Streptomyces sp. ADI96-02

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6EBI1|A0A3N6EBI1_9ACTN Uncharacterized protein OS=Streptomyces sp. ADI96-02 OX=1522760 GN=EES43_24755 PE=4 SV=1
MM1 pKa = 7.73SNPQQLRR8 pKa = 11.84YY9 pKa = 10.06SKK11 pKa = 9.49EE12 pKa = 4.2HH13 pKa = 5.77EE14 pKa = 4.11WLSAVEE20 pKa = 4.84DD21 pKa = 4.4GVATVGITEE30 pKa = 4.15YY31 pKa = 11.0AANALGDD38 pKa = 3.65VVYY41 pKa = 10.63AQLPEE46 pKa = 4.03VGATVTAGEE55 pKa = 4.45TCGEE59 pKa = 4.13LEE61 pKa = 4.31STKK64 pKa = 10.61SVSDD68 pKa = 4.05LYY70 pKa = 11.58SPVTGEE76 pKa = 3.73VTAANQDD83 pKa = 3.78VVDD86 pKa = 5.35DD87 pKa = 4.3PSLVNTAPFEE97 pKa = 4.49GGWLFKK103 pKa = 11.04VRR105 pKa = 11.84VAQEE109 pKa = 3.96PDD111 pKa = 3.43DD112 pKa = 4.84LLSADD117 pKa = 4.59EE118 pKa = 4.11YY119 pKa = 11.13TAFSGNN125 pKa = 3.27

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6HN19|A0A3N6HN19_9ACTN Uncharacterized protein OS=Streptomyces sp. ADI96-02 OX=1522760 GN=EES43_11880 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.57GRR40 pKa = 11.84SSLSAA45 pKa = 3.62

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5946

0

5946

1954771

29

6772

328.8

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.972 ± 0.048

0.789 ± 0.009

6.027 ± 0.021

5.742 ± 0.032

2.672 ± 0.017

9.644 ± 0.036

2.223 ± 0.015

3.071 ± 0.021

2.068 ± 0.032

10.159 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.701 ± 0.015

1.706 ± 0.018

6.28 ± 0.033

2.641 ± 0.02

8.367 ± 0.042

5.153 ± 0.023

5.96 ± 0.029

8.359 ± 0.028

1.451 ± 0.014

2.015 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski