Candidatus Accumulibacter aalborgensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A8XIM7|A0A1A8XIM7_9PROT Leucine/isoleucine/valine transporter subunit membrane component of ABC superfamily OS=Candidatus Accumulibacter aalborgensis OX=1860102 GN=livH PE=4 SV=1
MM1 pKa = 7.07ATSTKK6 pKa = 10.19FFDD9 pKa = 4.72DD10 pKa = 4.2PQSFAAVAQVLAGVIEE26 pKa = 4.22PAVPVYY32 pKa = 10.43CGSVPLGNYY41 pKa = 9.04HH42 pKa = 6.34AVEE45 pKa = 4.29VFDD48 pKa = 4.01RR49 pKa = 11.84TPNGSGYY56 pKa = 9.7PGSFVDD62 pKa = 6.23LVANTYY68 pKa = 10.82LRR70 pKa = 11.84DD71 pKa = 3.73TYY73 pKa = 11.12QKK75 pKa = 10.53PDD77 pKa = 3.37GTAGSYY83 pKa = 7.58GTSFAATFSYY93 pKa = 10.54RR94 pKa = 11.84PAAGDD99 pKa = 3.39EE100 pKa = 3.96NLAYY104 pKa = 10.68VPTVDD109 pKa = 5.22RR110 pKa = 11.84GDD112 pKa = 3.27ITIGGGARR120 pKa = 11.84YY121 pKa = 7.87TDD123 pKa = 4.19VITGHH128 pKa = 6.67FGSVANVTSTRR139 pKa = 11.84AFPDD143 pKa = 3.19PTFAFTTIGVSNVFAVQQDD162 pKa = 3.77VEE164 pKa = 4.34LATGVVRR171 pKa = 11.84TAGDD175 pKa = 3.4VLRR178 pKa = 11.84AGTLSSMFSSQQSFDD193 pKa = 3.04ASLILWEE200 pKa = 4.51DD201 pKa = 3.28RR202 pKa = 11.84AGKK205 pKa = 9.18VHH207 pKa = 7.04SLPLSDD213 pKa = 3.38ATPRR217 pKa = 11.84DD218 pKa = 3.49DD219 pKa = 5.49HH220 pKa = 8.36LFDD223 pKa = 3.55QAQEE227 pKa = 3.97LGSWVEE233 pKa = 3.95LVKK236 pKa = 10.88GAGSAWHH243 pKa = 6.99LDD245 pKa = 3.37SPTIRR250 pKa = 11.84LVIDD254 pKa = 3.85NPHH257 pKa = 6.34GLQLGLQGFLASSRR271 pKa = 11.84NPNDD275 pKa = 3.79DD276 pKa = 4.49SLNLWTEE283 pKa = 3.88VLNPSSVLAKK293 pKa = 10.33GLTYY297 pKa = 10.48RR298 pKa = 11.84VDD300 pKa = 4.13FKK302 pKa = 11.1IIAVAPLTDD311 pKa = 3.12NTTYY315 pKa = 9.16VTPFSAKK322 pKa = 10.1LAGTPFSNVTLSGTADD338 pKa = 3.25ANAIGTGGDD347 pKa = 3.37NVIEE351 pKa = 4.55GNSGANTLSGDD362 pKa = 3.76AGNDD366 pKa = 3.42TLTGSAGNDD375 pKa = 3.41TLTGGAGNDD384 pKa = 3.25SFVFADD390 pKa = 4.22HH391 pKa = 7.04GNGLDD396 pKa = 3.87TITDD400 pKa = 4.29FTPGDD405 pKa = 4.0TIDD408 pKa = 3.6VTGVTLVGHH417 pKa = 5.54VTAGDD422 pKa = 3.87GSTVGQGEE430 pKa = 4.35VQFASGTNTLYY441 pKa = 10.4IGTDD445 pKa = 3.31TTSGAEE451 pKa = 4.05VQIKK455 pKa = 10.6LIGSFTAAAFYY466 pKa = 11.19VSGSDD471 pKa = 4.36LSFGPAPTVLSFSPSDD487 pKa = 3.43GATGVATSSNIVLSFSEE504 pKa = 5.0PIAGGTGSILLQTAAGATVEE524 pKa = 4.54TYY526 pKa = 10.81DD527 pKa = 3.5AATSNRR533 pKa = 11.84LTVSGSTLTIDD544 pKa = 3.92PTSSLADD551 pKa = 3.37GTHH554 pKa = 6.3YY555 pKa = 11.0YY556 pKa = 8.6VTLAAGTIKK565 pKa = 10.8DD566 pKa = 3.93LVGNSYY572 pKa = 11.21AGTSTYY578 pKa = 11.42DD579 pKa = 3.38FTTGAAPAPSYY590 pKa = 8.53TVPGTLGNDD599 pKa = 3.55FFIPSAGNNYY609 pKa = 10.3LGGGGNDD616 pKa = 3.68TYY618 pKa = 10.81IISPNTLSGAVTAQITDD635 pKa = 3.81TEE637 pKa = 4.38GSNVIQLVDD646 pKa = 3.55GMTISASSFYY656 pKa = 11.29GNAAQLTLASGAIVQILGASALGYY680 pKa = 9.94QVGANAPAGDD690 pKa = 3.67TAATQTYY697 pKa = 9.38SQFASTLGGSVPTGSTPVSGTAGYY721 pKa = 9.48VVPSGFTQAPALTPAVAGPSYY742 pKa = 9.65TVPGTLGNDD751 pKa = 3.55FFIPSAGNNYY761 pKa = 10.3LGGGGNDD768 pKa = 3.68TYY770 pKa = 10.81IISPNTLSGAVTAQITDD787 pKa = 3.72TEE789 pKa = 4.55GVNVIQWVDD798 pKa = 3.29GMTLTASSFYY808 pKa = 11.15NDD810 pKa = 3.52AAQLTLSTGAKK821 pKa = 9.16VQILGASKK829 pKa = 10.79FNFQLGANAPAGDD842 pKa = 3.77TATSQTYY849 pKa = 10.11AQFASTLGVTLPAAGAAPVSGTPNFVVASSGFDD882 pKa = 3.26VAKK885 pKa = 10.7DD886 pKa = 3.12KK887 pKa = 11.44LVFVNTTDD895 pKa = 3.25QTVYY899 pKa = 10.69TEE901 pKa = 4.78AKK903 pKa = 9.7FKK905 pKa = 10.84ALPGVSIADD914 pKa = 3.37NPFGNNTLVYY924 pKa = 10.33LDD926 pKa = 4.42AASDD930 pKa = 3.76LSDD933 pKa = 3.21GVTLTGIADD942 pKa = 3.81APLSQIVVEE951 pKa = 4.44MMM953 pKa = 3.89

