Achimota virus 2
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7XN73|K7XN73_9MONO Fusion glycoprotein F0 OS=Achimota virus 2 OX=1261101 GN=F PE=3 SV=1
MM1 pKa = 7.4 SSVFRR6 pKa = 11.84 AFEE9 pKa = 3.96 QFTLEE14 pKa = 4.13 QEE16 pKa = 4.0 QHH18 pKa = 5.41 EE19 pKa = 4.61 RR20 pKa = 11.84 GSDD23 pKa = 3.57 LEE25 pKa = 4.46 VPPEE29 pKa = 4.0 TLQTTIKK36 pKa = 10.61 VFILNTPNPQIRR48 pKa = 11.84 YY49 pKa = 9.36 QMMNFCLRR57 pKa = 11.84 IIASSSSRR65 pKa = 11.84 IAQKK69 pKa = 10.82 NGALLTLLSLPTAMMQNHH87 pKa = 5.88 IRR89 pKa = 11.84 IADD92 pKa = 4.27 RR93 pKa = 11.84 SPDD96 pKa = 3.79 SIIEE100 pKa = 4.32 RR101 pKa = 11.84 IEE103 pKa = 3.77 IEE105 pKa = 4.14 GFEE108 pKa = 3.99 QGTYY112 pKa = 10.11 RR113 pKa = 11.84 LRR115 pKa = 11.84 PNARR119 pKa = 11.84 TPMTNGEE126 pKa = 3.98 ITALEE131 pKa = 4.09 QMAEE135 pKa = 4.19 DD136 pKa = 4.16 LPEE139 pKa = 5.28 GIANDD144 pKa = 3.8 TPYY147 pKa = 10.56 INPNIEE153 pKa = 4.74 GDD155 pKa = 3.87 DD156 pKa = 3.82 CDD158 pKa = 4.54 EE159 pKa = 4.26 MEE161 pKa = 4.74 KK162 pKa = 10.54 FLNAIYY168 pKa = 10.49 SVLVQVWITVCKK180 pKa = 10.58 CMTAYY185 pKa = 9.75 DD186 pKa = 3.94 QPTGSDD192 pKa = 3.01 EE193 pKa = 4.13 RR194 pKa = 11.84 RR195 pKa = 11.84 LAKK198 pKa = 10.17 YY199 pKa = 8.02 QQQGRR204 pKa = 11.84 LDD206 pKa = 3.52 QKK208 pKa = 11.12 YY209 pKa = 9.46 VLQNEE214 pKa = 4.05 IRR216 pKa = 11.84 RR217 pKa = 11.84 IIQKK221 pKa = 9.95 CIRR224 pKa = 11.84 DD225 pKa = 3.91 SLTVRR230 pKa = 11.84 QFLTFEE236 pKa = 4.2 LQTARR241 pKa = 11.84 KK242 pKa = 8.52 QGPITSRR249 pKa = 11.84 YY250 pKa = 7.52 YY251 pKa = 11.77 AMVGDD256 pKa = 3.41 IGKK259 pKa = 9.98 YY260 pKa = 9.36 IEE262 pKa = 4.28 NAGMSAFFMTTRR274 pKa = 11.84 FALGTKK280 pKa = 8.93 WPPLALAAFSGEE292 pKa = 4.18 LIKK295 pKa = 11.03 LKK297 pKa = 10.64 SLMMLYY303 pKa = 10.14 RR304 pKa = 11.84 RR305 pKa = 11.84 LGEE308 pKa = 3.84 RR309 pKa = 11.84 ARR311 pKa = 11.84 FMALLEE317 pKa = 4.13 MQEE320 pKa = 4.06 MMEE323 pKa = 4.51 FAPANYY329 pKa = 9.1 PLTYY333 pKa = 9.81 SYY335 pKa = 11.83 AMGIGSVQDD344 pKa = 3.54 PQMRR348 pKa = 11.84 NYY350 pKa = 10.64 NFARR354 pKa = 11.84 TFLNAAYY361 pKa = 9.02 FQLGVEE367 pKa = 4.29 TANRR371 pKa = 11.84 QQGSVDD377 pKa = 2.85 KK378 pKa = 11.36 GMAEE382 pKa = 3.9 EE383 pKa = 5.05 LGLSEE388 pKa = 4.12 ADD390 pKa = 3.41 KK391 pKa = 11.39 RR392 pKa = 11.84 EE393 pKa = 3.81 MSATLTRR400 pKa = 11.84 LTTGRR405 pKa = 11.84 GAVGPAGNVDD415 pKa = 3.44 VLGRR419 pKa = 11.84 RR420 pKa = 11.84 PTTRR424 pKa = 11.84 LQQQDD429 pKa = 3.41 PAQFRR434 pKa = 11.84 VVEE437 pKa = 4.21 EE438 pKa = 4.22 DD439 pKa = 3.5 QEE441 pKa = 4.54 DD442 pKa = 5.02 DD443 pKa = 3.82 EE444 pKa = 7.26 DD445 pKa = 4.59 EE446 pKa = 5.6 DD447 pKa = 5.4 DD448 pKa = 5.37 DD449 pKa = 4.4 EE450 pKa = 7.38 GYY452 pKa = 10.04 VQPRR456 pKa = 11.84 ALQLPPNPPARR467 pKa = 11.84 NDD469 pKa = 2.63 EE470 pKa = 4.02 WFRR473 pKa = 11.84 RR474 pKa = 11.84 MAEE477 pKa = 3.81 QEE479 pKa = 4.11 VQGRR483 pKa = 11.84 LVRR486 pKa = 11.84 TTADD490 pKa = 2.52 IHH492 pKa = 6.29 QIIPATPGNQEE503 pKa = 4.24 DD504 pKa = 4.54 EE505 pKa = 4.51 NPLVDD510 pKa = 3.69 MDD512 pKa = 4.06 EE513 pKa = 4.15
Molecular weight: 58.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.881
IPC2_protein 4.876
IPC_protein 4.813
Toseland 4.673
ProMoST 4.863
Dawson 4.749
Bjellqvist 4.902
Wikipedia 4.596
Rodwell 4.647
Grimsley 4.584
Solomon 4.749
Lehninger 4.698
Nozaki 4.851
DTASelect 4.991
Thurlkill 4.66
EMBOSS 4.609
Sillero 4.914
Patrickios 4.126
IPC_peptide 4.749
IPC2_peptide 4.914
IPC2.peptide.svr19 4.891
Protein with the highest isoelectric point:
>tr|K7XBD8|K7XBD8_9MONO Phosphoprotein OS=Achimota virus 2 OX=1261101 GN=P PE=3 SV=1
MM1 pKa = 7.89 SSMASSIKK9 pKa = 10.72 LPVDD13 pKa = 3.59 LRR15 pKa = 11.84 SEE17 pKa = 4.36 KK18 pKa = 10.56 YY19 pKa = 9.48 HH20 pKa = 6.02 LHH22 pKa = 6.79 PFPIVQTPSGEE33 pKa = 4.11 DD34 pKa = 3.23 GKK36 pKa = 11.29 GSRR39 pKa = 11.84 LVKK42 pKa = 9.67 QLRR45 pKa = 11.84 IKK47 pKa = 11.05 NLMPRR52 pKa = 11.84 GSTEE56 pKa = 3.89 QPITFLNTYY65 pKa = 10.06 GFVRR69 pKa = 11.84 PMRR72 pKa = 11.84 THH74 pKa = 6.8 GEE76 pKa = 4.11 FFSEE80 pKa = 4.43 FHH82 pKa = 6.98 RR83 pKa = 11.84 PDD85 pKa = 3.09 TTPCVTACMLPFGAGPFLDD104 pKa = 3.84 NPTRR108 pKa = 11.84 LIDD111 pKa = 4.96 EE112 pKa = 4.37 IDD114 pKa = 3.42 KK115 pKa = 11.11 AHH117 pKa = 6.96 IIIRR121 pKa = 11.84 KK122 pKa = 6.96 SASYY126 pKa = 10.29 RR127 pKa = 11.84 EE128 pKa = 4.2 EE129 pKa = 4.51 IIFDD133 pKa = 3.7 IKK135 pKa = 10.66 KK136 pKa = 9.68 LPSMLTQHH144 pKa = 6.58 QLAQDD149 pKa = 4.07 KK150 pKa = 9.82 IICVSSEE157 pKa = 4.14 KK158 pKa = 10.32 FLKK161 pKa = 10.89 SPGKK165 pKa = 8.27 VTSGVDD171 pKa = 3.27 YY172 pKa = 10.51 LYY174 pKa = 10.83 SITFMSITYY183 pKa = 10.03 CPASYY188 pKa = 10.4 KK189 pKa = 10.38 FRR191 pKa = 11.84 VSRR194 pKa = 11.84 PLQIIRR200 pKa = 11.84 AKK202 pKa = 10.79 AMRR205 pKa = 11.84 SVHH208 pKa = 7.05 LEE210 pKa = 2.84 IMMRR214 pKa = 11.84 IEE216 pKa = 4.36 CKK218 pKa = 9.97 KK219 pKa = 10.72 DD220 pKa = 3.32 SPLLKK225 pKa = 10.99 NMITTDD231 pKa = 3.38 GSEE234 pKa = 4.18 GGVVSVWFHH243 pKa = 5.84 ICNLYY248 pKa = 10.31 KK249 pKa = 10.87 GNNPAKK255 pKa = 9.86 EE256 pKa = 3.94 YY257 pKa = 10.65 DD258 pKa = 3.39 DD259 pKa = 5.59 SYY261 pKa = 11.65 FSKK264 pKa = 10.41 KK265 pKa = 9.04 CKK267 pKa = 10.16 QMDD270 pKa = 3.83 IEE272 pKa = 4.52 CGIVDD277 pKa = 3.48 MWGPTLMVHH286 pKa = 6.25 AHH288 pKa = 4.22 GHH290 pKa = 5.07 IPKK293 pKa = 9.02 MAKK296 pKa = 9.95 PFFNRR301 pKa = 11.84 KK302 pKa = 7.83 GWACYY307 pKa = 9.42 PFSDD311 pKa = 5.0 AAAGLSKK318 pKa = 9.57 TLWSVGATIVEE329 pKa = 4.47 VNAILQASDD338 pKa = 3.85 LAQLTHH344 pKa = 5.61 VHH346 pKa = 6.91 DD347 pKa = 5.1 IIYY350 pKa = 9.42 PKK352 pKa = 10.77 VKK354 pKa = 10.33 LSKK357 pKa = 10.49 EE358 pKa = 3.96 LVNYY362 pKa = 9.59 QPSKK366 pKa = 10.1 WNPLKK371 pKa = 10.85 KK372 pKa = 9.85 IVSII376 pKa = 4.49
Molecular weight: 42.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.738
IPC2_protein 8.595
IPC_protein 8.463
Toseland 9.194
ProMoST 9.019
Dawson 9.487
Bjellqvist 9.311
Wikipedia 9.633
Rodwell 9.78
Grimsley 9.516
Solomon 9.531
Lehninger 9.502
Nozaki 9.502
DTASelect 9.224
Thurlkill 9.37
EMBOSS 9.663
Sillero 9.516
Patrickios 4.8
IPC_peptide 9.531
IPC2_peptide 8.009
IPC2.peptide.svr19 7.793
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5074
166
2273
634.3
71.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.011 ± 0.664
2.089 ± 0.327
5.361 ± 0.34
5.104 ± 0.48
3.587 ± 0.321
5.124 ± 0.575
2.05 ± 0.293
7.627 ± 0.599
5.282 ± 0.667
10.485 ± 1.484
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.345 ± 0.445
4.572 ± 0.495
5.518 ± 0.875
5.045 ± 0.579
4.966 ± 0.479
8.632 ± 0.695
6.445 ± 0.226
5.617 ± 0.491
1.084 ± 0.277
3.055 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here