Luteibaculum oceani
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6V129|A0A5C6V129_9FLAO Na/Pi cotransporter family protein OS=Luteibaculum oceani OX=1294296 GN=FRX97_06655 PE=4 SV=1
MM1 pKa = 7.44 RR2 pKa = 11.84 TLTLILLLGFLPAFLIAQNNYY23 pKa = 8.35 CQPKK27 pKa = 10.15 GGSPCNFQWIEE38 pKa = 4.01 TFSCNNVLQNDD49 pKa = 5.91 GYY51 pKa = 11.04 CDD53 pKa = 4.36 RR54 pKa = 11.84 EE55 pKa = 4.57 DD56 pKa = 4.05 NDD58 pKa = 3.26 EE59 pKa = 4.16 TAPGFNPDD67 pKa = 3.55 GYY69 pKa = 11.06 SDD71 pKa = 3.84 YY72 pKa = 11.45 SDD74 pKa = 3.4 FVVIFTPGQVTTAVLTVDD92 pKa = 3.81 GNVFDD97 pKa = 5.89 EE98 pKa = 4.22 ISGWIDD104 pKa = 2.88 WDD106 pKa = 3.55 NSGTFDD112 pKa = 3.56 TDD114 pKa = 3.5 EE115 pKa = 5.05 IITFRR120 pKa = 11.84 GEE122 pKa = 4.05 STVVNEE128 pKa = 3.97 MTAVITVPSDD138 pKa = 3.14 AVQDD142 pKa = 4.03 SVILGGFRR150 pKa = 11.84 IKK152 pKa = 10.41 QDD154 pKa = 3.44 FLEE157 pKa = 5.69 PITDD161 pKa = 3.75 PCADD165 pKa = 3.44 IGNGEE170 pKa = 4.0 IEE172 pKa = 4.8 DD173 pKa = 3.7 YY174 pKa = 11.49 SFIVTNSGTTDD185 pKa = 3.15 GGANDD190 pKa = 3.7 YY191 pKa = 11.07 CSARR195 pKa = 11.84 GDD197 pKa = 4.06 SPCNFQWIEE206 pKa = 3.88 TFTCKK211 pKa = 10.39 NVIQDD216 pKa = 3.91 DD217 pKa = 5.55 GYY219 pKa = 11.54 CDD221 pKa = 5.28 RR222 pKa = 11.84 EE223 pKa = 4.59 DD224 pKa = 4.05 NDD226 pKa = 3.28 EE227 pKa = 4.16 TAPDD231 pKa = 4.6 FDD233 pKa = 5.11 PDD235 pKa = 3.98 GYY237 pKa = 11.37 SDD239 pKa = 4.05 YY240 pKa = 11.71 SDD242 pKa = 3.48 FTVIFAPGEE251 pKa = 4.32 VVTATLTVDD260 pKa = 3.41 GNVFDD265 pKa = 5.93 EE266 pKa = 4.37 ISGWLDD272 pKa = 2.83 WDD274 pKa = 5.0 NSGTWDD280 pKa = 3.6 NDD282 pKa = 3.45 EE283 pKa = 4.89 IITFRR288 pKa = 11.84 GEE290 pKa = 4.05 STVVNEE296 pKa = 3.84 MTAIITVPQDD306 pKa = 3.07 AVEE309 pKa = 4.39 GVHH312 pKa = 6.8 IIGGFRR318 pKa = 11.84 IKK320 pKa = 10.36 QDD322 pKa = 3.44 FLEE325 pKa = 5.69 PITDD329 pKa = 3.75 PCADD333 pKa = 3.44 IGNGEE338 pKa = 4.0 IEE340 pKa = 4.79 DD341 pKa = 3.71 YY342 pKa = 10.92 SFIVIPPDD350 pKa = 3.82 NIGCASSFIPQDD362 pKa = 3.32 STQNICTSTQLSWGAVSGASSYY384 pKa = 11.16 QLLVLDD390 pKa = 4.79 SSGDD394 pKa = 3.86 TIDD397 pKa = 3.87 SKK399 pKa = 11.24 QVSDD403 pKa = 3.58 TNLFVTGLRR412 pKa = 11.84 ASEE415 pKa = 4.06 AYY417 pKa = 9.18 KK418 pKa = 10.26 WIVKK422 pKa = 9.93 PIDD425 pKa = 3.57 DD426 pKa = 3.89 QGRR429 pKa = 11.84 KK430 pKa = 9.61 SFGCDD435 pKa = 2.26 TLTFFTADD443 pKa = 3.27 AADD446 pKa = 3.85 PTVSFSPDD454 pKa = 3.12 SVDD457 pKa = 3.3 VCEE460 pKa = 5.0 GRR462 pKa = 11.84 PLVLNPTITGGGTLSYY478 pKa = 10.37 NWSGDD483 pKa = 3.38 LSLLSDD489 pKa = 4.03 PAVDD493 pKa = 3.35 NPVFNSEE500 pKa = 3.84 LPEE503 pKa = 3.91 FFKK506 pKa = 11.45 YY507 pKa = 10.39 SLQVSNQYY515 pKa = 10.52 GCSNSDD521 pKa = 3.45 SLVVQVFEE529 pKa = 4.38 KK530 pKa = 10.85 GKK532 pKa = 10.37 VVNASVNSRR541 pKa = 11.84 EE542 pKa = 3.88 ICFGEE547 pKa = 4.58 DD548 pKa = 4.56 LILTLDD554 pKa = 4.38 FEE556 pKa = 6.04 ADD558 pKa = 3.22 SLNFLKK564 pKa = 10.63 QSQNGMEE571 pKa = 4.7 DD572 pKa = 3.54 VSPLNQLGDD581 pKa = 3.47 QYY583 pKa = 11.44 IFNDD587 pKa = 3.68 VDD589 pKa = 3.68 TVIYY593 pKa = 10.19 GVEE596 pKa = 4.23 LFANGCADD604 pKa = 4.02 TAWLDD609 pKa = 3.73 TVVFLPQLEE618 pKa = 4.04 IPEE621 pKa = 4.76 IIFEE625 pKa = 4.57 NPDD628 pKa = 3.41 SLGPCPGEE636 pKa = 4.45 DD637 pKa = 3.35 YY638 pKa = 11.06 LIRR641 pKa = 11.84 VNNYY645 pKa = 8.64 TDD647 pKa = 3.73 FLSWSNGASGDD658 pKa = 4.06 SIFVNEE664 pKa = 4.3 TTNLTVTYY672 pKa = 10.6 DD673 pKa = 3.79 DD674 pKa = 4.28 GCVISTDD681 pKa = 3.13 TSFTFDD687 pKa = 3.74 EE688 pKa = 4.71 VPNTILLAIDD698 pKa = 4.27 RR699 pKa = 11.84 SPALLCEE706 pKa = 4.41 GDD708 pKa = 4.18 SIEE711 pKa = 4.11 VSHH714 pKa = 7.25 GIGIDD719 pKa = 3.45 GFVWYY724 pKa = 10.09 DD725 pKa = 3.31 GDD727 pKa = 5.37 DD728 pKa = 3.65 SSLSKK733 pKa = 9.61 WVKK736 pKa = 9.46 DD737 pKa = 3.24 TTLIYY742 pKa = 10.81 VDD744 pKa = 5.36 FISDD748 pKa = 4.02 RR749 pKa = 11.84 GCVKK753 pKa = 10.56 VSDD756 pKa = 3.67 SVQFNFKK763 pKa = 10.08 PFPEE767 pKa = 4.06 QAQISSLLPLDD778 pKa = 4.24 SLCEE782 pKa = 3.96 GDD784 pKa = 3.71 VTEE787 pKa = 5.59 LVSTDD792 pKa = 3.78 SIYY795 pKa = 11.4 DD796 pKa = 3.88 LLWNTGASTPSILVSDD812 pKa = 3.94 SGKK815 pKa = 10.3 YY816 pKa = 8.16 WLEE819 pKa = 4.07 SFNGNCKK826 pKa = 9.81 TISDD830 pKa = 4.18 TLRR833 pKa = 11.84 VDD835 pKa = 4.42 FGVIPPQSSISQVVSGEE852 pKa = 4.02 DD853 pKa = 3.35 SLISFHH859 pKa = 7.15 VADD862 pKa = 4.53 HH863 pKa = 6.29 YY864 pKa = 11.73 DD865 pKa = 3.27 WYY867 pKa = 11.12 AEE869 pKa = 4.12 GQLLTFDD876 pKa = 3.67 EE877 pKa = 5.13 RR878 pKa = 11.84 IIPFVNEE885 pKa = 3.33 VTYY888 pKa = 9.66 TVQLRR893 pKa = 11.84 SSRR896 pKa = 11.84 GCLGPMSEE904 pKa = 5.03 SFFKK908 pKa = 10.93 NATSISEE915 pKa = 3.89 FQEE918 pKa = 4.02 YY919 pKa = 10.68 GFVIYY924 pKa = 10.6 KK925 pKa = 9.3 EE926 pKa = 4.04 AGRR929 pKa = 11.84 SKK931 pKa = 10.9 VKK933 pKa = 10.14 NQRR936 pKa = 11.84 PFEE939 pKa = 4.39 ASLYY943 pKa = 9.3 TIDD946 pKa = 4.32 GRR948 pKa = 11.84 LIKK951 pKa = 10.55 KK952 pKa = 10.1 LGSTFVFEE960 pKa = 5.14 WDD962 pKa = 3.35 NQGPVLLSLTLVNGKK977 pKa = 8.31 QVVLKK982 pKa = 10.77 VDD984 pKa = 3.31
Molecular weight: 108.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 1.011
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A5C6V5E2|A0A5C6V5E2_9FLAO Adenosylmethionine-8-amino-7-oxononanoate aminotransferase OS=Luteibaculum oceani OX=1294296 GN=bioA PE=3 SV=1
FF1 pKa = 7.29 KK2 pKa = 10.35 EE3 pKa = 4.01 NRR5 pKa = 11.84 GKK7 pKa = 10.33 IYY9 pKa = 10.78 AFTRR13 pKa = 11.84 LARR16 pKa = 11.84 WHH18 pKa = 6.7 EE19 pKa = 4.29 EE20 pKa = 3.85 VAQSGFKK27 pKa = 10.53 SFNTISRR34 pKa = 11.84 TIQNHH39 pKa = 4.44 YY40 pKa = 7.68 QTIINYY46 pKa = 9.29 FDD48 pKa = 3.49 NRR50 pKa = 11.84 STNAAAEE57 pKa = 4.23 SFNAKK62 pKa = 9.24 IKK64 pKa = 10.71 AFRR67 pKa = 11.84 AQFRR71 pKa = 11.84 GVRR74 pKa = 11.84 KK75 pKa = 9.71 IEE77 pKa = 3.94 FFLFRR82 pKa = 11.84 LTQIYY87 pKa = 10.32 AA88 pKa = 3.37
Molecular weight: 10.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.911
IPC_protein 10.672
Toseland 10.716
ProMoST 10.394
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.111
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.672
DTASelect 10.526
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.862
IPC_peptide 10.921
IPC2_peptide 9.326
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2382
0
2382
906267
27
4889
380.5
42.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.704 ± 0.047
1.033 ± 0.021
5.545 ± 0.045
6.716 ± 0.058
5.151 ± 0.036
7.058 ± 0.051
1.755 ± 0.024
7.211 ± 0.039
7.007 ± 0.069
9.37 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.943 ± 0.022
5.784 ± 0.047
3.94 ± 0.037
3.477 ± 0.028
3.745 ± 0.036
6.758 ± 0.044
5.381 ± 0.062
6.445 ± 0.045
1.151 ± 0.018
3.825 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here