Labilibacter sediminis
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4598 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9RDP8|A0A5R9RDP8_9BACT PAS domain S-box protein OS=Labilibacter sediminis OX=2570926 GN=E9993_19015 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 10.17 LIKK5 pKa = 10.45 FIDD8 pKa = 3.32 MHH10 pKa = 7.87 KK11 pKa = 10.14 IIIYY15 pKa = 9.67 GFLMLIALSACDD27 pKa = 5.08 DD28 pKa = 3.89 VNKK31 pKa = 10.36 LQLGEE36 pKa = 3.92 ITNPVLSSEE45 pKa = 4.52 VANKK49 pKa = 9.65 TIVLTDD55 pKa = 3.5 EE56 pKa = 4.61 TSDD59 pKa = 3.41 EE60 pKa = 4.34 AFDD63 pKa = 3.83 SFEE66 pKa = 3.71 WTIADD71 pKa = 4.07 FGFPSADD78 pKa = 3.35 PEE80 pKa = 4.31 YY81 pKa = 9.27 TLQMDD86 pKa = 4.08 FEE88 pKa = 6.17 GNDD91 pKa = 3.55 FQSAIALVTTFDD103 pKa = 5.1 LKK105 pKa = 11.26 LDD107 pKa = 4.45 TINALINQKK116 pKa = 10.56 LITLGAEE123 pKa = 3.78 PGVATKK129 pKa = 11.08 LEE131 pKa = 4.36 FKK133 pKa = 10.02 VTGSVHH139 pKa = 6.49 NDD141 pKa = 3.13 VIAEE145 pKa = 4.25 SNVLSATITPYY156 pKa = 10.66 EE157 pKa = 4.65 IILVYY162 pKa = 9.79 PKK164 pKa = 10.12 IYY166 pKa = 9.55 VTGDD170 pKa = 3.03 QNGWSFIEE178 pKa = 5.31 DD179 pKa = 3.5 DD180 pKa = 4.17 LLYY183 pKa = 10.8 SVSDD187 pKa = 3.57 NEE189 pKa = 3.89 IFEE192 pKa = 4.73 GYY194 pKa = 10.13 IYY196 pKa = 10.24 MGADD200 pKa = 3.14 NYY202 pKa = 11.04 FKK204 pKa = 10.38 MSYY207 pKa = 8.96 QPNWDD212 pKa = 3.6 SADD215 pKa = 4.15 AIIGDD220 pKa = 4.27 PDD222 pKa = 3.62 ASGTTGNLQVGNWGGNNIFAPDD244 pKa = 3.46 GAGVYY249 pKa = 9.48 FIKK252 pKa = 10.91 ANIPNFTYY260 pKa = 10.41 SIYY263 pKa = 8.77 KK264 pKa = 8.42 TEE266 pKa = 3.81 WAITGDD272 pKa = 3.55 FTSWGFAPMTYY283 pKa = 10.57 DD284 pKa = 3.45 EE285 pKa = 6.23 ASDD288 pKa = 3.73 TWSLTADD295 pKa = 3.38 VTAGGFKK302 pKa = 10.59 FIANEE307 pKa = 3.8 DD308 pKa = 3.22 WSKK311 pKa = 11.53 VKK313 pKa = 10.61 GDD315 pKa = 3.67 NEE317 pKa = 3.96 MDD319 pKa = 4.77 GILDD323 pKa = 4.37 KK324 pKa = 11.18 GTDD327 pKa = 3.37 EE328 pKa = 4.19 NNIVIAEE335 pKa = 4.3 DD336 pKa = 3.51 GNYY339 pKa = 9.35 TITMDD344 pKa = 4.94 LSQALYY350 pKa = 9.36 TYY352 pKa = 10.99 SVVKK356 pKa = 11.0 NNN358 pKa = 3.42
Molecular weight: 39.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.897
Patrickios 1.278
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A5R9R1D2|A0A5R9R1D2_9BACT DUF1080 domain-containing protein OS=Labilibacter sediminis OX=2570926 GN=E9993_05745 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 10.2 HH16 pKa = 3.67 GFKK19 pKa = 10.92 ARR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.01 GRR39 pKa = 11.84 KK40 pKa = 8.51 KK41 pKa = 10.08 LTVSSEE47 pKa = 3.96 KK48 pKa = 10.05 RR49 pKa = 11.84 HH50 pKa = 5.48 KK51 pKa = 10.69 AA52 pKa = 3.17
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4598
0
4598
1764307
17
6966
383.7
43.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.016 ± 0.034
0.923 ± 0.012
5.735 ± 0.028
6.604 ± 0.031
4.907 ± 0.024
6.678 ± 0.037
1.949 ± 0.017
7.779 ± 0.038
7.559 ± 0.047
8.921 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.019
6.14 ± 0.034
3.498 ± 0.02
3.349 ± 0.018
3.497 ± 0.022
6.756 ± 0.03
5.322 ± 0.04
6.452 ± 0.03
1.238 ± 0.015
4.331 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here