Jatropha leaf curl Gujarat virus
Average proteome isoelectric point is 7.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U1ZZ75|A0A0U1ZZ75_9GEMI C4 protein OS=Jatropha leaf curl Gujarat virus OX=2169734 GN=C4 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.99 FPDD11 pKa = 3.86 SVHH14 pKa = 6.81 GFRR17 pKa = 11.84 CMLAVKK23 pKa = 9.82 YY24 pKa = 9.87 LQLVEE29 pKa = 4.41 GTYY32 pKa = 10.88 SPDD35 pKa = 3.47 TLGHH39 pKa = 5.47 EE40 pKa = 5.46 LIRR43 pKa = 11.84 DD44 pKa = 4.64 LISVIRR50 pKa = 11.84 AKK52 pKa = 10.73 NYY54 pKa = 10.36 AEE56 pKa = 3.87 ATSRR60 pKa = 11.84 YY61 pKa = 8.12 HH62 pKa = 6.83 HH63 pKa = 6.4 FHH65 pKa = 6.18 TRR67 pKa = 11.84 LEE69 pKa = 4.39 GSSPSEE75 pKa = 3.76 LRR77 pKa = 11.84 QPICQPCSCPYY88 pKa = 10.2 CPRR91 pKa = 11.84 HH92 pKa = 6.22 KK93 pKa = 10.6 KK94 pKa = 10.37 SGLDD98 pKa = 3.48 QQAHH102 pKa = 6.16 EE103 pKa = 4.51 SQAQVVQDD111 pKa = 3.67 LL112 pKa = 3.9
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.884
IPC2_protein 5.944
IPC_protein 6.046
Toseland 6.402
ProMoST 6.453
Dawson 6.402
Bjellqvist 6.351
Wikipedia 6.427
Rodwell 6.389
Grimsley 6.532
Solomon 6.402
Lehninger 6.402
Nozaki 6.664
DTASelect 6.854
Thurlkill 6.883
EMBOSS 6.868
Sillero 6.795
Patrickios 3.427
IPC_peptide 6.415
IPC2_peptide 6.781
IPC2.peptide.svr19 6.658
Protein with the highest isoelectric point:
>tr|A0A0U2A2S6|A0A0U2A2S6_9GEMI Capsid protein OS=Jatropha leaf curl Gujarat virus OX=2169734 GN=V1 PE=3 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.96 RR4 pKa = 11.84 PADD7 pKa = 3.82 IIISTPGSKK16 pKa = 9.66 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.99 SPYY27 pKa = 10.47 ASRR30 pKa = 11.84 AAAPTVRR37 pKa = 11.84 VTRR40 pKa = 11.84 SQAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 8.08 PKK54 pKa = 8.69 WYY56 pKa = 9.75 RR57 pKa = 11.84 IYY59 pKa = 10.66 RR60 pKa = 11.84 SPDD63 pKa = 3.09 VPKK66 pKa = 10.81 GCEE69 pKa = 4.39 GPCKK73 pKa = 10.05 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.68 VTRR97 pKa = 11.84 GTGFTHH103 pKa = 7.15 RR104 pKa = 11.84 VGKK107 pKa = 9.73 RR108 pKa = 11.84 FCVKK112 pKa = 9.92 SVYY115 pKa = 10.16 ILGKK119 pKa = 9.37 IWMDD123 pKa = 3.37 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.61 NHH131 pKa = 6.03 TNTVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.63 NMYY172 pKa = 9.53 RR173 pKa = 11.84 DD174 pKa = 3.71 RR175 pKa = 11.84 YY176 pKa = 8.85 QVLRR180 pKa = 11.84 KK181 pKa = 9.18 WSSTVTGGQYY191 pKa = 10.8 ASKK194 pKa = 9.43 EE195 pKa = 3.85 QAIVRR200 pKa = 11.84 RR201 pKa = 11.84 FFRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.531
IPC_protein 9.75
Toseland 10.072
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.628
Grimsley 10.379
Solomon 10.321
Lehninger 10.292
Nozaki 10.101
DTASelect 9.999
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 10.058
IPC_peptide 10.321
IPC2_peptide 8.887
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1089
100
352
181.5
20.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.418 ± 0.511
2.112 ± 0.459
5.234 ± 0.57
4.224 ± 0.379
4.04 ± 0.665
5.785 ± 0.552
3.765 ± 0.786
5.234 ± 0.755
4.683 ± 0.999
7.071 ± 1.418
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.488
5.234 ± 0.637
6.244 ± 0.578
4.591 ± 0.557
7.622 ± 0.863
8.632 ± 1.131
6.336 ± 0.959
6.061 ± 1.272
1.469 ± 0.209
4.04 ± 0.47
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here