Podoviridae sp. ctbh1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4GZ68|A0A2Z4GZ68_9CAUD Outer membrane beta-barrel domain protein OS=Podoviridae sp. ctbh1 OX=2675446 PE=4 SV=1
MM1 pKa = 7.37SVNPSLAAGLIALQAIANFLDD22 pKa = 3.66QGSGNATFIYY32 pKa = 10.5YY33 pKa = 10.7DD34 pKa = 3.27NTKK37 pKa = 9.05PANVGVAADD46 pKa = 3.66PTARR50 pKa = 11.84LVTLNLPEE58 pKa = 4.52PCFKK62 pKa = 10.93SLLSDD67 pKa = 4.69GIEE70 pKa = 4.93LYY72 pKa = 8.82PTDD75 pKa = 3.05IGMAIKK81 pKa = 10.59LGVVQWARR89 pKa = 11.84LYY91 pKa = 10.73NGAGVAVADD100 pKa = 4.34FAMGTDD106 pKa = 4.24IILNSYY112 pKa = 10.33DD113 pKa = 3.15IALGSSQKK121 pKa = 10.45LDD123 pKa = 3.84SIILKK128 pKa = 10.32LVVGG132 pKa = 4.47

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4GYX2|A0A2Z4GYX2_9CAUD Uncharacterized protein OS=Podoviridae sp. ctbh1 OX=2675446 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84IMVKK6 pKa = 10.22PLQLVNKK13 pKa = 8.59VQLRR17 pKa = 11.84QLVTTVQLRR26 pKa = 11.84QLVNKK31 pKa = 9.4VQLRR35 pKa = 11.84QQVTTVQLRR44 pKa = 11.84QLVNKK49 pKa = 9.28VQLRR53 pKa = 11.84QLVNKK58 pKa = 9.4VQLRR62 pKa = 11.84QQVTTVQLRR71 pKa = 11.84QLVNKK76 pKa = 9.4VQLRR80 pKa = 11.84QQVTTVQLRR89 pKa = 11.84QQVTTVQLRR98 pKa = 11.84QLVTEE103 pKa = 4.15VQLRR107 pKa = 11.84QQVKK111 pKa = 9.79KK112 pKa = 9.91VQLRR116 pKa = 11.84QLVNKK121 pKa = 9.4VQLRR125 pKa = 11.84QQVTTVQLRR134 pKa = 11.84QLVTTVQLRR143 pKa = 11.84QLVTEE148 pKa = 4.15VQLRR152 pKa = 11.84QQVTTVQLRR161 pKa = 11.84QQVTTVQLRR170 pKa = 11.84QQANIQSHH178 pKa = 4.8VVV180 pKa = 3.01

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13224

81

1073

244.9

27.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.212 ± 0.686

0.892 ± 0.126

5.777 ± 0.224

5.808 ± 0.356

4.015 ± 0.231

6.692 ± 0.459

1.724 ± 0.175

6.715 ± 0.266

6.828 ± 0.396

8.878 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.173

5.535 ± 0.246

3.433 ± 0.258

4.938 ± 0.39

4.167 ± 0.279

6.965 ± 0.322

6.307 ± 0.401

6.367 ± 0.331

1.255 ± 0.131

3.168 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski