Gordonia phage Anon
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WP92|A0A5Q2WP92_9CAUD Uncharacterized protein OS=Gordonia phage Anon OX=2653744 GN=6 PE=4 SV=1
MM1 pKa = 7.3 ATLLSILFIFFPAMVDD17 pKa = 3.6 DD18 pKa = 4.43 VPEE21 pKa = 4.51 VIADD25 pKa = 3.69 QMPVCQNEE33 pKa = 4.11 DD34 pKa = 3.63 GNPDD38 pKa = 3.59 GKK40 pKa = 10.09 PCIWYY45 pKa = 9.77 GGEE48 pKa = 4.14 HH49 pKa = 6.12 GPEE52 pKa = 3.95 GRR54 pKa = 11.84 WYY56 pKa = 10.63 VDD58 pKa = 2.92 SSVYY62 pKa = 10.22 RR63 pKa = 3.83
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.817
IPC2_protein 4.037
IPC_protein 3.91
Toseland 3.719
ProMoST 4.088
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.037
Patrickios 0.693
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A5Q2WLH9|A0A5Q2WLH9_9CAUD Scaffolding protein OS=Gordonia phage Anon OX=2653744 GN=15 PE=4 SV=1
MM1 pKa = 7.15 NWAGSTRR8 pKa = 11.84 RR9 pKa = 11.84 QRR11 pKa = 11.84 LPEE14 pKa = 4.27 DD15 pKa = 3.05 WEE17 pKa = 4.28 LNYY20 pKa = 10.43 RR21 pKa = 11.84 LPVLSRR27 pKa = 11.84 DD28 pKa = 3.11 RR29 pKa = 11.84 WLCQINGPGCTRR41 pKa = 11.84 AATDD45 pKa = 2.89 VDD47 pKa = 4.28 HH48 pKa = 7.59 IKK50 pKa = 10.86 RR51 pKa = 11.84 GDD53 pKa = 3.28 NHH55 pKa = 6.85 DD56 pKa = 3.96 RR57 pKa = 11.84 SNLQAVCFTCHH68 pKa = 5.55 NKK70 pKa = 9.83 KK71 pKa = 10.55 SSAEE75 pKa = 3.89 GVAAKK80 pKa = 10.5 AKK82 pKa = 10.43 LKK84 pKa = 9.75 AQRR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 PTEE92 pKa = 3.51 RR93 pKa = 11.84 HH94 pKa = 5.15 PGQRR98 pKa = 3.45
Molecular weight: 11.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.37
IPC_protein 9.765
Toseland 10.613
ProMoST 10.496
Dawson 10.672
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.921
Grimsley 10.701
Solomon 10.789
Lehninger 10.774
Nozaki 10.628
DTASelect 10.335
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.613
Patrickios 10.701
IPC_peptide 10.804
IPC2_peptide 9.604
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
101
0
101
16280
32
818
161.2
17.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.441 ± 0.307
0.891 ± 0.128
6.665 ± 0.189
7.058 ± 0.303
3.323 ± 0.176
8.077 ± 0.369
2.131 ± 0.148
5.104 ± 0.161
4.595 ± 0.206
7.991 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.133
3.305 ± 0.167
5.344 ± 0.245
3.421 ± 0.172
6.069 ± 0.218
5.682 ± 0.171
6.357 ± 0.221
6.935 ± 0.229
1.941 ± 0.138
3.182 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here