Gordonia phage Bantam

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bantamvirus; Gordonia virus Bantam

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3AYN6|A0A1B3AYN6_9CAUD Uncharacterized protein OS=Gordonia phage Bantam OX=1887641 GN=168 PE=4 SV=1
MM1 pKa = 7.91HH2 pKa = 7.86DD3 pKa = 4.18NDD5 pKa = 4.55IDD7 pKa = 4.38DD8 pKa = 3.99EE9 pKa = 4.53TTEE12 pKa = 3.83VEE14 pKa = 4.18LRR16 pKa = 11.84HH17 pKa = 5.42TVTLQVEE24 pKa = 4.52LTVEE28 pKa = 4.88GYY30 pKa = 10.8YY31 pKa = 10.1PDD33 pKa = 5.4DD34 pKa = 5.12DD35 pKa = 4.87YY36 pKa = 12.19EE37 pKa = 4.83GACSMAAQRR46 pKa = 11.84AQEE49 pKa = 3.98ALCLRR54 pKa = 11.84FAPGTHH60 pKa = 6.68IDD62 pKa = 4.97DD63 pKa = 3.52ITTIDD68 pKa = 3.27SRR70 pKa = 11.84EE71 pKa = 3.79II72 pKa = 3.47

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3AY69|A0A1B3AY69_9CAUD Uncharacterized protein OS=Gordonia phage Bantam OX=1887641 GN=5 PE=4 SV=1
MM1 pKa = 7.39AVSLIKK7 pKa = 10.3RR8 pKa = 11.84PKK10 pKa = 8.62VSLRR14 pKa = 11.84KK15 pKa = 8.46PVNMRR20 pKa = 11.84KK21 pKa = 9.18VAMVRR26 pKa = 11.84GG27 pKa = 3.84

Molecular weight: 3.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

0

168

28856

27

2944

171.8

18.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.809 ± 0.476

0.856 ± 0.123

7.298 ± 0.19

6.921 ± 0.265

3.088 ± 0.146

8.123 ± 0.261

2.135 ± 0.161

5.302 ± 0.144

3.746 ± 0.188

7.804 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.088

3.181 ± 0.147

4.897 ± 0.179

3.244 ± 0.24

7.17 ± 0.192

5.489 ± 0.185

5.978 ± 0.17

6.824 ± 0.259

1.986 ± 0.132

2.724 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski