Hyphomonas johnsonii MHS-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Hyphomonas; Hyphomonas johnsonii

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059FEC8|A0A059FEC8_9PROT Carboxyl transferase domain-containing protein OS=Hyphomonas johnsonii MHS-2 OX=1280950 GN=HJO_15189 PE=4 SV=1
MM1 pKa = 7.48NGNTIKK7 pKa = 10.73SRR9 pKa = 11.84LLASSVFAGVAFMASASMLATAQEE33 pKa = 4.45AEE35 pKa = 4.37DD36 pKa = 3.84TVDD39 pKa = 3.4TVEE42 pKa = 4.9APAAEE47 pKa = 4.64DD48 pKa = 3.14ATARR52 pKa = 11.84QATVYY57 pKa = 9.11VTGSRR62 pKa = 11.84IASPNLVTTSPVSTVTAADD81 pKa = 3.34IKK83 pKa = 10.99LQGATKK89 pKa = 10.5VEE91 pKa = 4.11DD92 pKa = 5.65LITQLPQAFAAQNSTVSNGASGTATVSLRR121 pKa = 11.84NLDD124 pKa = 3.81LGGSATRR131 pKa = 11.84TLVLIDD137 pKa = 4.35GKK139 pKa = 10.77RR140 pKa = 11.84MPYY143 pKa = 10.38GSPNDD148 pKa = 3.62AAADD152 pKa = 3.89LNLIPGALVEE162 pKa = 4.44RR163 pKa = 11.84VDD165 pKa = 3.85VLTGGASAVYY175 pKa = 10.33GSDD178 pKa = 2.82AVAGVVNFIMKK189 pKa = 10.55DD190 pKa = 3.16NFEE193 pKa = 4.27GVQLDD198 pKa = 3.57AQYY201 pKa = 11.75GFFQHH206 pKa = 6.53NNDD209 pKa = 3.21YY210 pKa = 8.77DD211 pKa = 4.05TNGNLRR217 pKa = 11.84SVIADD222 pKa = 4.23RR223 pKa = 11.84AATNPSQFQLPDD235 pKa = 3.77DD236 pKa = 4.15NVIDD240 pKa = 4.89GYY242 pKa = 10.9SKK244 pKa = 10.84EE245 pKa = 3.69ITAIMGVNTDD255 pKa = 4.01DD256 pKa = 4.52GRR258 pKa = 11.84GNLTGYY264 pKa = 10.37ISFRR268 pKa = 11.84DD269 pKa = 3.49NDD271 pKa = 3.6EE272 pKa = 4.07VLGGDD277 pKa = 3.88RR278 pKa = 11.84DD279 pKa = 3.94YY280 pKa = 11.39SACAIGSATATGFTCGGSATSVPGYY305 pKa = 10.59FLTQNNDD312 pKa = 2.69EE313 pKa = 4.44FTLDD317 pKa = 3.45SATGNTFRR325 pKa = 11.84PFDD328 pKa = 4.4SDD330 pKa = 3.26SDD332 pKa = 4.18LYY334 pKa = 11.75NFGPLNFYY342 pKa = 10.23QRR344 pKa = 11.84PDD346 pKa = 2.95QRR348 pKa = 11.84YY349 pKa = 7.9SVGFMGHH356 pKa = 5.95YY357 pKa = 9.27EE358 pKa = 4.02LNPNVEE364 pKa = 4.73AYY366 pKa = 7.74TQFMFMDD373 pKa = 4.07YY374 pKa = 10.8EE375 pKa = 4.32SRR377 pKa = 11.84AQIAPTGNFFSTNSINCDD395 pKa = 3.2NPLLSGSQVTDD406 pKa = 4.17LGCTPANVANGDD418 pKa = 4.01SVTMYY423 pKa = 9.32VARR426 pKa = 11.84RR427 pKa = 11.84NVEE430 pKa = 3.7GGGRR434 pKa = 11.84TDD436 pKa = 3.19NLGYY440 pKa = 8.05QTYY443 pKa = 9.97RR444 pKa = 11.84GVVGLRR450 pKa = 11.84GDD452 pKa = 3.97LFDD455 pKa = 4.49AAGWTYY461 pKa = 11.2DD462 pKa = 3.15VSAQYY467 pKa = 10.95SQVTLSRR474 pKa = 11.84AYY476 pKa = 8.96TNEE479 pKa = 3.49FSVTRR484 pKa = 11.84LNRR487 pKa = 11.84ALNVVDD493 pKa = 5.71DD494 pKa = 4.33GTGTPVCASVLDD506 pKa = 4.27GTDD509 pKa = 3.78PNCVPWDD516 pKa = 3.1IFTIGNVSQASLDD529 pKa = 3.88YY530 pKa = 11.01LQVPLLQNGTTTQNIVSAVVSGDD553 pKa = 2.98LGQYY557 pKa = 8.96GFKK560 pKa = 10.73SPAADD565 pKa = 2.93EE566 pKa = 4.44GMKK569 pKa = 10.15VAAGVEE575 pKa = 4.01YY576 pKa = 10.62RR577 pKa = 11.84RR578 pKa = 11.84DD579 pKa = 3.57SLEE582 pKa = 4.05SVTDD586 pKa = 3.52NSFATGDD593 pKa = 3.66GAGQGGPTIGLSGAIDD609 pKa = 3.5SYY611 pKa = 11.11EE612 pKa = 4.02AFGEE616 pKa = 4.21FQMPLVTGQPGVEE629 pKa = 3.98LLSIEE634 pKa = 4.03GAYY637 pKa = 9.94RR638 pKa = 11.84YY639 pKa = 10.03SDD641 pKa = 3.75YY642 pKa = 10.97STGSSSEE649 pKa = 4.08AYY651 pKa = 9.77KK652 pKa = 10.94LGGDD656 pKa = 3.77YY657 pKa = 11.18APTQDD662 pKa = 2.45IRR664 pKa = 11.84FRR666 pKa = 11.84ASYY669 pKa = 8.61QQAVRR674 pKa = 11.84APNVIDD680 pKa = 4.84LFQAQGFNLFDD691 pKa = 6.12LDD693 pKa = 6.14DD694 pKa = 4.94DD695 pKa = 4.9LCDD698 pKa = 3.95FTDD701 pKa = 4.31PEE703 pKa = 4.28NDD705 pKa = 3.53GTGGAACIGSNPWQVTQAQADD726 pKa = 3.96GGLLDD731 pKa = 5.0SPAGQYY737 pKa = 11.03NFLQGGNPNLEE748 pKa = 4.17PEE750 pKa = 4.17EE751 pKa = 4.61AKK753 pKa = 10.13TFTIGFVATPTFIPGLSLSLDD774 pKa = 3.78YY775 pKa = 11.5YY776 pKa = 11.24DD777 pKa = 5.42IDD779 pKa = 3.52IEE781 pKa = 4.33KK782 pKa = 10.68AISTVGASVTMQLCYY797 pKa = 10.85LEE799 pKa = 5.66GDD801 pKa = 3.49ADD803 pKa = 3.66ACSRR807 pKa = 11.84INRR810 pKa = 11.84NANGQLWVGTGNVVDD825 pKa = 3.88TNINIGGVSTSGYY838 pKa = 10.07DD839 pKa = 3.11VSAAYY844 pKa = 10.11GFDD847 pKa = 3.31AGTAGSFNLSMNGTYY862 pKa = 10.37LDD864 pKa = 4.34SFDD867 pKa = 4.4VDD869 pKa = 4.38PIGQSFAEE877 pKa = 4.11YY878 pKa = 10.47DD879 pKa = 3.79CVGEE883 pKa = 4.02YY884 pKa = 11.23GNDD887 pKa = 3.48CFTPTPEE894 pKa = 3.49WRR896 pKa = 11.84HH897 pKa = 5.15RR898 pKa = 11.84ARR900 pKa = 11.84LSWSTPVDD908 pKa = 3.49ALEE911 pKa = 5.46LNATWRR917 pKa = 11.84YY918 pKa = 9.15IGEE921 pKa = 4.12VDD923 pKa = 5.15LDD925 pKa = 3.67TGATGRR931 pKa = 11.84VDD933 pKa = 3.45STLDD937 pKa = 3.18AQNYY941 pKa = 8.89FDD943 pKa = 6.4LAGTWGATDD952 pKa = 3.41YY953 pKa = 10.39ATFRR957 pKa = 11.84FGVNNVLDD965 pKa = 4.89DD966 pKa = 5.01DD967 pKa = 5.07PPLSASVGTTGNNNTYY983 pKa = 8.6PQSYY987 pKa = 9.45DD988 pKa = 2.97ALGRR992 pKa = 11.84YY993 pKa = 8.95IFVGATLDD1001 pKa = 3.63FF1002 pKa = 4.87

Molecular weight: 106.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059FNV0|A0A059FNV0_9PROT Pyruvate dehydrogenase E1 component subunit beta OS=Hyphomonas johnsonii MHS-2 OX=1280950 GN=HJO_08839 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.04RR14 pKa = 11.84THH16 pKa = 5.87GFRR19 pKa = 11.84LRR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.16EE41 pKa = 3.63LSAA44 pKa = 4.99

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3447

0

3447

1092738

40

2749

317.0

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.64 ± 0.062

0.848 ± 0.012

6.3 ± 0.032

5.711 ± 0.036

3.795 ± 0.025

8.628 ± 0.04

1.933 ± 0.02

5.121 ± 0.03

3.38 ± 0.035

9.655 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.021

2.684 ± 0.027

5.147 ± 0.026

2.986 ± 0.02

6.57 ± 0.04

5.55 ± 0.03

5.537 ± 0.023

7.22 ± 0.029

1.383 ± 0.016

2.301 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski