Gordonia phage GMA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A159B6L5|A0A159B6L5_9CAUD Uncharacterized protein OS=Gordonia phage GMA1 OX=1647470 GN=GMA1_54 PE=4 SV=1
MM1 pKa = 7.64TGTDD5 pKa = 3.65PAVEE9 pKa = 4.16AARR12 pKa = 11.84LAAASGWDD20 pKa = 3.3VLYY23 pKa = 11.19CEE25 pKa = 5.7FSQEE29 pKa = 4.23DD30 pKa = 4.53LVTAARR36 pKa = 11.84EE37 pKa = 3.73ALAPIRR43 pKa = 11.84EE44 pKa = 4.28LHH46 pKa = 6.32RR47 pKa = 11.84GEE49 pKa = 5.35PYY51 pKa = 10.8AQGPDD56 pKa = 3.29YY57 pKa = 11.32CEE59 pKa = 4.2EE60 pKa = 5.12CEE62 pKa = 4.42HH63 pKa = 6.25QWPCPTARR71 pKa = 11.84LIYY74 pKa = 10.56SEE76 pKa = 4.72TEE78 pKa = 3.95LTNDD82 pKa = 3.13

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A159B6K8|A0A159B6K8_9CAUD Uncharacterized protein OS=Gordonia phage GMA1 OX=1647470 GN=GMA1_48 PE=4 SV=1
MM1 pKa = 7.56SNDD4 pKa = 3.43RR5 pKa = 11.84EE6 pKa = 4.31SWRR9 pKa = 11.84PIPGWEE15 pKa = 3.91NFYY18 pKa = 10.88EE19 pKa = 4.31VSDD22 pKa = 3.98LGRR25 pKa = 11.84VRR27 pKa = 11.84SVNRR31 pKa = 11.84TIEE34 pKa = 3.9TRR36 pKa = 11.84PGVFANRR43 pKa = 11.84RR44 pKa = 11.84GRR46 pKa = 11.84VLTPSLSPDD55 pKa = 3.27GYY57 pKa = 10.72AHH59 pKa = 6.73VWLCRR64 pKa = 11.84GNRR67 pKa = 11.84RR68 pKa = 11.84TFQRR72 pKa = 11.84VSRR75 pKa = 11.84AVATAWHH82 pKa = 6.58GPCPKK87 pKa = 10.49GMEE90 pKa = 4.28CRR92 pKa = 11.84HH93 pKa = 6.31LDD95 pKa = 3.38GDD97 pKa = 4.0KK98 pKa = 11.19TNNTPANLAWGSRR111 pKa = 11.84SEE113 pKa = 4.08NTHH116 pKa = 6.36DD117 pKa = 3.68KK118 pKa = 10.33VRR120 pKa = 11.84HH121 pKa = 5.02GTHH124 pKa = 5.49PMARR128 pKa = 11.84KK129 pKa = 6.08THH131 pKa = 6.36CKK133 pKa = 9.73RR134 pKa = 11.84GHH136 pKa = 6.57PLSGPNVYY144 pKa = 9.56MINGGRR150 pKa = 11.84RR151 pKa = 11.84CRR153 pKa = 11.84TCVNQQKK160 pKa = 9.82RR161 pKa = 11.84DD162 pKa = 3.06RR163 pKa = 11.84RR164 pKa = 11.84ARR166 pKa = 11.84IKK168 pKa = 10.66HH169 pKa = 4.74EE170 pKa = 4.19RR171 pKa = 11.84KK172 pKa = 9.67VAA174 pKa = 3.4

Molecular weight: 20.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13085

37

1641

192.4

20.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.572 ± 0.759

1.223 ± 0.24

6.878 ± 0.277

5.64 ± 0.287

2.216 ± 0.167

8.59 ± 0.406

2.682 ± 0.28

4.608 ± 0.214

3.37 ± 0.306

7.459 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.135

2.629 ± 0.233

5.25 ± 0.284

3.317 ± 0.218

7.253 ± 0.436

5.632 ± 0.324

7.276 ± 0.239

7.077 ± 0.353

2.102 ± 0.169

2.025 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski