Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus thermophilus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1577 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5M5M7|Y441_STRT2 UPF0346 protein stu0441 OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) OX=264199 GN=stu0441 PE=3 SV=1
MM1 pKa = 7.52LSKK4 pKa = 10.94SKK6 pKa = 7.14TTKK9 pKa = 10.54ALLYY13 pKa = 9.35STAALSLFAASHH25 pKa = 4.36VHH27 pKa = 7.09ADD29 pKa = 3.94EE30 pKa = 4.49TSHH33 pKa = 4.16WTARR37 pKa = 11.84SVDD40 pKa = 3.79QIKK43 pKa = 11.06ADD45 pKa = 3.1ISVNDD50 pKa = 3.47NQQTYY55 pKa = 7.44TVQYY59 pKa = 10.1GDD61 pKa = 3.61TLGSIVEE68 pKa = 4.11AMGIDD73 pKa = 3.8MNVLANIKK81 pKa = 10.03EE82 pKa = 4.0ITNIDD87 pKa = 4.33LIFPGTVLTTTYY99 pKa = 10.87NADD102 pKa = 3.45NQAVSVKK109 pKa = 10.39VEE111 pKa = 4.34TPSSEE116 pKa = 4.19TSDD119 pKa = 3.54TPVVAEE125 pKa = 4.68SNLTTNEE132 pKa = 3.73VTVNGQSVVASDD144 pKa = 3.82LSAPVEE150 pKa = 4.56TVSLTATQAPAKK162 pKa = 9.83EE163 pKa = 4.44EE164 pKa = 4.06STQVVSEE171 pKa = 4.18VTEE174 pKa = 5.01AIASASDD181 pKa = 3.26TPAYY185 pKa = 10.45ADD187 pKa = 3.6TEE189 pKa = 4.45QPVADD194 pKa = 5.58AIDD197 pKa = 4.14HH198 pKa = 5.3VTSSAEE204 pKa = 3.61EE205 pKa = 3.83TLAEE209 pKa = 4.14EE210 pKa = 4.22EE211 pKa = 4.45APATEE216 pKa = 4.4TSAQAEE222 pKa = 4.48TTEE225 pKa = 4.18VAATSEE231 pKa = 4.33AASEE235 pKa = 4.17AASEE239 pKa = 4.58VPAEE243 pKa = 4.0QLAAASEE250 pKa = 4.33APEE253 pKa = 4.25SSEE256 pKa = 5.06APAEE260 pKa = 4.16QPAAAPEE267 pKa = 4.32SSEE270 pKa = 4.72APAEE274 pKa = 4.18QPAATSEE281 pKa = 4.22AASEE285 pKa = 4.42APASVVPVATSEE297 pKa = 4.29AVSEE301 pKa = 4.27APAVSEE307 pKa = 4.69VPAEE311 pKa = 3.97QLAAASEE318 pKa = 4.35APEE321 pKa = 4.19SSEE324 pKa = 4.69VPAEE328 pKa = 4.13QPAAAPEE335 pKa = 4.32SSEE338 pKa = 4.72APAEE342 pKa = 4.18QPAATSEE349 pKa = 4.11AAPATSEE356 pKa = 4.35APAEE360 pKa = 3.94QLAATSEE367 pKa = 4.25AASTPNTYY375 pKa = 10.0PVGQCTWGAKK385 pKa = 9.56SLAPWAGNNWGNAKK399 pKa = 10.27DD400 pKa = 4.0WIASAQAAGHH410 pKa = 5.73SVGTTPVAGAIAVWPNDD427 pKa = 3.19GGGYY431 pKa = 9.63GHH433 pKa = 6.72VAYY436 pKa = 8.98VTSASGVNSIQVMEE450 pKa = 4.5SNYY453 pKa = 10.27AGNMLIGNYY462 pKa = 9.71RR463 pKa = 11.84GTFDD467 pKa = 3.96PTSSAHH473 pKa = 6.21GGSVYY478 pKa = 10.67YY479 pKa = 10.14IYY481 pKa = 10.65PP482 pKa = 3.8

Molecular weight: 48.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5M2L3|Q5M2L3_STRT2 CoA_binding domain-containing protein OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) OX=264199 GN=stu1802 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.67IRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.52HH16 pKa = 5.2GFRR19 pKa = 11.84HH20 pKa = 6.39RR21 pKa = 11.84MSTKK25 pKa = 9.02NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 8.75VLAAA44 pKa = 4.31

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1577

0

1577

458119

31

1470

290.5

32.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.484 ± 0.073

0.563 ± 0.016

5.87 ± 0.056

6.868 ± 0.068

4.607 ± 0.042

6.597 ± 0.058

1.893 ± 0.027

7.431 ± 0.059

6.879 ± 0.066

9.965 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.03

4.59 ± 0.04

3.251 ± 0.033

3.732 ± 0.043

4.12 ± 0.048

6.239 ± 0.061

5.67 ± 0.047

7.15 ± 0.054

0.831 ± 0.02

3.723 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski