Rothia kristinae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Rothia

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1872 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A199NUK7|A0A199NUK7_9MICC Thiol-disulfide isomerase OS=Rothia kristinae OX=37923 GN=AN277_0203740 PE=4 SV=1
MM1 pKa = 7.77ADD3 pKa = 3.44YY4 pKa = 11.36EE5 pKa = 4.34EE6 pKa = 4.89FDD8 pKa = 3.88GDD10 pKa = 3.68GEE12 pKa = 4.35EE13 pKa = 4.63YY14 pKa = 10.37EE15 pKa = 4.57RR16 pKa = 11.84EE17 pKa = 3.99APEE20 pKa = 3.97VYY22 pKa = 9.59RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84IIAAIIAILAIILVVWALVFAFQRR50 pKa = 11.84LTDD53 pKa = 4.02SGDD56 pKa = 3.36DD57 pKa = 3.34TAAASSAAAGDD68 pKa = 3.93NFDD71 pKa = 4.45SFSARR76 pKa = 11.84PTGGASGSASGSASAQPSASASAEE100 pKa = 3.77ATPTGEE106 pKa = 4.29STASADD112 pKa = 4.15AEE114 pKa = 4.34GTEE117 pKa = 4.77ASATPTDD124 pKa = 3.78QAAAPTPEE132 pKa = 4.58PTTQQPVQACSDD144 pKa = 3.62AFTVSTTVDD153 pKa = 3.33KK154 pKa = 10.96QVYY157 pKa = 9.52AAGQQPVITTTVANGSQNPCTVDD180 pKa = 3.23LGSANTTYY188 pKa = 10.77QITSGPANVYY198 pKa = 10.43SSQTCQAQPTHH209 pKa = 7.51DD210 pKa = 4.2EE211 pKa = 4.07ATLQAGAKK219 pKa = 7.42QTTSLTWDD227 pKa = 3.18RR228 pKa = 11.84GMNAYY233 pKa = 10.18GCGQAAQQAKK243 pKa = 8.35TGYY246 pKa = 8.57YY247 pKa = 9.04WVTATVNGVSSQPTLIVIQQ266 pKa = 3.9

Molecular weight: 27.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A199NTR6|A0A199NTR6_9MICC Uncharacterized protein OS=Rothia kristinae OX=37923 GN=AN277_0202340 PE=4 SV=1
MM1 pKa = 7.34SPRR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84RR8 pKa = 11.84SRR10 pKa = 11.84SRR12 pKa = 11.84PRR14 pKa = 11.84PRR16 pKa = 11.84VRR18 pKa = 11.84MSTRR22 pKa = 11.84TRR24 pKa = 11.84FIVTVVAVVVLVVVGLGTRR43 pKa = 11.84GLDD46 pKa = 3.37LFRR49 pKa = 11.84GEE51 pKa = 5.25DD52 pKa = 3.63GSADD56 pKa = 3.61RR57 pKa = 11.84SGATASRR64 pKa = 11.84AAGPLPAGLTAQQALDD80 pKa = 3.6SLEE83 pKa = 4.44TKK85 pKa = 10.52GKK87 pKa = 9.83AARR90 pKa = 11.84TGYY93 pKa = 10.64SRR95 pKa = 11.84DD96 pKa = 3.28AFGARR101 pKa = 11.84WADD104 pKa = 3.35VDD106 pKa = 5.44HH107 pKa = 6.85NGCDD111 pKa = 3.32TRR113 pKa = 11.84NDD115 pKa = 2.97ILARR119 pKa = 11.84DD120 pKa = 4.17LTQTTLRR127 pKa = 11.84PGTCKK132 pKa = 10.37VATGVLQDD140 pKa = 4.2PYY142 pKa = 9.64TGRR145 pKa = 11.84RR146 pKa = 11.84IDD148 pKa = 3.75FTAGAQTSAAVQIDD162 pKa = 4.12HH163 pKa = 6.65VVALSDD169 pKa = 3.15AWQTGAQQLSTADD182 pKa = 3.82RR183 pKa = 11.84MALANDD189 pKa = 4.26PLNLLAVDD197 pKa = 4.99GPANNGKK204 pKa = 9.89SDD206 pKa = 3.55GDD208 pKa = 3.62AATWLPEE215 pKa = 4.0QKK217 pKa = 10.16SFRR220 pKa = 11.84CEE222 pKa = 3.58YY223 pKa = 9.74VARR226 pKa = 11.84QAGVKK231 pKa = 9.57RR232 pKa = 11.84KK233 pKa = 9.41YY234 pKa = 10.38DD235 pKa = 3.33LWVTRR240 pKa = 11.84AEE242 pKa = 4.0RR243 pKa = 11.84AAMQRR248 pKa = 11.84VLDD251 pKa = 3.88TCPGQRR257 pKa = 11.84IPTRR261 pKa = 3.58

Molecular weight: 28.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1872

0

1872

632351

37

1820

337.8

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.238 ± 0.088

0.632 ± 0.014

5.7 ± 0.052

6.431 ± 0.064

2.843 ± 0.033

9.207 ± 0.057

2.151 ± 0.025

4.064 ± 0.044

2.071 ± 0.052

10.281 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.024

1.742 ± 0.03

5.663 ± 0.048

3.437 ± 0.033

7.969 ± 0.071

5.261 ± 0.04

5.672 ± 0.039

8.238 ± 0.047

1.456 ± 0.022

1.975 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski