Cicer arietinum (Chickpea) (Garbanzo)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q7Y697|A0A3Q7Y697_CICAR Isoform of A0A3Q7XQD1 B3 domain-containing protein LOC_Os12g40080-like isoform X2 OS=Cicer arietinum OX=3827 GN=LOC101504675 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.67LPEE5 pKa = 4.86PPTGFVLDD13 pKa = 4.41DD14 pKa = 4.11NGTVTLSTTNRR25 pKa = 11.84LATIVDD31 pKa = 5.11PISNLPLEE39 pKa = 4.39CVIRR43 pKa = 11.84RR44 pKa = 11.84VFKK47 pKa = 11.0SSDD50 pKa = 3.23RR51 pKa = 11.84DD52 pKa = 3.34EE53 pKa = 5.72CMLLCPVDD61 pKa = 3.83TPVQILKK68 pKa = 8.43STRR71 pKa = 11.84DD72 pKa = 3.13GWSAISDD79 pKa = 3.84EE80 pKa = 4.52EE81 pKa = 4.51DD82 pKa = 3.64EE83 pKa = 4.76SVLPAAAYY91 pKa = 10.03ALAKK95 pKa = 10.06IHH97 pKa = 5.5MHH99 pKa = 6.11LVYY102 pKa = 10.55SGYY105 pKa = 10.69CYY107 pKa = 8.81TARR110 pKa = 11.84GGFCYY115 pKa = 9.84TDD117 pKa = 3.76KK118 pKa = 11.16DD119 pKa = 3.41ILDD122 pKa = 3.74FHH124 pKa = 6.76TDD126 pKa = 3.2DD127 pKa = 4.01GKK129 pKa = 11.28EE130 pKa = 3.97VDD132 pKa = 4.09GLPSEE137 pKa = 4.5GVEE140 pKa = 3.41ITYY143 pKa = 9.61FYY145 pKa = 11.06LKK147 pKa = 8.62NTRR150 pKa = 11.84YY151 pKa = 10.13LIYY154 pKa = 10.01TPPEE158 pKa = 3.7PVQFVVVKK166 pKa = 10.46DD167 pKa = 3.9EE168 pKa = 4.29NGMFQMADD176 pKa = 3.92DD177 pKa = 5.39DD178 pKa = 5.33LLDD181 pKa = 4.61DD182 pKa = 4.68PAVIDD187 pKa = 4.91SIDD190 pKa = 3.43EE191 pKa = 4.07EE192 pKa = 5.09TEE194 pKa = 3.85FNALVEE200 pKa = 4.23EE201 pKa = 4.25EE202 pKa = 4.26AAVIEE207 pKa = 4.18ALMDD211 pKa = 4.08DD212 pKa = 4.76EE213 pKa = 5.02EE214 pKa = 6.48DD215 pKa = 3.71SDD217 pKa = 4.79EE218 pKa = 4.22EE219 pKa = 4.72DD220 pKa = 4.34YY221 pKa = 11.5YY222 pKa = 12.01

Molecular weight: 24.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2YD42|A0A1S2YD42_CICAR CASP-like protein OS=Cicer arietinum OX=3827 GN=LOC101515680 PE=3 SV=1
MM1 pKa = 7.18FGSVRR6 pKa = 11.84IVSNIAACIRR16 pKa = 11.84QNLSLHH22 pKa = 5.88GVQVRR27 pKa = 11.84NINIGGGLGGEE38 pKa = 4.27IPDD41 pKa = 4.25SKK43 pKa = 10.55RR44 pKa = 11.84LQYY47 pKa = 11.3ALQHH51 pKa = 5.12LHH53 pKa = 5.97GVGRR57 pKa = 11.84SKK59 pKa = 10.81AHH61 pKa = 6.88HH62 pKa = 6.25IVCEE66 pKa = 4.03LGVEE70 pKa = 4.11NKK72 pKa = 9.69YY73 pKa = 11.19VKK75 pKa = 10.43DD76 pKa = 3.54LSKK79 pKa = 11.16RR80 pKa = 11.84EE81 pKa = 4.04LYY83 pKa = 10.4SLRR86 pKa = 11.84EE87 pKa = 4.13LLSKK91 pKa = 10.61YY92 pKa = 10.85LIGNDD97 pKa = 3.18LKK99 pKa = 11.29KK100 pKa = 10.69LVEE103 pKa = 4.23RR104 pKa = 11.84DD105 pKa = 3.05VGRR108 pKa = 11.84LVGIQCYY115 pKa = 9.89RR116 pKa = 11.84GIRR119 pKa = 11.84HH120 pKa = 6.11VDD122 pKa = 3.25GLPCRR127 pKa = 11.84GQRR130 pKa = 11.84THH132 pKa = 6.03TNSRR136 pKa = 11.84TRR138 pKa = 11.84RR139 pKa = 11.84TMRR142 pKa = 11.84TFGGSRR148 pKa = 3.16

Molecular weight: 16.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24756

5866

30622

14024822

20

5450

458.0

51.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.099 ± 0.012

1.872 ± 0.007

5.361 ± 0.009

6.413 ± 0.017

4.311 ± 0.009

6.181 ± 0.017

2.522 ± 0.006

5.685 ± 0.01

6.403 ± 0.013

9.588 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.005

5.003 ± 0.01

4.726 ± 0.016

3.763 ± 0.01

4.942 ± 0.011

9.084 ± 0.017

5.029 ± 0.008

6.452 ± 0.009

1.244 ± 0.004

2.897 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski