bacterium HR39

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1916 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6ATD8|A0A2H6ATD8_9BACT Superoxide dismutase OS=bacterium HR39 OX=2035434 GN=sodB PE=3 SV=1
MM1 pKa = 7.57SEE3 pKa = 4.13RR4 pKa = 11.84SVDD7 pKa = 2.99RR8 pKa = 11.84WIEE11 pKa = 3.95VATLDD16 pKa = 5.1DD17 pKa = 3.69LWEE20 pKa = 4.64GDD22 pKa = 3.84FLDD25 pKa = 4.5VEE27 pKa = 4.61VDD29 pKa = 3.55GEE31 pKa = 4.79TVLLVHH37 pKa = 6.66LPGGAIRR44 pKa = 11.84AYY46 pKa = 10.46QGICPHH52 pKa = 5.79QEE54 pKa = 3.05IALADD59 pKa = 3.59GDD61 pKa = 4.35FDD63 pKa = 4.45FEE65 pKa = 5.37SGILTCSAHH74 pKa = 6.02RR75 pKa = 11.84WQFEE79 pKa = 3.97LATGQGVNPADD90 pKa = 3.53CWLYY94 pKa = 9.38EE95 pKa = 4.1YY96 pKa = 9.89PVKK99 pKa = 11.13VEE101 pKa = 4.28DD102 pKa = 3.81DD103 pKa = 3.72KK104 pKa = 11.71IYY106 pKa = 10.45IGWVEE111 pKa = 4.13GEE113 pKa = 3.79RR114 pKa = 11.84HH115 pKa = 5.17YY116 pKa = 11.38NRR118 pKa = 11.84CAGG121 pKa = 3.5

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6AS14|A0A2H6AS14_9BACT EVE domain-containing protein OS=bacterium HR39 OX=2035434 GN=HRbin39_00084 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84PRR4 pKa = 11.84TGPSSRR10 pKa = 11.84PRR12 pKa = 11.84RR13 pKa = 11.84LLGTSRR19 pKa = 11.84LRR21 pKa = 11.84AGRR24 pKa = 11.84PASGPARR31 pKa = 11.84LPARR35 pKa = 11.84LSRR38 pKa = 11.84LRR40 pKa = 11.84PHH42 pKa = 6.17GWGRR46 pKa = 11.84TGGSPGRR53 pKa = 11.84SAPRR57 pKa = 11.84HH58 pKa = 5.08RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84LAADD65 pKa = 3.78LRR67 pKa = 11.84ARR69 pKa = 11.84GGGGPGGSRR78 pKa = 11.84GRR80 pKa = 11.84APLLHH85 pKa = 6.81RR86 pKa = 11.84ALPPARR92 pKa = 11.84RR93 pKa = 11.84GRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84GRR99 pKa = 11.84PRR101 pKa = 11.84ALHH104 pKa = 5.83RR105 pKa = 11.84LLRR108 pKa = 11.84TGALGLARR116 pKa = 11.84ARR118 pKa = 11.84RR119 pKa = 11.84PLRR122 pKa = 11.84PSPLRR127 pKa = 11.84PPAGSGGRR135 pKa = 11.84GPRR138 pKa = 11.84PVRR141 pKa = 11.84PRR143 pKa = 11.84AARR146 pKa = 11.84PAHH149 pKa = 5.9RR150 pKa = 11.84RR151 pKa = 11.84AGRR154 pKa = 11.84GHH156 pKa = 7.47AARR159 pKa = 11.84SLLRR163 pKa = 11.84PRR165 pKa = 11.84RR166 pKa = 11.84HH167 pKa = 5.31RR168 pKa = 11.84RR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84ARR173 pKa = 11.84RR174 pKa = 11.84ARR176 pKa = 11.84AGTAVGGRR184 pKa = 11.84SRR186 pKa = 11.84RR187 pKa = 11.84RR188 pKa = 11.84LLPPHH193 pKa = 6.75PGIGPGAPSRR203 pKa = 11.84RR204 pKa = 11.84TGAAGGLRR212 pKa = 11.84RR213 pKa = 11.84TEE215 pKa = 3.97RR216 pKa = 11.84PSLPRR221 pKa = 11.84HH222 pKa = 5.7RR223 pKa = 11.84PRR225 pKa = 11.84SRR227 pKa = 11.84GDD229 pKa = 2.96GRR231 pKa = 11.84ALARR235 pKa = 11.84GGLAVLHH242 pKa = 6.58PRR244 pKa = 11.84LAHH247 pKa = 5.76GPSRR251 pKa = 11.84PRR253 pKa = 11.84PRR255 pKa = 11.84AAAPQPLLRR264 pKa = 11.84LLPHH268 pKa = 6.72PRR270 pKa = 11.84RR271 pKa = 11.84GAWGRR276 pKa = 11.84GSARR280 pKa = 11.84SDD282 pKa = 2.91GCAAHH287 pKa = 7.12AGTFHH292 pKa = 7.56RR293 pKa = 11.84RR294 pKa = 11.84RR295 pKa = 11.84PPLLAARR302 pKa = 11.84RR303 pKa = 11.84ALLRR307 pKa = 11.84RR308 pKa = 11.84CSGPLPRR315 pKa = 11.84GRR317 pKa = 11.84TAVAPAGGGAGHH329 pKa = 7.08RR330 pKa = 11.84RR331 pKa = 11.84RR332 pKa = 11.84HH333 pKa = 5.36PRR335 pKa = 11.84APPRR339 pKa = 11.84RR340 pKa = 11.84RR341 pKa = 11.84LLGLGQAGRR350 pKa = 11.84IHH352 pKa = 6.43GRR354 pKa = 11.84PHH356 pKa = 6.05EE357 pKa = 4.29ARR359 pKa = 11.84GADD362 pKa = 3.5VGAGAARR369 pKa = 11.84GDD371 pKa = 3.43GHH373 pKa = 6.77RR374 pKa = 11.84RR375 pKa = 11.84GPRR378 pKa = 11.84GTTASGGGAPLLLPCAAAVRR398 pKa = 11.84TGEE401 pKa = 4.09GMPP404 pKa = 3.92

Molecular weight: 43.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1916

0

1916

551194

29

1434

287.7

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.234 ± 0.081

0.878 ± 0.02

5.048 ± 0.045

7.316 ± 0.062

3.302 ± 0.042

9.18 ± 0.062

2.337 ± 0.037

3.473 ± 0.046

1.648 ± 0.029

11.462 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.027

1.392 ± 0.025

6.577 ± 0.049

2.234 ± 0.036

10.647 ± 0.076

3.598 ± 0.038

4.096 ± 0.034

8.676 ± 0.055

1.388 ± 0.025

1.738 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski