Streptomyces phage Heather

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E3Z7|A0A4D6E3Z7_9CAUD Minor tail protein OS=Streptomyces phage Heather OX=2562343 GN=18 PE=4 SV=1
MM1 pKa = 7.94AEE3 pKa = 4.11LNDD6 pKa = 3.65WTCEE10 pKa = 3.95YY11 pKa = 10.57NGLVMGEE18 pKa = 4.12PDD20 pKa = 3.43SAVSIVAVDD29 pKa = 3.83GLLTLPEE36 pKa = 4.3IRR38 pKa = 11.84SSDD41 pKa = 3.22LSLVQRR47 pKa = 11.84HH48 pKa = 5.59GLFAGDD54 pKa = 4.48DD55 pKa = 3.82YY56 pKa = 11.69MNGRR60 pKa = 11.84TVTLTLEE67 pKa = 4.65VYY69 pKa = 10.56GSTRR73 pKa = 11.84EE74 pKa = 4.05EE75 pKa = 3.95FTQALLDD82 pKa = 3.94LQASFMPGDD91 pKa = 3.62VEE93 pKa = 3.84KK94 pKa = 10.73SLRR97 pKa = 11.84FRR99 pKa = 11.84FPGVAADD106 pKa = 3.35QTGYY110 pKa = 10.99VMCRR114 pKa = 11.84PRR116 pKa = 11.84KK117 pKa = 9.46RR118 pKa = 11.84SAPLDD123 pKa = 3.77LNFANMVCNVVVEE136 pKa = 4.99LYY138 pKa = 8.89STSPYY143 pKa = 10.27IYY145 pKa = 9.7GDD147 pKa = 3.38VARR150 pKa = 11.84TVTVQSIKK158 pKa = 10.75RR159 pKa = 11.84EE160 pKa = 4.06IVPSGLVPPSTVPWQITSQGTQPAEE185 pKa = 4.17PIARR189 pKa = 11.84FTQYY193 pKa = 10.52GSVVARR199 pKa = 11.84PTIVIQDD206 pKa = 3.47VAYY209 pKa = 7.48PTLTDD214 pKa = 3.79DD215 pKa = 3.39VTGQFFSVDD224 pKa = 3.15YY225 pKa = 11.3DD226 pKa = 3.57GDD228 pKa = 4.14LFIDD232 pKa = 3.84SAAQIVTDD240 pKa = 4.14AAGNDD245 pKa = 2.98IRR247 pKa = 11.84GLITVGSTWPEE258 pKa = 4.08FGPGAHH264 pKa = 7.23RR265 pKa = 11.84LRR267 pKa = 11.84LRR269 pKa = 11.84SRR271 pKa = 11.84DD272 pKa = 3.49EE273 pKa = 3.83YY274 pKa = 10.29TAATAVLTWSDD285 pKa = 2.93RR286 pKa = 11.84WVV288 pKa = 3.0

Molecular weight: 31.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E3Y8|A0A4D6E3Y8_9CAUD Tail terminator OS=Streptomyces phage Heather OX=2562343 GN=7 PE=4 SV=1
MM1 pKa = 7.24SRR3 pKa = 11.84SNFTVQVDD11 pKa = 3.64GLRR14 pKa = 11.84DD15 pKa = 3.6FQRR18 pKa = 11.84SVRR21 pKa = 11.84QLRR24 pKa = 11.84DD25 pKa = 2.99KK26 pKa = 10.69EE27 pKa = 4.22LNKK30 pKa = 9.98TIRR33 pKa = 11.84GANKK37 pKa = 9.31QAGEE41 pKa = 4.32LVLPAAVRR49 pKa = 11.84EE50 pKa = 4.44SPDD53 pKa = 2.82GHH55 pKa = 6.93RR56 pKa = 11.84DD57 pKa = 3.25AKK59 pKa = 10.7SSKK62 pKa = 9.9KK63 pKa = 9.86YY64 pKa = 10.53RR65 pKa = 11.84PGKK68 pKa = 9.42LDD70 pKa = 3.3KK71 pKa = 10.68SIKK74 pKa = 9.85IVASQKK80 pKa = 10.54SVAIKK85 pKa = 10.19AGSASRR91 pKa = 11.84VPYY94 pKa = 10.12AAAIHH99 pKa = 5.82FGYY102 pKa = 9.89RR103 pKa = 11.84KK104 pKa = 10.0RR105 pKa = 11.84GIRR108 pKa = 11.84PNRR111 pKa = 11.84FLYY114 pKa = 10.37RR115 pKa = 11.84AMARR119 pKa = 11.84KK120 pKa = 8.75SQAVSATYY128 pKa = 10.21EE129 pKa = 3.93RR130 pKa = 11.84LISQIVRR137 pKa = 11.84DD138 pKa = 4.1YY139 pKa = 11.56LEE141 pKa = 4.81SDD143 pKa = 3.2

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12240

50

852

214.7

23.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.018 ± 0.385

0.923 ± 0.146

6.503 ± 0.285

5.891 ± 0.395

3.088 ± 0.162

8.472 ± 0.391

1.675 ± 0.151

3.644 ± 0.223

4.632 ± 0.413

7.99 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.113

3.105 ± 0.199

5.613 ± 0.393

2.631 ± 0.132

6.732 ± 0.453

5.809 ± 0.24

7.018 ± 0.345

7.892 ± 0.214

1.732 ± 0.138

2.574 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski