Acinetobacter phage Henu6
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410T5J9|A0A410T5J9_9CAUD Uncharacterized protein OS=Acinetobacter phage Henu6 OX=2500136 GN=Henu6_gp205 PE=4 SV=1
MM1 pKa = 7.37 RR2 pKa = 11.84 HH3 pKa = 5.09 MMEE6 pKa = 4.85 NLIQPTEE13 pKa = 3.95 EE14 pKa = 3.74 QFEE17 pKa = 4.42 QIRR20 pKa = 11.84 DD21 pKa = 3.81 EE22 pKa = 5.7 LEE24 pKa = 4.19 GCSDD28 pKa = 4.16 DD29 pKa = 4.49 FGRR32 pKa = 11.84 THH34 pKa = 6.82 KK35 pKa = 10.59 RR36 pKa = 11.84 VLTEE40 pKa = 4.72 LGLDD44 pKa = 3.25 TDD46 pKa = 4.54 LRR48 pKa = 11.84 DD49 pKa = 3.59 SVIYY53 pKa = 10.8 NDD55 pKa = 3.66 TLEE58 pKa = 4.63 TYY60 pKa = 9.95 TFLCEE65 pKa = 6.07 NCDD68 pKa = 3.79 CWCSPDD74 pKa = 3.58 TRR76 pKa = 11.84 VHH78 pKa = 5.2 NQIAEE83 pKa = 4.09 MTVCEE88 pKa = 4.34 EE89 pKa = 4.57 CDD91 pKa = 3.44 EE92 pKa = 4.19 QLSS95 pKa = 3.76
Molecular weight: 11.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 4.126
IPC_protein 4.05
Toseland 3.872
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.884
Grimsley 3.783
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.164
Patrickios 1.036
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.043
Protein with the highest isoelectric point:
>tr|A0A410T5N8|A0A410T5N8_9CAUD Uncharacterized protein OS=Acinetobacter phage Henu6 OX=2500136 GN=Henu6_gp25 PE=4 SV=1
MM1 pKa = 7.96 RR2 pKa = 11.84 VLQNQLKK9 pKa = 8.78 EE10 pKa = 4.07 VSFFTSCINIKK21 pKa = 9.03 KK22 pKa = 7.78 TFNVFLNTVQFIIIGTVSNTYY43 pKa = 10.29 RR44 pKa = 11.84 CNTWPRR50 pKa = 11.84 PHH52 pKa = 6.72 GFHH55 pKa = 5.72 YY56 pKa = 9.65 TFNISASRR64 pKa = 11.84 NNKK67 pKa = 9.39 LCLL70 pKa = 3.51
Molecular weight: 8.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.458
IPC_protein 9.604
Toseland 10.175
ProMoST 9.838
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.511
Rodwell 10.804
Grimsley 10.409
Solomon 10.409
Lehninger 10.379
Nozaki 10.277
DTASelect 10.014
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.657
IPC_peptide 10.409
IPC2_peptide 9.268
IPC2.peptide.svr19 8.242
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
234
0
234
51348
68
1385
219.4
24.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.023 ± 0.205
1.071 ± 0.064
6.168 ± 0.126
6.429 ± 0.211
4.281 ± 0.123
5.809 ± 0.238
1.914 ± 0.088
7.375 ± 0.143
7.383 ± 0.189
8.02 ± 0.142
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.701 ± 0.096
5.93 ± 0.133
3.628 ± 0.097
3.554 ± 0.113
4.172 ± 0.107
6.251 ± 0.144
6.364 ± 0.231
6.477 ± 0.123
1.34 ± 0.055
4.101 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here