Cymbidium chlorotic mosaic virus
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G4DCT1|A0A0G4DCT1_9VIRU Capsid protein OS=Cymbidium chlorotic mosaic virus OX=1602124 PE=3 SV=1
MM1 pKa = 7.52 TIDD4 pKa = 5.04 IIVKK8 pKa = 10.5 NNTTPIGSKK17 pKa = 10.14 KK18 pKa = 9.85 IAVDD22 pKa = 4.19 EE23 pKa = 4.3 IFSLEE28 pKa = 3.94 VFLQGKK34 pKa = 7.96 FSWGSVATVEE44 pKa = 4.49 LTFEE48 pKa = 4.4 CRR50 pKa = 11.84 ACGYY54 pKa = 8.99 ATVRR58 pKa = 11.84 DD59 pKa = 4.01 VEE61 pKa = 4.29 FSGFLLRR68 pKa = 11.84 DD69 pKa = 3.16 RR70 pKa = 11.84 SGEE73 pKa = 3.78 YY74 pKa = 9.41 QEE76 pKa = 4.71 YY77 pKa = 10.27 IEE79 pKa = 4.35 RR80 pKa = 11.84 LVRR83 pKa = 11.84 FDD85 pKa = 4.17 SSEE88 pKa = 4.13 CQEE91 pKa = 4.25 CACRR95 pKa = 11.84 HH96 pKa = 5.61 KK97 pKa = 11.04 LCDD100 pKa = 3.49 YY101 pKa = 7.6 RR102 pKa = 11.84 TCEE105 pKa = 4.64 DD106 pKa = 4.55 PEE108 pKa = 5.31 CPDD111 pKa = 3.51 HH112 pKa = 7.51 SDD114 pKa = 3.75 KK115 pKa = 10.95 FFCEE119 pKa = 5.47 VGWCWCTNPPQTT131 pKa = 4.86
Molecular weight: 15.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.46
IPC2_protein 4.762
IPC_protein 4.635
Toseland 4.495
ProMoST 4.736
Dawson 4.584
Bjellqvist 4.724
Wikipedia 4.444
Rodwell 4.482
Grimsley 4.406
Solomon 4.571
Lehninger 4.533
Nozaki 4.685
DTASelect 4.825
Thurlkill 4.495
EMBOSS 4.457
Sillero 4.749
Patrickios 2.028
IPC_peptide 4.584
IPC2_peptide 4.749
IPC2.peptide.svr19 4.691
Protein with the highest isoelectric point:
>tr|A0A0G4DCX7|A0A0G4DCX7_9VIRU N-terminal protein OS=Cymbidium chlorotic mosaic virus OX=1602124 PE=4 SV=1
MM1 pKa = 7.95 AKK3 pKa = 9.79 RR4 pKa = 11.84 KK5 pKa = 8.95 SRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 PAKK12 pKa = 7.81 VTEE15 pKa = 4.27 ILVEE19 pKa = 4.17 APTNQSRR26 pKa = 11.84 ARR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 SSMNPSTGLRR40 pKa = 11.84 LSRR43 pKa = 11.84 PVTMPAAAGIVFGKK57 pKa = 9.87 SVPRR61 pKa = 11.84 LRR63 pKa = 11.84 SEE65 pKa = 4.24 GVSTILTNCEE75 pKa = 3.63 VNLGVVVTTGATQVVRR91 pKa = 11.84 EE92 pKa = 4.18 CAPFEE97 pKa = 4.21 VGQWLAGIAANFSKK111 pKa = 9.97 WRR113 pKa = 11.84 WLRR116 pKa = 11.84 LRR118 pKa = 11.84 FLYY121 pKa = 10.62 VPYY124 pKa = 10.54 CPTTLQGSLHH134 pKa = 6.23 MGFVYY139 pKa = 10.62 DD140 pKa = 5.81 DD141 pKa = 4.66 LDD143 pKa = 4.43 SNPTTVEE150 pKa = 3.74 AMSTLSGYY158 pKa = 6.37 TTSPLWNGAQCAPALSSVKK177 pKa = 10.45 SVVPTGSVCAVLDD190 pKa = 3.61 VTRR193 pKa = 11.84 LSKK196 pKa = 10.1 PWYY199 pKa = 9.08 PFLTAAGYY207 pKa = 9.19 TDD209 pKa = 3.55 VTEE212 pKa = 4.34 TTTALGNMYY221 pKa = 10.57 SPGKK225 pKa = 10.28 LVILTIDD232 pKa = 4.14 GSSSTAVGCGRR243 pKa = 11.84 LYY245 pKa = 10.8 AQYY248 pKa = 9.84 EE249 pKa = 4.07 IEE251 pKa = 4.91 LIEE254 pKa = 5.13 PIASSLNRR262 pKa = 3.66
Molecular weight: 28.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.917
IPC2_protein 8.931
IPC_protein 8.975
Toseland 9.253
ProMoST 9.37
Dawson 9.633
Bjellqvist 9.531
Wikipedia 9.853
Rodwell 9.721
Grimsley 9.706
Solomon 9.692
Lehninger 9.648
Nozaki 9.604
DTASelect 9.428
Thurlkill 9.487
EMBOSS 9.736
Sillero 9.663
Patrickios 4.609
IPC_peptide 9.677
IPC2_peptide 8.668
IPC2.peptide.svr19 7.878
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1878
131
940
469.5
51.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.029 ± 0.476
2.396 ± 0.525
3.94 ± 0.555
6.337 ± 0.57
2.929 ± 0.383
7.987 ± 0.366
1.278 ± 0.193
4.846 ± 0.35
4.26 ± 0.35
10.064 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.023 ± 0.12
3.994 ± 0.447
4.846 ± 0.369
2.716 ± 0.195
5.857 ± 0.311
10.011 ± 0.419
6.337 ± 1.032
8.04 ± 0.612
2.449 ± 0.284
2.662 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here