 2.162 ± 0.292
0.446HisMet: 0.446 ± 0.111
0.858HisAsn: 0.858 ± 0.175
1.442HisPro: 1.442 ± 0.252
0.824HisGln: 0.824 ± 0.199
1.819HisArg: 1.819 ± 0.285
1.27HisSer: 1.27 ± 0.207
1.304HisThr: 1.304 ± 0.191
1.991HisVal: 1.991 ± 0.317
0.481HisTrp: 0.481 ± 0.136
0.858HisTyr: 0.858 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
4.908IleAla: 4.908 ± 0.402
0.583IleCys: 0.583 ± 0.142
4.153IleAsp: 4.153 ± 0.424
3.776IleGlu: 3.776 ± 0.351
0.961IlePhe: 0.961 ± 0.16
3.055IleGly: 3.055 ± 0.278
0.995IleHis: 0.995 ± 0.167
2.094IleIle: 2.094 ± 0.314
2.094IleLys: 2.094 ± 0.294
3.329IleLeu: 3.329 ± 0.363
0.481IleMet: 0.481 ± 0.106
1.819IleAsn: 1.819 ± 0.287
2.471IlePro: 2.471 ± 0.302
1.922IleGln: 1.922 ± 0.259
3.81IleArg: 3.81 ± 0.35
3.055IleSer: 3.055 ± 0.383
3.261IleThr: 3.261 ± 0.3
2.883IleVal: 2.883 ± 0.368
1.098IleTrp: 1.098 ± 0.18
1.27IleTyr: 1.27 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
3.432LysAla: 3.432 ± 0.369
0.652LysCys: 0.652 ± 0.186
2.609LysAsp: 2.609 ± 0.337
2.128LysGlu: 2.128 ± 0.297
1.579LysPhe: 1.579 ± 0.285
3.123LysGly: 3.123 ± 0.398
0.927LysHis: 0.927 ± 0.171
1.648LysIle: 1.648 ± 0.216
2.265LysLys: 2.265 ± 0.356
3.776LysLeu: 3.776 ± 0.404
1.098LysMet: 1.098 ± 0.215
1.304LysAsn: 1.304 ± 0.215
2.368LysPro: 2.368 ± 0.321
1.236LysGln: 1.236 ± 0.226
2.952LysArg: 2.952 ± 0.346
2.368LysSer: 2.368 ± 0.323
1.888LysThr: 1.888 ± 0.257
3.844LysVal: 3.844 ± 0.379
0.721LysTrp: 0.721 ± 0.159
1.064LysTyr: 1.064 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
6.968LeuAla: 6.968 ± 0.68
0.961LeuCys: 0.961 ± 0.206
5.869LeuAsp: 5.869 ± 0.414
6.109LeuGlu: 6.109 ± 0.451
2.162LeuPhe: 2.162 ± 0.239
6.041LeuGly: 6.041 ± 0.54
1.888LeuHis: 1.888 ± 0.254
3.535LeuIle: 3.535 ± 0.268
2.712LeuLys: 2.712 ± 0.331
5.114LeuLeu: 5.114 ± 0.46
1.648LeuMet: 1.648 ± 0.226
3.501LeuAsn: 3.501 ± 0.382
3.947LeuPro: 3.947 ± 0.481
3.02LeuGln: 3.02 ± 0.309
5.354LeuArg: 5.354 ± 0.368
4.153LeuSer: 4.153 ± 0.364
5.011LeuThr: 5.011 ± 0.411
4.908LeuVal: 4.908 ± 0.441
1.236LeuTrp: 1.236 ± 0.223
2.025LeuTyr: 2.025 ± 0.221
0.0LeuXaa: 0.0 ± 0.0
Met
2.883MetAla: 2.883 ± 0.288
0.172MetCys: 0.172 ± 0.073
1.373MetAsp: 1.373 ± 0.232
1.27MetGlu: 1.27 ± 0.199
0.789MetPhe: 0.789 ± 0.141
1.545MetGly: 1.545 ± 0.185
0.618MetHis: 0.618 ± 0.169
1.167MetIle: 1.167 ± 0.237
0.824MetLys: 0.824 ± 0.175
1.682MetLeu: 1.682 ± 0.233
0.343MetMet: 0.343 ± 0.124
1.133MetAsn: 1.133 ± 0.194
1.407MetPro: 1.407 ± 0.201
0.995MetGln: 0.995 ± 0.196
1.819MetArg: 1.819 ± 0.271
2.677MetSer: 2.677 ± 0.291
2.162MetThr: 2.162 ± 0.283
1.51MetVal: 1.51 ± 0.244
0.378MetTrp: 0.378 ± 0.119
0.343MetTyr: 0.343 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
3.707AsnAla: 3.707 ± 0.414
0.618AsnCys: 0.618 ± 0.164
2.265AsnAsp: 2.265 ± 0.218
2.54AsnGlu: 2.54 ± 0.26
0.892AsnPhe: 0.892 ± 0.201
3.535AsnGly: 3.535 ± 0.301
0.892AsnHis: 0.892 ± 0.159
1.407AsnIle: 1.407 ± 0.253
1.545AsnLys: 1.545 ± 0.288
3.226AsnLeu: 3.226 ± 0.332
0.549AsnMet: 0.549 ± 0.162
1.27AsnAsn: 1.27 ± 0.25
1.888AsnPro: 1.888 ± 0.237
1.339AsnGln: 1.339 ± 0.214
2.403AsnArg: 2.403 ± 0.308
2.059AsnSer: 2.059 ± 0.201
2.059AsnThr: 2.059 ± 0.355
2.368AsnVal: 2.368 ± 0.303
0.618AsnTrp: 0.618 ± 0.138
0.927AsnTyr: 0.927 ± 0.168
0.0AsnXaa: 0.0 ± 0.0
Pro
5.114ProAla: 5.114 ± 0.462
0.378ProCys: 0.378 ± 0.12
3.913ProAsp: 3.913 ± 0.423
3.673ProGlu: 3.673 ± 0.419
1.819ProPhe: 1.819 ± 0.277
4.599ProGly: 4.599 ± 0.368
0.892ProHis: 0.892 ± 0.2
2.231ProIle: 2.231 ± 0.267
2.265ProLys: 2.265 ± 0.311
2.78ProLeu: 2.78 ± 0.305
0.927ProMet: 0.927 ± 0.203
1.648ProAsn: 1.648 ± 0.22
1.956ProPro: 1.956 ± 0.27
1.545ProGln: 1.545 ± 0.234
2.952ProArg: 2.952 ± 0.323
2.746ProSer: 2.746 ± 0.349
3.123ProThr: 3.123 ± 0.454
3.432ProVal: 3.432 ± 0.419
1.03ProTrp: 1.03 ± 0.177
1.579ProTyr: 1.579 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
3.467GlnAla: 3.467 ± 0.442
0.378GlnCys: 0.378 ± 0.113
1.853GlnAsp: 1.853 ± 0.276
2.059GlnGlu: 2.059 ± 0.309
0.961GlnPhe: 0.961 ± 0.197
2.643GlnGly: 2.643 ± 0.243
0.858GlnHis: 0.858 ± 0.181
1.888GlnIle: 1.888 ± 0.252
1.716GlnLys: 1.716 ± 0.348
3.81GlnLeu: 3.81 ± 0.414
1.27GlnMet: 1.27 ± 0.218
1.682GlnAsn: 1.682 ± 0.302
1.407GlnPro: 1.407 ± 0.197
1.682GlnGln: 1.682 ± 0.358
2.883GlnArg: 2.883 ± 0.418
1.956GlnSer: 1.956 ± 0.371
1.991GlnThr: 1.991 ± 0.302
2.265GlnVal: 2.265 ± 0.302
0.961GlnTrp: 0.961 ± 0.146
0.927GlnTyr: 0.927 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
6.281ArgAla: 6.281 ± 0.543
0.755ArgCys: 0.755 ± 0.176
4.634ArgAsp: 4.634 ± 0.383
5.114ArgGlu: 5.114 ± 0.453
2.162ArgPhe: 2.162 ± 0.254
4.771ArgGly: 4.771 ± 0.388
1.853ArgHis: 1.853 ± 0.286
3.295ArgIle: 3.295 ± 0.345
3.226ArgLys: 3.226 ± 0.32
5.148ArgLeu: 5.148 ± 0.466
1.75ArgMet: 1.75 ± 0.26
2.712ArgAsn: 2.712 ± 0.268
2.849ArgPro: 2.849 ± 0.278
2.746ArgGln: 2.746 ± 0.298
5.663ArgArg: 5.663 ± 0.469
3.913ArgSer: 3.913 ± 0.412
3.467ArgThr: 3.467 ± 0.347
5.08ArgVal: 5.08 ± 0.443
1.648ArgTrp: 1.648 ± 0.291
2.437ArgTyr: 2.437 ± 0.247
0.0ArgXaa: 0.0 ± 0.0
Ser
5.835SerAla: 5.835 ± 0.405
0.481SerCys: 0.481 ± 0.136
4.222SerAsp: 4.222 ± 0.42
3.501SerGlu: 3.501 ± 0.334
1.853SerPhe: 1.853 ± 0.191
5.08SerGly: 5.08 ± 0.532
1.27SerHis: 1.27 ± 0.246
2.712SerIle: 2.712 ± 0.315
2.197SerLys: 2.197 ± 0.283
4.119SerLeu: 4.119 ± 0.335
1.922SerMet: 1.922 ± 0.268
1.888SerAsn: 1.888 ± 0.269
2.128SerPro: 2.128 ± 0.255
1.579SerGln: 1.579 ± 0.245
3.947SerArg: 3.947 ± 0.354
3.089SerSer: 3.089 ± 0.337
3.329SerThr: 3.329 ± 0.348
4.565SerVal: 4.565 ± 0.437
1.304SerTrp: 1.304 ± 0.23
1.956SerTyr: 1.956 ± 0.259
0.0SerXaa: 0.0 ± 0.0
Thr
6.315ThrAla: 6.315 ± 0.44
0.583ThrCys: 0.583 ± 0.142
4.393ThrAsp: 4.393 ± 0.383
3.947ThrGlu: 3.947 ± 0.285
2.128ThrPhe: 2.128 ± 0.279
4.771ThrGly: 4.771 ± 0.361
1.27ThrHis: 1.27 ± 0.22
3.123ThrIle: 3.123 ± 0.362
2.609ThrLys: 2.609 ± 0.328
3.707ThrLeu: 3.707 ± 0.328
1.236ThrMet: 1.236 ± 0.206
1.853ThrAsn: 1.853 ± 0.252
4.084ThrPro: 4.084 ± 0.41
1.819ThrGln: 1.819 ± 0.296
3.638ThrArg: 3.638 ± 0.34
3.844ThrSer: 3.844 ± 0.502
3.02ThrThr: 3.02 ± 0.399
3.604ThrVal: 3.604 ± 0.321
1.064ThrTrp: 1.064 ± 0.184
1.888ThrTyr: 1.888 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
7.379ValAla: 7.379 ± 0.576
0.652ValCys: 0.652 ± 0.188
5.801ValAsp: 5.801 ± 0.394
4.805ValGlu: 4.805 ± 0.435
2.506ValPhe: 2.506 ± 0.301
5.56ValGly: 5.56 ± 0.385
1.682ValHis: 1.682 ± 0.246
3.741ValIle: 3.741 ± 0.445
2.746ValLys: 2.746 ± 0.294
4.634ValLeu: 4.634 ± 0.412
2.265ValMet: 2.265 ± 0.27
2.094ValAsn: 2.094 ± 0.294
3.055ValPro: 3.055 ± 0.386
2.197ValGln: 2.197 ± 0.211
5.251ValArg: 5.251 ± 0.375
4.29ValSer: 4.29 ± 0.434
4.599ValThr: 4.599 ± 0.46
5.56ValVal: 5.56 ± 0.52
1.339ValTrp: 1.339 ± 0.199
1.922ValTyr: 1.922 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.785TrpAla: 1.785 ± 0.238
0.309TrpCys: 0.309 ± 0.112
1.648TrpAsp: 1.648 ± 0.201
1.476TrpGlu: 1.476 ± 0.233
0.721TrpPhe: 0.721 ± 0.175
1.51TrpGly: 1.51 ± 0.242
0.789TrpHis: 0.789 ± 0.175
1.064TrpIle: 1.064 ± 0.209
0.824TrpLys: 0.824 ± 0.157
1.442TrpLeu: 1.442 ± 0.231
0.721TrpMet: 0.721 ± 0.155
0.892TrpAsn: 0.892 ± 0.179
0.892TrpPro: 0.892 ± 0.182
0.789TrpGln: 0.789 ± 0.158
1.236TrpArg: 1.236 ± 0.214
1.545TrpSer: 1.545 ± 0.318
1.579TrpThr: 1.579 ± 0.255
1.304TrpVal: 1.304 ± 0.205
0.515TrpTrp: 0.515 ± 0.121
0.549TrpTyr: 0.549 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.712TyrAla: 2.712 ± 0.327
0.24TyrCys: 0.24 ± 0.082
2.265TyrAsp: 2.265 ± 0.329
1.75TyrGlu: 1.75 ± 0.248
0.892TyrPhe: 0.892 ± 0.189
2.334TyrGly: 2.334 ± 0.237
0.652TyrHis: 0.652 ± 0.165
1.133TyrIle: 1.133 ± 0.207
0.961TyrLys: 0.961 ± 0.19
1.991TyrLeu: 1.991 ± 0.275
0.652TyrMet: 0.652 ± 0.134
1.407TyrAsn: 1.407 ± 0.241
1.03TyrPro: 1.03 ± 0.227
1.133TyrGln: 1.133 ± 0.191
2.54TyrArg: 2.54 ± 0.324
1.853TyrSer: 1.853 ± 0.329
1.545TyrThr: 1.545 ± 0.249
2.506TyrVal: 2.506 ± 0.352
0.721TyrTrp: 0.721 ± 0.17
0.652TyrTyr: 0.652 ± 0.15
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 176 proteins (29136 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski