Magnetospirillum molischianum DSM 120

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Magnetospirillum; Magnetospirillum molischianum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8FPW2|H8FPW2_MAGML Cytochrome c biogenesis protein transmembrane region OS=Magnetospirillum molischianum DSM 120 OX=1150626 GN=PHAMO_20086 PE=4 SV=1
MM1 pKa = 7.99LSLTDD6 pKa = 3.68KK7 pKa = 10.3AVEE10 pKa = 4.44VIRR13 pKa = 11.84EE14 pKa = 4.16VCQGEE19 pKa = 4.34YY20 pKa = 9.27TGLRR24 pKa = 11.84ILVRR28 pKa = 11.84KK29 pKa = 9.13GCSGLTYY36 pKa = 11.21SMGLEE41 pKa = 3.9EE42 pKa = 5.53QAAEE46 pKa = 4.27DD47 pKa = 5.11DD48 pKa = 4.61EE49 pKa = 6.89IIDD52 pKa = 3.67IDD54 pKa = 4.01DD55 pKa = 3.51VKK57 pKa = 11.49VFVDD61 pKa = 4.0PGSALWLIGATMDD74 pKa = 3.64YY75 pKa = 10.88VADD78 pKa = 3.77STMGSGFVFDD88 pKa = 5.06NPNAVPPPPPGCSCSSGTCGG108 pKa = 4.53

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8FSJ3|H8FSJ3_MAGML Phosphoribosylglycinamide formyltransferase OS=Magnetospirillum molischianum DSM 120 OX=1150626 GN=purN PE=3 SV=1
MM1 pKa = 7.91GDD3 pKa = 3.0RR4 pKa = 11.84TQRR7 pKa = 11.84VLRR10 pKa = 11.84IAVWGLPLALLLAVILFPQIEE31 pKa = 4.21IGLTRR36 pKa = 11.84SFFDD40 pKa = 3.7AEE42 pKa = 3.72QSYY45 pKa = 9.95FPFRR49 pKa = 11.84VAPLGDD55 pKa = 4.15FVRR58 pKa = 11.84KK59 pKa = 9.45ILPIFLFALAAAVAILGVIAAIRR82 pKa = 11.84HH83 pKa = 5.08RR84 pKa = 11.84PILGVGPRR92 pKa = 11.84VALYY96 pKa = 10.92LLATLALGPGLVVNVVLKK114 pKa = 9.67DD115 pKa = 2.9HH116 pKa = 7.39WGRR119 pKa = 11.84PRR121 pKa = 11.84PSTIAEE127 pKa = 3.68FSGPNTYY134 pKa = 10.51VLPFVPSNQCDD145 pKa = 3.99DD146 pKa = 3.8NCSFPSGHH154 pKa = 6.92AALGFWTVAFALLSPPRR171 pKa = 11.84RR172 pKa = 11.84RR173 pKa = 11.84HH174 pKa = 5.54LSVASALIFGSMVGLARR191 pKa = 11.84IAQGGHH197 pKa = 5.72FLSDD201 pKa = 3.49VLVSGLLVSGLCLWLHH217 pKa = 4.26QTLIKK222 pKa = 10.59RR223 pKa = 11.84EE224 pKa = 4.2VFWPAKK230 pKa = 9.97KK231 pKa = 10.48LGSTSLL237 pKa = 3.48

Molecular weight: 25.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3771

0

3771

1146260

20

5276

304.0

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.215 ± 0.062

0.958 ± 0.015

5.825 ± 0.03

5.67 ± 0.034

3.413 ± 0.021

8.315 ± 0.037

2.075 ± 0.018

5.127 ± 0.031

2.974 ± 0.033

10.657 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.32 ± 0.022

2.422 ± 0.02

5.438 ± 0.034

3.038 ± 0.021

7.724 ± 0.039

5.634 ± 0.029

5.345 ± 0.03

7.565 ± 0.032

1.32 ± 0.018

1.96 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski