Streptococcus phage 33888

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F7D0|A0A3G8F7D0_9CAUD Uncharacterized protein OS=Streptococcus phage 33888 OX=2484226 GN=33888_00053 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 5.52RR3 pKa = 11.84GLFGTFDD10 pKa = 3.62YY11 pKa = 11.26DD12 pKa = 3.99RR13 pKa = 11.84DD14 pKa = 3.88YY15 pKa = 11.38LQPPEE20 pKa = 4.2PRR22 pKa = 11.84EE23 pKa = 3.96EE24 pKa = 4.13RR25 pKa = 11.84DD26 pKa = 3.18PADD29 pKa = 3.38WLFSAGQWIYY39 pKa = 10.84IGDD42 pKa = 3.93CC43 pKa = 3.48

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F795|A0A3G8F795_9CAUD Minor capsid protein OS=Streptococcus phage 33888 OX=2484226 GN=33888_00023 PE=4 SV=1
MM1 pKa = 7.42GGVNVKK7 pKa = 9.77IDD9 pKa = 3.46LSGVEE14 pKa = 4.47KK15 pKa = 10.58KK16 pKa = 10.78VSPEE20 pKa = 3.45NFAKK24 pKa = 10.68GKK26 pKa = 9.91LAIANQMLMDD36 pKa = 4.12MEE38 pKa = 4.51RR39 pKa = 11.84FVPKK43 pKa = 10.25RR44 pKa = 11.84RR45 pKa = 11.84GDD47 pKa = 3.58LRR49 pKa = 11.84SSGHH53 pKa = 5.14VRR55 pKa = 11.84QDD57 pKa = 3.5SIVYY61 pKa = 6.47ATPYY65 pKa = 10.94ARR67 pKa = 11.84LLYY70 pKa = 10.06YY71 pKa = 10.27GKK73 pKa = 9.95KK74 pKa = 9.48RR75 pKa = 11.84KK76 pKa = 9.93GFFSEE81 pKa = 3.79KK82 pKa = 8.59QRR84 pKa = 11.84RR85 pKa = 11.84FFFANKK91 pKa = 9.99DD92 pKa = 3.29KK93 pKa = 11.12LLSQKK98 pKa = 7.5PTPGTGPRR106 pKa = 11.84WDD108 pKa = 3.86KK109 pKa = 10.91KK110 pKa = 11.03ASALYY115 pKa = 9.24AKK117 pKa = 9.95KK118 pKa = 9.55WADD121 pKa = 3.39VGAKK125 pKa = 9.38TMGVKK130 pKa = 10.07

Molecular weight: 14.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

10750

33

1071

188.6

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.921 ± 0.681

0.493 ± 0.095

6.605 ± 0.415

7.526 ± 0.521

4.149 ± 0.225

6.335 ± 0.457

1.367 ± 0.145

7.498 ± 0.405

7.991 ± 0.548

8.298 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.195

5.367 ± 0.243

2.744 ± 0.195

4.428 ± 0.292

4.14 ± 0.309

6.567 ± 0.505

5.553 ± 0.273

6.242 ± 0.277

1.181 ± 0.153

3.833 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski