Gordonia phage Cozz
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160DDC4|A0A160DDC4_9CAUD Portal protein OS=Gordonia phage Cozz OX=1838066 GN=5 PE=4 SV=1
MM1 pKa = 7.29 NNEE4 pKa = 4.07 LTLSIATTHH13 pKa = 6.62 HH14 pKa = 7.01 DD15 pKa = 3.8 FVAALSLDD23 pKa = 4.31 DD24 pKa = 5.58 GDD26 pKa = 4.49 VALNCAFSSLEE37 pKa = 3.92 DD38 pKa = 3.38 MFAEE42 pKa = 4.26 VAYY45 pKa = 10.44 DD46 pKa = 3.39 VTPEE50 pKa = 3.96 LTIDD54 pKa = 3.5 YY55 pKa = 10.68 ALLFTTALVAADD67 pKa = 5.04 FIYY70 pKa = 11.01 ANYY73 pKa = 10.29
Molecular weight: 7.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.023
IPC2_protein 3.452
IPC_protein 3.363
Toseland 3.185
ProMoST 3.605
Dawson 3.401
Bjellqvist 3.579
Wikipedia 3.401
Rodwell 3.223
Grimsley 3.109
Solomon 3.338
Lehninger 3.287
Nozaki 3.554
DTASelect 3.745
Thurlkill 3.287
EMBOSS 3.401
Sillero 3.516
Patrickios 0.006
IPC_peptide 3.325
IPC2_peptide 3.465
IPC2.peptide.svr19 3.628
Protein with the highest isoelectric point:
>tr|A0A166Y4Y2|A0A166Y4Y2_9CAUD Minor tail protein OS=Gordonia phage Cozz OX=1838066 GN=17 PE=4 SV=1
MM1 pKa = 7.41 GKK3 pKa = 10.35 AGTVRR8 pKa = 11.84 LNRR11 pKa = 11.84 KK12 pKa = 7.23 GFRR15 pKa = 11.84 EE16 pKa = 3.83 IRR18 pKa = 11.84 SAPKK22 pKa = 10.46 LIRR25 pKa = 11.84 KK26 pKa = 8.55 IDD28 pKa = 4.13 KK29 pKa = 8.89 ITGGIADD36 pKa = 4.55 RR37 pKa = 11.84 ANANLDD43 pKa = 3.35 EE44 pKa = 5.31 PGYY47 pKa = 8.94 KK48 pKa = 9.53 TSSQEE53 pKa = 3.56 GAARR57 pKa = 11.84 PQGRR61 pKa = 11.84 HH62 pKa = 5.46 RR63 pKa = 11.84 GTVITATQEE72 pKa = 4.09 AKK74 pKa = 10.51 EE75 pKa = 4.23 DD76 pKa = 3.68 NANNNTLLKK85 pKa = 9.91 EE86 pKa = 4.51 FYY88 pKa = 9.94 RR89 pKa = 11.84 ARR91 pKa = 11.84 GKK93 pKa = 10.37
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 9.677
IPC_protein 10.218
Toseland 10.847
ProMoST 10.496
Dawson 10.906
Bjellqvist 10.555
Wikipedia 11.082
Rodwell 11.242
Grimsley 10.935
Solomon 11.023
Lehninger 10.994
Nozaki 10.804
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 10.994
IPC_peptide 11.023
IPC2_peptide 9.107
IPC2.peptide.svr19 8.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
14785
31
1712
217.4
24.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.976 ± 0.668
0.791 ± 0.145
6.48 ± 0.33
6.338 ± 0.465
3.172 ± 0.145
8.13 ± 0.44
2.049 ± 0.204
4.843 ± 0.203
5.188 ± 0.241
8.34 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.658 ± 0.141
3.24 ± 0.206
5.269 ± 0.248
3.666 ± 0.181
6.459 ± 0.435
5.668 ± 0.267
5.986 ± 0.264
6.892 ± 0.199
2.076 ± 0.145
2.78 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here