Molecular weight: 96.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A8XJ88|A0A1A8XJ88_9PROT Uncharacterized protein OS=Candidatus Accumulibacter aalborgensis OX=1860102 GN=ACCAA_180043 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 3.92GRR4 pKa = 11.84RR5 pKa = 11.84TRR7 pKa = 11.84SLEE10 pKa = 3.88RR11 pKa = 11.84TILAATSASFSSTPLRR27 pKa = 11.84EE28 pKa = 4.1CRR30 pKa = 11.84LDD32 pKa = 3.43ALGDD36 pKa = 3.77VEE38 pKa = 4.08ICKK41 pKa = 10.23PNRR44 pKa = 11.84KK45 pKa = 8.94EE46 pKa = 3.83NCCPNKK52 pKa = 9.87LHH54 pKa = 6.82VYY56 pKa = 10.01IIRR59 pKa = 11.84HH60 pKa = 5.41GLLIEE65 pKa = 4.83DD66 pKa = 4.15PSVVRR71 pKa = 11.84MHH73 pKa = 7.19RR74 pKa = 11.84AHH76 pKa = 7.46ASRR79 pKa = 11.84GQLHH83 pKa = 6.66RR84 pKa = 11.84GVSSNIRR91 pKa = 11.84TVKK94 pKa = 10.37QLLGQLARR102 pKa = 11.84NILIEE107 pKa = 4.08TLRR110 pKa = 11.84TSQRR114 pKa = 11.84LARR117 pKa = 11.84FAVCFSGLVRR127 pKa = 11.84ACAGSCKK134 pKa = 10.52NKK136 pKa = 9.73FQRR139 pKa = 11.84LQQSAVAWATAVAVAEE155 pKa = 4.8HH156 pKa = 6.03YY157 pKa = 8.98WGLVNNEE164 pKa = 3.2WRR166 pKa = 11.84VPCSRR171 pKa = 11.84VNPCGLARR179 pKa = 4.32

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4556

0

4556

1422198

19

4715

312.2

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.09 ± 0.052

1.075 ± 0.014

5.487 ± 0.027

5.54 ± 0.038

3.683 ± 0.026

7.964 ± 0.037

2.248 ± 0.022

4.765 ± 0.03

3.173 ± 0.035

11.119 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.017

2.664 ± 0.029

5.054 ± 0.025

3.769 ± 0.024

7.39 ± 0.042

5.696 ± 0.035

5.027 ± 0.041

7.341 ± 0.031

1.402 ± 0.015

2.324 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski